Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination (Q92292201)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:31128791 AND SRC:MED&resulttype=core&format=json

28 April 2020

title

Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination (English)

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28 April 2020

1 reference

1

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28 April 2020

Kazuya Yamanaka

series ordinal

2

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28 April 2020

Xiaoyu Tang

series ordinal

3

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28 April 2020

4

Bradley S Moore

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28 April 2020

publication date

21 March 2019

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28 April 2020

published in

Methods in Enzymology

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28 April 2020

volume

621

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28 April 2020

page(s)

87-110

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Triggering the expression of a silent gene cluster from genetically intractable bacteria results in scleric acid discovery

28 April 2020

cites work

1 reference

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Clone of Plipastatin Biosynthesis Gene Cluster by Transformation-associated Recombination Technique and High Efficient Expression in Model Organism Bacillus subtilis

23 February 2021

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Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters

23 February 2021

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Characterization of the Jomthonic Acids Biosynthesis Pathway and Isolation of Novel Analogues in GUA-06-05-006A

23 February 2021

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Biosynthesis of antibiotic chuangxinmycin from Actinoplanes tsinanensis.

23 February 2021

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Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster

23 February 2021

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TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in Streptomyces aureofaciens

23 February 2021

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Broad-Host-Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram-Negative Bacteria

23 February 2021

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Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method.

23 February 2021

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PCR-Independent Method of Transformation-Associated Recombination Reveals the Cosmomycin Biosynthetic Gene Cluster in an Ocean Streptomycete.

23 February 2021

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Biosynthetic Pathway Connects Cryptic Ribosomally Synthesized Posttranslationally Modified Peptide Genes with Pyrroloquinoline Alkaloids.

23 February 2021

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Divergent biosynthesis yields a cytotoxic aminomalonate-containing precolibactin

23 February 2021

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Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology

23 February 2021

1 reference

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New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

23 February 2021

1 reference

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A Peptidyl-Transesterifying Type I Thioesterase in Salinamide Biosynthesis

23 February 2021

1 reference

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Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining

23 February 2021

1 reference

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Reinvigorating natural product combinatorial biosynthesis with synthetic biology

23 February 2021

1 reference

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Computational approaches to natural product discovery

23 February 2021

1 reference

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Minimum Information about a Biosynthetic Gene cluster.

23 February 2021

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6555405

Genome Mining of Streptomyces sp. Tü 6176: Characterization of the Nataxazole Biosynthesis Pathway

23 February 2021

1 reference

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Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis

23 February 2021

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6555405

Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast.

23 February 2021

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6555405

Direct capture and heterologous expression of Salinispora natural product genes for the biosynthesis of enterocin.

23 February 2021

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6555405

Targeted capture and heterologous expression of the Pseudoalteromonas alterochromide gene cluster in Escherichia coli represents a promising natural product exploratory platform

23 February 2021

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6555405

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A.

23 February 2021

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6555405

An improved method for oriT-directed cloning and functionalization of large bacterial genomic regions

23 February 2021

1 reference

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Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.

23 February 2021

1 reference

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Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies

23 February 2021

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Cloning large natural product gene clusters from the environment: piecing environmental DNA gene clusters back together with TAR.

23 February 2021

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TAR cloning: insights into gene function, long-range haplotypes and genome structure and evolution

23 February 2021

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Exploiting the yeast Saccharomyces cerevisiae for the study of the organization and evolution of complex genomes

23 February 2021

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A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast

23 February 2021

1 reference

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Functional copies of a human gene can be directly isolated by transformation-associated recombination cloning with a small 3' end target sequence.

23 February 2021

1 reference

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Direct isolation of human BRCA2 gene by transformation-associated recombination in yeast

23 February 2021

1 reference

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Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination

23 February 2021

1 reference

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Yeast transformation: a model system for the study of recombination

23 February 2021

1 reference

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Expression of competence genes in Bacillus subtilis

23 February 2021

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6555405

10.1016/BS.MIE.2019.02.026

23 February 2021

Identifiers

DOI

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:31128791 AND SRC:MED&resulttype=core&format=json

28 April 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:31128791 AND SRC:MED&resulttype=core&format=json

28 April 2020

PubMed publication ID

31128791

1 reference