Split-inteins and their bioapplications (Q38543782)

4 September 2017

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title

Split-inteins and their bioapplications (English)

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4 September 2017

Yifeng Li

1

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4 September 2017

publication date

8 July 2015

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4 September 2017

published in

Biotechnology Letters

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4 September 2017

volume

37

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4 September 2017

issue

11

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4 September 2017

page(s)

2121-2137

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Novel split intein for trans-splicing synthetic peptide onto C terminus of protein

4 September 2017

cites work

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Highly Efficient and More General cis- and trans-Splicing Inteins through Sequential Directed Evolution

21 January 2018

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In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins

21 January 2018

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Structure-based engineering and comparison of novel split inteins for protein ligation

21 January 2018

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Chemical-tag labeling of proteins using fully recombinant split inteins

21 January 2018

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Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein

21 January 2018

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In-Cell Fluorescence Activation and Labeling of Proteins Mediated by FRET-Quenched Split Inteins

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources

21 January 2018

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Distribution of split DnaE inteins in cyanobacteria

21 January 2018

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Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Split-inteins for simultaneous, site-specific conjugation of quantum dots to multiple protein targets in vivo.

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Trans protein splicing of cyanobacterial split inteins in endogenous and exogenous combinations.

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Synthesis of proteins by native chemical ligation

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution

21 January 2018

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Modification of transmembrane and GPI-anchored proteins on living cells by efficient protein trans-splicing using the Npu DnaE intein

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag to Proteins Using Protein Trans-Splicing

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Self-cleavable stimulus responsive tags for protein purification without chromatography

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Inteins: structure, function, and evolution

21 January 2018

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https://api.crossref.org/works/10.1007/S10529-015-1905-2

Activation of specific apoptotic caspases with an engineered small-molecule-activated protease

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Split intein mediated ultra-rapid purification of tagless protein (SIRP).

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Intein-mediated protein trans-splicing and transgene containment in plastids

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Site-specific chemical modification of proteins with a prelabelled cysteine tag using the artificially split Mxe GyrA intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Circular permutation prediction reveals a viable backbone disconnection for split proteins: an approach in identifying a new functional split intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Self-cleaving fusion tags for recombinant protein production

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein trans-splicing as a means for viral vector-mediated in vivo gene therapy

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Two- and three-input TALE-based AND logic computation in embryonic stem cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein trans-splicing of multiple atypical split inteins engineered from natural inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Traceless protein splicing utilizing evolved split inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

A tunable zinc finger-based framework for Boolean logic computation in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Ligation of synthetic peptides to proteins using semisynthetic protein trans-splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein splicing triggered by a small molecule

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Expressed protein ligation: A general method for protein engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Semisynthetic murine prion protein equipped with a GPI anchor mimic incorporates into cellular membranes

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

A genetic method to identify mitochondrial proteins in living mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

A genetic approach to identifying mitochondrial proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein splicing and related forms of protein autoprocessing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

InBase, the Intein Database

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

A split intein T7 RNA polymerase for transcriptional AND-logic.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Probing intein-catalyzed thioester formation by unnatural amino acid substitutions in the active site

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Production of cyclic peptides and proteins in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein scaffold-activated protein trans-splicing in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Inteins: Nature's Gift to Protein Chemists

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Development of a tandem protein trans-splicing system based on native and engineered split inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein trans-splicing of an atypical split intein showing structural flexibility and cross-reactivity

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Control of protein splicing by intein fragment reassembly.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Manipulating L-type calcium channels in cardiomyocytes using split-intein protein transsplicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein trans-splicing to produce herbicide-resistant acetolactate synthase

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Synthetic two-piece and three-piece split inteins for protein trans-splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Establishment of intein-mediated protein ligation under denaturing conditions: C-terminal labeling of a single-chain antibody for biochip screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

An atypical naturally split intein engineered for highly efficient protein labeling.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Biological Applications of Protein Splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Activation of protein splicing by protease- or light-triggered O to N acyl migration

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Recent progress in intein research: from mechanism to directed evolution and applications

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Controllable protein cleavages through intein fragment complementation

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Site‐specific protein cleavage in vivo by an intein‐derived protease

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Gene-splitting technology: a novel approach for the containment of transgene flow in Nicotiana tabacum

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Structure-activity studies on the upstream splice junction of a semisynthetic intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Expression of split dnaE genes and trans-splicing of DnaE intein in the developmental cyanobacterium Anabaena sp. PCC 7120.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Split-intein mediated re-assembly of genetically encoded Ca2 indicators

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Simultaneous assembly of two target proteins using split inteins for live cell imaging

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Protein trans -splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Segmental labeling to study multidomain proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Site-specific two-color protein labeling for FRET studies using split inteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

SPLICEFINDER - a fast and easy screening method for active protein trans-splicing positions

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Circular β-lactamase: stability enhancement by cyclizing the backbone

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Highly efficient proteintrans-splicing by a naturally split DnaE intein fromNostoc punctiforme

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans-Splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007/S10529-015-1905-2

10.1007/S10529-015-1905-2

21 January 2018

Identifiers

DOI

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4 September 2017

1 reference