Microinjection of ubiquitin: intracellular distribution and metabolism in HeLa cells maintained under normal physiological conditions (Q36216320)

12 August 2017

Microinjection of ubiquitin: intracellular distribution and metabolism in HeLa cells maintained under normal physiological conditions (English)

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12 August 2017

N Carlson

1

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12 August 2017

M Rechsteiner

series ordinal

2

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12 August 2017

language of work or name

English

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publication date

1 March 1987

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12 August 2017

Journal of Cell Biology

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12 August 2017

104

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12 August 2017

3

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12 August 2017

537-546

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CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM

12 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Direct visualization of fluorescein-labeled microtubules in vitro and in microinjected fibroblasts

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Degradation of proteins microinjected into IMR-90 human diploid fibroblasts

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Pattern and time course of rhodamine-actin incorporation in cardiac myocytes

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Intracellular distribution and degradation of immunoglobulin G and immunoglobulin G fragments injected into HeLa cells

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Microinjection of cultured cells using red-cell-mediated fusion and osmotic lysis of pinosomes: a review of methods and applications

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

The ubiquitin-mediated proteolytic pathway and mechanisms of energy-dependent intracellular protein degradation

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Thermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Mechanisms of Intracellular Protein Breakdown

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Rapid turnover of the histone-ubiquitin conjugate, protein A24

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Stimulation of ATP-dependent proteolysis requires ubiquitin with the COOH-terminal sequence Arg-Gly-Gly

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Protein A24 lyase activity in nucleoli of thioacetamide-treated rat liver releases histone 2A and ubiquitin from conjugated protein A24.

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Histone 2B can be modified by the attachment of ubiquitin

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Multiple ubiquitin mRNAs during xenopus laevis development contain tandem repeats of the 76 amino acid coding sequence

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Microinjection of ubiquitin: changes in protein degradation in HeLa cells subjected to heat-shock

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Ubiquitin-lysozyme conjugates. Purification and susceptibility to proteolysis

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

The human ubiquitin multigene family: some genes contain multiple directly repeated ubiquitin coding sequences

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Ubiquitin is a heat shock protein in chicken embryo fibroblasts

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Identification of ubiquitinated histones 2A and 2B in Physarum polycephalum. Disappearance of these proteins at metaphase and reappearance at anaphase.

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Intracellular protein degradation in mammalian and bacterial cells: Part 2

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Chapter 22 Red Cell-Mediated Microinjection of Macromolecules into Mammalian Cells

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Disappearance of a structural chromatin protein A24 in mitosis: implications for molecular basis of chromatin condensation

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Intracellular protein degradation

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Transfer of tRNAs to somatic cells mediated by Sendai-virus-induced fusion

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Pyridine nucleotide metabolism in mitotic cells

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Intracellular degradation of hemoglobin transferred into fibroblasts by fusion with red blood cells

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

The spatial distribution of polyribosomes in 3T3 cells and the associated assembly of proteins into the skeletal framework

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

"Covalent affinity" purification of ubiquitin-activating enzyme

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the drosophila genome

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

The yeast ubiquitin gene: head-to-tail repeats encoding a polyubiquitin precursor protein

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Protein degradation in cultured cells. The effect of fresh medium, fluoride, and iodoacetate on the digestion of cellular protein of rat fibroblasts

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Degradation rates and intracellular distributions of structurally characterized proteins injected into HeLa cells

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Conjugation of [125I]ubiquitin to cellular proteins in permeabilized mammalian cells: comparison of mitotic and interphase cells

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

What turns on heat shock genes?

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Specific recognition of calmodulin from Dictyostelium discoideum by the ATP, ubiquitin-dependent degradative pathway.

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

Microinjection of the nonhistone chromosomal protein hmg1 into bovine fibroblasts and hela cells

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2114525

High resolution acrylamide gel electrophoresis of histones

29 June 2018

1 reference

12 December 2020

The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates

1 reference

12 December 2020

Ubiquitin-lysozyme conjugates. Identification and characterization of an ATP-dependent protease from rabbit reticulocyte lysates

1 reference

12 December 2020

The degradation of guanidinated lysozyme in reticulocyte lysate

1 reference

12 December 2020

METAPHASE CHROMOSOMES AS A SOURCE OF DNA

1 reference

12 December 2020

Histone 2A, a heteromorphous family of eight protein species

1 reference

12 December 2020

Tritium labeling of proteins to high specific radioactivity by reduction methylation

1 reference

12 December 2020

Metabolism of ubiquitinated histones

1 reference

12 December 2020

The selective degradation of injected proteins occurs principally in the cytosol rather than in lysosomes

1 reference

12 December 2020

A mouse temperature-sensitive mutant defective in H1 histone phosphorylation is defective in deoxyribonucleic acid synthesis and chromosome condensation

1 reference

12 December 2020

Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins

1 reference

12 December 2020

Ubiquitin dependence of selective protein degradation demonstrated in the mammalian cell cycle mutant ts85

1 reference

12 December 2020

Functional heterogeneity of ubiquitin carrier proteins

1 reference

12 December 2020

The proteolytic inactivation of ubiquitin by rabbit liver

1 reference

12 December 2020

125I-ubiquitin is kinetically invalid as a tracer radiolabel for studies of ATP-dependent proteolysis

1 reference

12 December 2020

Identifiers

DOI

10.1083/JCB.104.3.537

1 reference

12 August 2017

1 reference

12 August 2017

PubMed publication ID

3029141

1 reference