Electrophoretic assay for DNA-binding proteins (Q38349910)

2 September 2017

title

Electrophoretic assay for DNA-binding proteins (English)

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2 September 2017

Varshavsky A

1

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2 September 2017

language of work or name

English

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publication date

1 January 1987

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2 September 2017

published in

Methods in Enzymology

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2 September 2017

volume

151

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2 September 2017

page(s)

551-565

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Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis

2 September 2017

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Nucleosomes and subnucleosomes: heterogeneity and composition

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A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.

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A boundary sedimentation velocity method for determining nonspecific nucleic acid-protein interaction binding parameters

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7 January 2021

Direct measurement of association constants for the binding of Escherichia coli lac repressor to non-operator DNA

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7 January 2021

Measurement of macromolecular equilibrium binding constants by a sucrose gradient band sedimentation method. Application to protein-nucleic acid interactions

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Use of difference boundary sedimentation velocity to investigate nonspecific protein-nucleic acid interactions

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7 January 2021

Specific Binding of the λ Phage Repressor to λ DNA

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Measurement of DNA-protein equilibria using gel chromatography: application to the HinfI restriction endonuclease.

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7 January 2021

The detection of DNA-binding proteins by protein blotting

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7 January 2021

The DNA-protein cross: a method for detecting specific DNA-protein complexes in crude mixtures.

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Binding of a simian virus 40 T antigen-related protein to DNA

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A repressor (MAT alpha 2 Product) and its operator control expression of a set of cell type specific genes in yeast

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The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity

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Nonspecific interaction of lac repressor with DNA: an association reaction driven by counterion release.

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The high affinity binding site on polyoma virus DNA for the viral large-T protein

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7 January 2021

Lac repressor-operator interaction. I. Equilibrium studies

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7 January 2021

Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection.

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7 January 2021

Specificity of the weak binding between the phage SPO1 transcription-inhibitory protein, TF1, and SPO1 DNA

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Binding of Escherichia coli ribonucleic acid polymerase holoenzyme to a bacteriophage T7 promoter-containing fragment: selectivity exists over a wide range of solution conditions

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7 January 2021

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Purification of a cellular, double-stranded DNA-binding protein required for initiation of adenovirus DNA replication by using a rapid filter-binding assay

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7 January 2021

DNAase footprinting a simple method for the detection of protein-DNA binding specificity

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7 January 2021

The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter

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7 January 2021

Transcription factor Sp1 recognizes a DNA sequence in the mouse dihydrofolate reductase promoter

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7 January 2021

E. coli RNA polymerase interacts homologously with two different promoters

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7 January 2021

Mapping of SV40 DNA replication origin region binding sites for the SV40 T antigen by protection against exonuclease III digestion

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7 January 2021

The coupled use of ‘footprinting’ and exonuclease III methodology for RNA polymerase binding and initiation. Application for the analysis of three tandem promoters at the control region of colicin EI

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7 January 2021

Eight different highly specific nucleosome phases on α-satellite DNA in the African green monkey

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7 January 2021

An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts.

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7 January 2021

Multiple specific contacts between a mammalian transcription factor and its cognate promoters

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7 January 2021

B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo

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7 January 2021

Use of light for footprinting DNA in vivo

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7 January 2021

A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts

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7 January 2021

Equilibrium studies of the cyclic AMP receptor protein-DNA interaction

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7 January 2021

Kinetics and mechanism in the reaction of gene regulatory proteins with DNA

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https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Comparison of nucleic acid-protein interactions in solution and in polyacrylamide gels

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https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Identification of proteins involved in the regulation of yeast iso- 1-cytochrome C expression by oxygen.

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7 January 2021

A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes

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7 January 2021

An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.

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7 January 2021

Sequences required for in vitro transcriptional activation of a Drosophila hsp 70 gene

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7 January 2021

Selective proteolysis defines two DNA binding domains in yeast transcription factor tau

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7 January 2021

Distinct factors bind to apparently homolgous sequences in the immunoglobulin heavy-chain enhancer

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https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors

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https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Multiple nuclear factors interact with the immunoglobulin enhancer sequences

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https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Identification of a cellular transcription factor involved in E1A trans-activation

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https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Domains of the positive transcription factor specific for the Xenopus 5S RNA gene

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7 January 2021

Transcription of class III genes: formation of preinitiation complexes

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https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Contact points between a positive transcription factor and the Xenopus 5S RNA gene

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https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Kinetics of RNA polymerase-promoter complex formation: effects of nonspecific DNA-protein interactions

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Intermediates in transcription initiation from the E. coli lac UV5 promoter

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

The DNA binding domain and bending angle of E. coli CAP protein.

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

CAP and RNA polymerase interactions with thelacpromoter: binding stoichiometry and long range effects

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

H1a, an E. coli DNA-binding protein which accumulates in stationary phase, strongly compacts DNA in vitro

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Nucleosome cores have two specific binding sites for nonhistone chromosomal proteins HMG 14 and HMG 17

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

The interaction of high mobility proteins HMG14 and 17 with nucleosomes

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Affinity of HMG17 for a mononucleosome is not influenced by the presence of ubiquitin-H2A semihistone but strongly depends on DNA fragment size

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

1 reference

https://api.crossref.org/works/10.1016/S0076-6879(87)51044-8

7 January 2021

10.1016/S0076-6879(87)51044-8

Identifiers

DOI

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2 September 2017

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