A universal expression-purification system based on the coiled-coil interaction of myosin heavy chain (Q54675559)

2 June 2018

title

A universal expression-purification system based on the coiled-coil interaction of myosin heavy chain (English)

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19 September 2022

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19 September 2022

author name string

V. Wolber

1

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19 September 2022

K. Maeda

2

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19 September 2022

R. Schumann

3

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19 September 2022

B. Brandmeier

4

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19 September 2022

L. Wiesmüller

5

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19 September 2022

A. Wittinghofer

6

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19 September 2022

language of work or name

English

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National Center for Biotechnology Information

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=1368985

19 September 2022

publication date

1 August 1992

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2 June 2018

published in

Bio/technology

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2 June 2018

volume

10

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2 June 2018

issue

8

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2 June 2018

page(s)

900-904

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2 June 2018

Plasmid vectors for high-efficiency expression controlled by the promoter of coliphage lambda

cites work

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The purification of eukaryotic polypeptides synthesized in Escherichia coli

21 January 2018

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New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli

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Gene fusion vectors based on the gene for staphylococcal protein A.

21 January 2018

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Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

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Cloning of bovine GAP and its interaction with oncogenic ras p21.

21 January 2018

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Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: the C-terminal domain of GAP is not sufficient for full activity

21 January 2018

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The catalytic domain of the neurofibromatosis type 1 gene product stimulates ras GTPase and complements ira mutants of S. cerevisiae

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The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins

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Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1

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Complete mutagenesis of the HIV-1 protease

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Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli

21 January 2018

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7 January 2021

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes

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Open reading frame cloning: identification, cloning, and expression of open reading frame DNA.

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Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase

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Production in Escherichia coli and one-step purification of bifunctional hybrid proteins which bind maltose. Export of the Klenow polymerase into the periplasmic space

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7 January 2021

An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

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7 January 2021

Rabbit skeletal muscle myosin. Unfolded carboxyl-terminus and its role in molecular assembly

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7 January 2021

C-terminal structure and mobility of rabbit skeletal muscle light meromyosin as studied by one- and two-dimensional 1H NMR spectroscopy and X-ray small-angle scattering

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7 January 2021

Characterization of cDNA coding for the complete light meromyosin portion of a rabbit fast skeletal muscle myosin heavy chain

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7 January 2021

"ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli

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A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants

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Effect of adenosine di- and triphosphates on the stability of synthetic myosin filaments.

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7 January 2021

Structural changes in synthetic myosin minifilaments and their dissociation by adenosine triphosphate and pyrophosphate

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7 January 2021

Monomers, dimers, and minifilaments of vertebrate skeletal myosin in the presence of sodium pyrophosphate

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Trans-activator gene of human T-lymphotropic virus type III (HTLV-III).

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Location of the trans-activating region on the genome of human T-cell lymphotropic virus type III.

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7 January 2021

Tat protein from human immunodeficiency virus forms a metal-linked dimer

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7 January 2021

Fragments of the HIV-1 Tat protein specifically bind TAR RNA.

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Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli

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Isolation of mutants of human immunodeficiency virus protease based on the toxicity of the enzyme in Escherichia coli

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Antibodies to recombinant HIV-1 vif, tat, and nef proteins in human sera

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Inhibition of GTPase activating protein stimulation of Ras-p21 GTPase by the Krev-1 gene product

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Identifiers

DOI

10.1038/NBT0892-900

1 reference

2 June 2018

1 reference

2 June 2018