Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy. (Q37751194)

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2 February 2020

review article

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title

Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy (English)

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1

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Stefan Diez

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Claire T. Friel

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Erik Schäffer

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Anastasiya Trushko

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Vladimir Varga

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Per O Widlund

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Marija Zanic

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Jonathon Howard

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Jonne Helenius

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author name string

Volker Bormuth

2

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2 February 2020

Gary J Brouhard

3

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Daniel N Cohen

4

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Bert Nitzsche

8

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Heike Petzold

9

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Jan Ribbe

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Jeffrey H Stear

12

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publication date

1 January 2010

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2 February 2020

published in

Methods in Cell Biology

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volume

95

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page(s)

221-245

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An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments

2 February 2020

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Total internal reflection fluorescence

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Reconstitution of a microtubule plus-end tracking system in vitro

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The free energy for hydrolysis of a microtubule-bound nucleotide triphosphate is near zero: all of the free energy for hydrolysis is stored in the microtubule lattice

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Evidence that a single monolayer tubulin-GTP cap is both necessary and sufficient to stabilize microtubules

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A comparison of optical geometries for combined flash photolysis and total internal reflection fluorescence microscopy

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Motility of dimeric ncd on a metal-chelating surfactant: evidence that ncd is not processive

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On/off blinking and switching behaviour of single molecules of green fluorescent protein

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Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution

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Phase diagram of microtubules

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An engineered protein tag for multiprotein labeling in living cells

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TIRF microscopy evanescent field calibration using tilted fluorescent microtubules

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Mechanism and dynamics of breakage of fluorescent microtubules

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The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends

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Growth, fluctuation and switching at microtubule plus ends

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Preparation of modified tubulins

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Role of GTP hydrolysis in microtubule dynamics: information from a slowly hydrolyzable analogue, GMPCPP

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Detection of fractional steps in cargo movement by the collective operation of kinesin-1 motors

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Rigidity of microtubules is increased by stabilizing agents

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Dynamic instability of microtubule growth

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How kinesin waits between steps

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Ionic and nucleotide requirements for microtubule polymerization in vitro

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Nonblinking and long-lasting single-molecule fluorescence imaging

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Biotemplated Nanopatterning of Planar Surfaces with Molecular Motors

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7 January 2021

Total internal reflection with fluorescence correlation spectroscopy

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7 January 2021

Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner

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Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations

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Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization

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Identifiers

DOI

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2 February 2020

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