Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA. (Q47854748)

6 February 2018

title

Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA. (English)

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6 February 2018

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Collins-Racie LA

1

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6 February 2018

McColgan JM

2

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6 February 2018

Grant KL

3

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6 February 2018

DiBlasio-Smith EA

4

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6 February 2018

McCoy JM

5

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6 February 2018

LaVallie ER

6

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6 February 2018

publication date

1 September 1995

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6 February 2018

published in

Bio/technology

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6 February 2018

volume

13

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issue

9

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6 February 2018

page(s)

982-987

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6 February 2018

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

cites work

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FORMATION OF TRYPSIN FROM CRYSTALLINE TRYPSINOGEN BY MEANS OF ENTEROKINASE

21 January 2018

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Crystal structure of the DsbA protein required for disulphide bond formation in vivo

21 January 2018

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Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

21 January 2018

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Hydrolysis of artificial substrates by enterokinase and trypsin and the development of a sensitive specific assay for enterokinase in serum

21 January 2018

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Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction

21 January 2018

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An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein

21 January 2018

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7 January 2021

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

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7 January 2021

Thrombin specificity. Requirement for apolar amino acids adjacent to the thrombin cleavage site of polypeptide substrate

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7 January 2021

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

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7 January 2021

Identification of a protein required for disulfide bond formation in vivo

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7 January 2021

Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus

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7 January 2021

Expression of atrial natriuretic factor as a cleavable fusion protein with chloramphenicol acetyltransferase in Escherichia coli

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7 January 2021

An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I

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7 January 2021

Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid

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7 January 2021

Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli.

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7 January 2021

An expression system for trypsin.

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7 January 2021

Regulation of PACE propeptide-processing activity: requirement for a post-endoplasmic reticulum compartment and autoproteolytic activation

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7 January 2021

Specificity of bovine enterokinase toward protein substrates

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7 January 2021

High efficiency transformation of E. coli by high voltage electroporation

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7 January 2021

Preparation of genomic DNA from bacteria

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7 January 2021

Calculation of protein extinction coefficients from amino acid sequence data

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7 January 2021

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

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7 January 2021

Identifiers

DOI

10.1038/NBT0995-982

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6 February 2018

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6 February 2018