Analysis of protein-ligand interactions by fluorescence polarization (Q35177806)

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24 January 2020

title

Analysis of protein-ligand interactions by fluorescence polarization (English)

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24 January 2020

2

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24 January 2020

author name string

Ana M Rossi

series ordinal

1

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24 January 2020

publication date

3 March 2011

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24 January 2020

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24 January 2020

volume

6

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24 January 2020

issue

3

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24 January 2020

page(s)

365-387

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Selective determinants of inositol 1,4,5-trisphosphate and adenophostin A interactions with type 1 inositol 1,4,5-trisphosphate receptors.

24 January 2020

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Binding of inositol 1,4,5-trisphosphate (IP3) and adenophostin A to the N-terminal region of the IP3 receptor: thermodynamic analysis using fluorescence polarization with a novel IP3 receptor ligand.

13 July 2018

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Synthetic partial agonists reveal key steps in IP3 receptor activation

13 July 2018

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Fluorescence lifetime and polarization-resolved imaging in cell biology

13 July 2018

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Characterization and optimization of a red-shifted fluorescence polarization ADP detection assay

13 July 2018

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Experimental approaches to evaluate the thermodynamics of protein-drug interactions.

13 July 2018

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Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules

13 July 2018

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Inositol trisphosphate receptor Ca2 release channels.

13 July 2018

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13 July 2018

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Regulation of NDR protein kinase by hydrophobic motif phosphorylation mediated by the mammalian Ste20-like kinase MST3

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Adenophostin A and analogues modified at the adenine moiety: synthesis, conformational analysis and biological activity.

13 July 2018

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Polarization of the fluorescence of macromolecules. I. Theory and experimental method

13 July 2018

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13 July 2018

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Recent advances in fluorescence correlation spectroscopy

13 July 2018

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Structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with its ligand

13 July 2018

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13 July 2018

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Surface plasmon resonance: towards an understanding of the mechanisms of biological molecular recognition

13 July 2018

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The versatility and universality of calcium signalling

13 July 2018

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Role of elementary Ca(2 ) puffs in generating repetitive Ca(2 ) oscillations

13 July 2018

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Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer

13 July 2018

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Can thermodynamic measurements of receptor binding yield information on drug affinity and efficacy?

13 July 2018

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Differential regulation of types-1 and -3 inositol trisphosphate receptors by cytosolic Ca2

13 July 2018

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Two-photon fluorescence lifetime imaging microscopy of macrophage-mediated antigen processing

13 July 2018

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Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction

13 July 2018

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Measurement of protein using bicinchoninic acid

13 July 2018

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High efficiency transformation of Escherichia coli with plasmids

13 July 2018

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

13 July 2018

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Fundamental difference between the molecular interactions of agonists and antagonists with the β-adrenergic receptor

13 July 2018

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Contribution of binding enthalpy and entropy to affinity of antagonist and agonist binding at human and guinea pig histamine H(1)-receptor

13 July 2018

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Evaluation of a fluorescence polarization assay for the detection of serum antibodies to Brucella abortus in water buffalo (Bubalus bubalis).

27 September 2018

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A fluorescence polarization-based assay for peptidyl prolyl cis/trans isomerase cyclophilin A.

27 September 2018

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High-throughput fluorescence polarization assay to identify small molecule inhibitors of BRCT domains of breast cancer gene 1.

27 September 2018

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Ligand binding energy and enzyme efficiency from reductions in protein dynamics

27 September 2018

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Development and optimization of a binding assay for the XIAP BIR3 domain using fluorescence polarization.

27 September 2018

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Fluorescence polarization assay and inhibitor design for MDM2/p53 interaction

27 September 2018

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Fluorescence polarization assays for high-throughput screening of neuropeptide FF receptors

27 September 2018

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Fluorescence anisotropy applied to biomolecular interactions.

27 September 2018

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Scintillation proximity assay

27 September 2018

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Development of a high-throughput fluorescence polarization assay for the discovery of phosphopantetheinyl transferase inhibitors

21 January 2018

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Development of a high-throughput assay to measure histidine decarboxylase activity

31 March 2020

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Adenophostins: High-Affinity Agonists of IP(3) Receptors

31 March 2020

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High efficient expression of the functional ligand binding site of the inositol 1,4,5-triphosphate receptor in Escherichia coli

31 March 2020

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Synthesis and evaluation of 1-position-modified inositol 1,4,5-trisphosphate analogs

31 March 2020

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https://api.crossref.org/works/10.1038/NPROT.2011.305

Kinetic analysis of inositol trisphosphate binding to pure inositol trisphosphate receptors using scintillation proximity assay

31 March 2020

1 reference

https://api.crossref.org/works/10.1038/NPROT.2011.305

31 March 2020

Identifiers

DOI

10.1038/NPROT.2011.305

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21372817 AND SRC:MED&resulttype=core&format=json

24 January 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21372817 AND SRC:MED&resulttype=core&format=json

24 January 2020

PubMed publication ID

21372817

1 reference