Quantifying cellular capacity identifies gene expression designs with reduced burden (Q48205353)

9 February 2018

title

Quantifying cellular capacity identifies gene expression designs with reduced burden (English)

1 reference

9 February 2018

author

Thomas Ellis

4

1 reference

9 February 2018

Guy-Bart Stan

object named as

Guy-Bart Stan

3

0 references

Francesca Ceroni

object named as

Francesca Ceroni

1

0 references

author name string

Rhys Algar

2

1 reference

9 February 2018

publication date

6 April 2015

1 reference

9 February 2018

published in

Nature Methods

1 reference

9 February 2018

volume

12

1 reference

9 February 2018

issue

5

1 reference

9 February 2018

page(s)

415-418

1 reference

9 February 2018

Synthetic biology--putting engineering into biology

cites work

1 reference

Tuning the dials of Synthetic Biology

21 January 2018

1 reference

Principles of genetic circuit design

21 January 2018

1 reference

Cost of unneeded proteins in E. coli is reduced after several generations in exponential growth

21 January 2018

1 reference

Using synthetic biological parts and microbioreactors to explore the protein expression characteristics of Escherichia coli.

21 January 2018

1 reference

Contextualizing context for synthetic biology--identifying causes of failure of synthetic biological systems

21 January 2018

1 reference

Visualization of evolutionary stability dynamics and competitive fitness of Escherichia coli engineered with randomized multigene circuits

21 January 2018

1 reference

Genetic circuit performance under conditions relevant for industrial bioreactors

21 January 2018

1 reference

Emergent bistability by a growth-modulating positive feedback circuit

21 January 2018

1 reference

Effects of genetic variation on the E. coli host-circuit interface

21 January 2018

1 reference

Gene circuit performance characterization and resource usage in a cell-free "breadboard".

21 January 2018

1 reference

Growth rate-dependent global effects on gene expression in bacteria

21 January 2018

1 reference

Interdependence of cell growth and gene expression: origins and consequences.

21 January 2018

1 reference

Empirical model and in vivo characterization of the bacterial response to synthetic gene expression show that ribosome allocation limits growth rate.

21 January 2018

1 reference

Dissecting specific and global transcriptional regulation of bacterial gene expression

21 January 2018

1 reference

Synthesis of proteins in Escherichia coli is limited by the concentration of free ribosomes. Expression from reporter genes does not always reflect functional mRNA levels.

21 January 2018

1 reference

Translation initiation rate determines the impact of ribosome stalling on bacterial protein synthesis.

21 January 2018

1 reference

The centrality of RNA for engineering gene expression

21 January 2018

1 reference

Synthetic analog computation in living cells.

21 January 2018

1 reference

Genetic programs constructed from layered logic gates in single cells

21 January 2018

1 reference

Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations

21 January 2018

1 reference

Construction of a genetic toggle switch in Escherichia coli

21 January 2018

1 reference

Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria

21 January 2018

1 reference

Engineering and characterization of a superfolder green fluorescent protein

21 January 2018

1 reference

Engineering BioBrick vectors from BioBrick parts

21 January 2018

1 reference

Refinement and standardization of synthetic biological parts and devices

21 January 2018

1 reference

Enzymatic assembly of DNA molecules up to several hundred kilobases

21 January 2018

1 reference

A flow cytometry-based screen for synthetic riboswitches

21 January 2018

1 reference

The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases

21 January 2018

1 reference

Design of the linkers which effectively separate domains of a bifunctional fusion protein.

21 January 2018

1 reference

The Ribosome Binding Site Calculator

21 January 2018

1 reference

Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries

21 January 2018

1 reference

Design parameters to control synthetic gene expression in Escherichia coli

21 January 2018

1 reference

Synonymous but not the same: the causes and consequences of codon bias

21 January 2018

1 reference

Ribosome collisions and translation efficiency: optimization by codon usage and mRNA destabilization

21 January 2018

1 reference

Amino acid content of recombinant proteins influences the metabolic burden response

21 January 2018

1 reference

The anti-Shine-Dalgarno sequence drives translational pausing and codon choice in bacteria

21 January 2018

1 reference

Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources

21 January 2018

1 reference

The role of codon selection in regulation of translation efficiency deduced from synthetic libraries

21 January 2018

1 reference

Causes and effects of N-terminal codon bias in bacterial genes

21 January 2018

1 reference

JCat: a novel tool to adapt codon usage of a target gene to its potential expression host

21 January 2018

1 reference

OPTIMIZER: a web server for optimizing the codon usage of DNA sequences

21 January 2018

1 reference

Stress induced by recombinant protein production in Escherichia coli

21 January 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/25849635

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1038/NMETH.3339

1 reference

9 February 2018

1 reference

9 February 2018