Polyketide synthase acyl carrier proteins from Streptomyces: expression in Escherichia coli, purification and partial characterisation (Q71988953)

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22 October 2019

title

Polyketide synthase acyl carrier proteins from Streptomyces: expression in Escherichia coli, purification and partial characterisation (English)

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22 October 2019

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J. Crosby

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13 July 2024

D. H. Sherman

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13 July 2024

M. J. Bibb

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W. P. Revill

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D. A. Hopwood

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T. J. Simpson

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language of work or name

English

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publication date

1 August 1995

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22 October 2019

16 August 1995

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National Center for Biotechnology Information

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=7647090

full work available at URL

13 July 2024

https://api.elsevier.com/content/article/PII:016748389500053W?httpAccept=text/xml

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https://api.elsevier.com/content/article/PII:016748389500053W?httpAccept=text/plain

13 July 2024

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Biochimica et Biophysica Acta

13 July 2024

published in

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22 October 2019

volume

1251

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22 October 2019

issue

1

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22 October 2019

page(s)

32-42

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Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tü22.

22 October 2019

cites work

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Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis.

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Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus

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Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by heterologous genes from a different polyketide pathway

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Overproduction and localization of components of the polyketide synthase of Streptomyces glaucescens involved in the production of the antibiotic tetracenomycin C

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The biosynthesis of polyketides

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Molecular genetics of polyketides and its comparison to fatty acid biosynthesis

13 July 2024

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Polyketide synthase com plexes: their structure and function in antibiotic biosynthesis

13 July 2024

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The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum. Its gene structure relative to that of other polyketide synthases

13 July 2024

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Structural organization of the multifunctional animal fatty-acid synthase

13 July 2024

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Sequences of the oxytetracycline polyketide synthase-encoding otc genes from Streptomyces rimosus

13 July 2024

13

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Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicin

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14

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Fatty acid synthase, a proficient multifunctional enzyme

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Fatty acid synthesis and its regulation

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Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein

13 July 2024

18

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Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins

13 July 2024

19

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Engineered biosynthesis of novel polyketides

13 July 2024

20

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Engineered biosynthesis of novel polyketides: stereochemical course of two reactions catalyzed by a polyketide synthase

13 July 2024

23

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Nucleotide sequences and heterologous expression of tcmG and tcmP, biosynthetic genes for tetracenomycin C in Streptomyces glaucescens

13 July 2024

25

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Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2)

13 July 2024

29

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Production of single-stranded plasmid DNA

13 July 2024

33

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Use of electrospray mass spectrometry to assay chemical reactivity of an acyl carrier protein involved in fatty acid biosynthesis in Saccharopolyspora erythraea

13 July 2024

34

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Use of electrospray mass spectrometry in enzymic studies on acyl carrier protein implicated in fatty acid biosynthesis in Saccharopolyspora erythraea

13 July 2024

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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Transcriptional regulation of the redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)

13 July 2024

37

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Cloning, characterization, and high-level expression in Escherichia coli of the Saccharopolyspora erythraea gene encoding an acyl carrier protein potentially involved in fatty acid biosynthesis

13 July 2024

38

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Purification and characterization of the acyl carrier protein of the Streptomyces glaucescens tetracenomycin C polyketide synthase

13 July 2024

39

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Codon usage in the G C-rich Streptomyces genome

13 July 2024

40

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Synthesis, cloning, and expression in Escherichia coli of a spinach acyl carrier protein-I gene

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Expression of an active spinach acyl carrier protein-I/protein-A gene fusion

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Characterization of recombinant thioesterase and acyl carrier protein domains of chicken fatty acid synthase expressed in Escherichia coli.

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An acyl-carrier-protein-thioesterase domain from the 6-deoxyerythronolide B synthase of Saccharopolyspora erythraea. High-level production, purification and characterisation in Escherichia coli

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Purification and separation of holo- and apo-forms of Saccharopolyspora erythraea acyl-carrier protein released from recombinant Escherichia coli by freezing and thawing

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A small, discrete acyl carrier protein is involved in de novo fatty acid biosynthesis in Streptomyces erythraeus.

13 July 2024

47

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Three-dimensional structure of acyl carrier protein in solution determined by nuclear magnetic resonance and the combined use of dynamical simulated annealing and distance geometry.

13 July 2024

49

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Refinement of the NMR structures for acyl carrier protein with scalar coupling data

13 July 2024

50

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13 July 2024

Identifiers

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22 October 2019

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