Protein engineering of subtilisin (Q34126196)

31 July 2017

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title

Protein engineering of subtilisin (English)

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31 July 2017

P N Bryan

1

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31 July 2017

language of work or name

English

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publication date

1 December 2000

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Biochimica et Biophysica Acta

31 July 2017

published in

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31 July 2017

volume

1543

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31 July 2017

issue

2

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31 July 2017

page(s)

203-222

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Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution

31 July 2017

cites work

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7 January 2021

Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain

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7 January 2021

Selection for improved subtiligases by phage display

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7 January 2021

Improvement of thermal stability of subtilisin J by changing the primary autolysis site

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7 January 2021

Designing Subtilisin BPN' To Cleave Substrates Containing Dibasic Residues

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7 January 2021

Furilisin: a variant of subtilisin BPN' engineered for cleaving tribasic substrates.

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7 January 2021

Introduction of a free cysteinyl residue at position 68 in the subtilisin Savinase, based on homology with proteinase K

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7 January 2021

Mutational replacements in subtilisin 309. Val104 has a modulating effect on the P4 substrate preference

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7 January 2021

Significance of hydrophobic S4-P4 interactions in subtilisin 309 from Bacillus lentus

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7 January 2021

Kinetic studies on the peroxidase activity of selenosubtilisin

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7 January 2021

Peroxide dependence of the semisynthetic enzyme selenosubtilisin

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7 January 2021

Mapping the active site of papain with the aid of peptide substrates and inhibitors

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7 January 2021

Crystal structure of the alkaline proteinase Savinase™ from Bacillus lentus at 1.4 Å resolution

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7 January 2021

The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis. Structural analysis, subtilisin structure and interface geometry

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7 January 2021

Incorporation of a stabilizing Ca(2 )-binding loop into subtilisin BPN'

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7 January 2021

Subtilisin BPN' variants: increased hydrolytic activity on surface-bound substrates via decreased surface activity

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7 January 2021

Energetics of folding subtilisin BPN'

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7 January 2021

Site-directed mutagenesis and the role of the oxyanion hole in subtilisin

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7 January 2021

Catalysis of a protein folding reaction: Mechanistic implications of the 2.0 .ANG. structure of the subtilisin-prodomain complex

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7 January 2021

Site-Directed Mutagenesis to Study Protein Folding and Stability

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7 January 2021

Proteases of enhanced stability: characterization of a thermostable variant of subtilisin

1 reference

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7 January 2021

Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'

1 reference

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7 January 2021

Engineering subtilisin BPN' for site-specific proteolysis.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering enzyme specificity by "substrate-assisted catalysis"

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Dissecting the catalytic triad of a serine protease

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Functional interaction among catalytic residues in subtilisin BPN'.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The 2 A crystal structure of subtilisin E with PMSF inhibitor

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The controlled introduction of multiple negative charge at single amino acid sites in subtilisin Bacillus lentus

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1 1Edited by J. Karn

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https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Site-directed mutagenesis combined with chemical modification as a strategy for altering the specificity of the S1 and S1' pockets of subtilisin Bacillus lentus

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Probing the altered specificity and catalytic properties of mutant subtilisin chemically modified at position S156C and S166C in the S1 pocket

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Toward tailoring the specificity of the S1 pocket of subtilisin B. lentus: chemical modification of mutant enzymes as a strategy for removing specificity limitations

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Electric fields in active sites: substrate switching from null to strong fields in thiol- and selenol-subtilisins

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Folding of subtilisin BPN': characterization of a folding intermediate.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Folding of subtilisin BPN': role of the pro-sequence.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering surface charges in a subtilisin: the effects on electrophoretic and ion-exchange behaviour

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Effects of engineered salt bridges on the stability of subtilisin BPN'

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Understanding and increasing protein stability

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Calcium-independent subtilisin by design

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Crystal structure analysis of subtilisin BPN' mutants engineered for studying thermal stability

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Stability of subtilisins and related proteinases (subtilases)

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Strategy and implementation of a system for protein engineering

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineered Bacillus lentus subtilisins having altered flexibility

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

A highly active and oxidation-resistant subtilisin-like enzyme produced by a combination of site-directed mutagenesis and chemical modification.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Studies of binding sites in the subtilisin from Bacillus lentus by means of site directed mutagenesis and kinetic investigations

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Interdependency of the binding subsites in subtilisin

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Reasoning enantioselectivity and kinetics of seleno-subtilisin from the subtilisin template

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

From detergent additive to semisynthetic peroxidase-simplified and up-scaled synthesis of seleno-subtilisin

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Chemical engineering of enzymes: altered catalytic activity, predictable selectivity and exceptional stability of the semisynthetic peroxidase seleno-subtilisin

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Semisynthetic Enzymes in Asymmetric Synthesis: Enantioselective Reduction of Racemic Hydroperoxides Catalyzed by Seleno-Subtilisin

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Renaturation of the mature subtilisin BPN' immobilized on agarose beads

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Increasing thermal stability of subtilisin from mutations suggested by strongly interacting side-chain clusters

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Binding rates, oxygen-sulfur substitution effects, and the pH dependence of chymotrypsin reactions

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Further evidence for the structure of the subtilisin propeptide and for its interactions with mature subtilisin.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

A covalently trapped folding intermediate of subtilisin E: spontaneous dimerization of a prosubtilisin E Ser49Cys mutant in vivo and its autoprocessing in vitro.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Improving the activity of immobilized subtilisin by site-specific attachment to surfaces

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Denaturation of subtilisin BPN′ and its derivatives in aqueous guanidine hydrochloride solutions

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Effect of the weak Ca(2 )-binding site of subtilisin J by site-directed mutagenesis on heat stability.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Identification of autoproteolytic cleavage site in the Asp-49 mutant subtilisin J by site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Scoring functions for computational algorithms applicable to the design of spiked oligonucleotides

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Cold adaptation of a mesophilic serine protease, subtilisin, by in vitro random mutagenesis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Breaking the low barrier hydrogen bond in a serine protease

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

A Weak Calcium Binding Site in Subtilisin BPN‘ Has a Dramatic Effect on Protein Stability

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Functional analysis of the intramolecular chaperone. Mutational hot spots in the subtilisin pro-peptide and a second-site suppressor mutation within the subtilisin molecule

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Display of active subtilisin 309 on phage: analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering proteases with altered specificity

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Functional analysis of the propeptide of subtilisin E as an intramolecular chaperone for protein folding. Refolding and inhibitory abilities of propeptide mutants.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The mechanism of autoprocessing of the propeptide of prosubtilisin E: intramolecular or intermolecular event?

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Binding of amino acid side-chains to S1 cavities of serine proteinases

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Interaction of subtilisin BPN' and recombinant Streptomyces subtilisin inhibitors with substituted P1 site residues.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Identification of amino acid residues responsible for the changes of absorption and fluorescence spectra on the binding of subtilisin BPN' and Streptomyces subtilisin inhibitor

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Achievement of renaturation of subtilisin BPN' by a novel procedure using organic salts and a digestible mutant of Streptomyces subtilisin inhibitor

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering subtilisin YaB: restriction of substrate specificity by the substitution of Gly124 and Gly151 with Ala.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Protein engineering of disulfide bonds in subtilisin BPN'.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Subtilisin from psychrophilic antarctic bacteria: characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The conversion of serine at the active site of subtilisin to cysteine: a "chemical mutation"

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

DNA shuffling of subgenomic sequences of subtilisin

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

A 13C-NMR study of the role of Asn-155 in stabilizing the oxyanion of a subtilisin tetrahedral adduct.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Unusual ligand binding at the active site domain of an engineered mutant of subtilisin BL.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The engineering of binding affinity at metal ion binding sites for the stabilization of proteins: subtilisin as a test case

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Structural basis of substrate specificity in the serine proteases

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Nonessential active site residues modulate selenosubtilisin's kinetic mechanism

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Kinetics of subtilisin and thiolsubtilisin

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Comparison of the kinetic specificity of subtilisin and thiolsubtilisin toward n-alkyl p-nitrophenyl esters

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The Reactivity of Thiol-subtilisin, an Enzyme Containing a Synthetic Functional Group*

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Chromatography and activity of thiol-subtilisin.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Free energy perturbation calculations on binding and catalysis after mutating Asn 155 in subtilisin

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering a novel specificity in subtilisin BPN'

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Variants of subtilisin BPN' with altered specificity profiles

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering thermostability in subtilisin BPN' by in vitro mutagenesis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Rapid folding of calcium-free subtilisin by a stabilized pro-domain mutant

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Stabilizing the subtilisin BPN' pro-domain by phage display selection: how restrictive is the amino acid code for maximum protein stability?

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Rational modification of enzyme catalysis by engineering surface charge

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering the independent folding of the subtilisin BPN' prodomain: analysis of two-state folding versus protein stability.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Thermostable variants of subtilisin selected by temperature-gradient gel electrophoresis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Preprosubtilisin Carlsberg processing and secretion is blocked after deletion of amino acids 97-101 in the mature part of the enzyme

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering new functions and altering existing functions

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Random-priming in vitro recombination: an effective tool for directed evolution

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

A pathway for conformational diversity in proteins mediated by intramolecular chaperones

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Folding mediated by an intramolecular chaperone: autoprocessing pathway of the precursor resolved via a substrate assisted catalysis mechanism

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Folding Pathway Mediated by an Intramolecular Chaperone: Characterization of the Structural Changes in Pro-subtilisin E Coincident with Autoprocess ing

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Protein memory through altered folding mediated by intramolecular chaperones.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Mutational replacements of the amino acid residues forming the hydrophobic S4 binding pocket of subtilisin 309 from Bacillus lentus

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Stereochemical modeling of disulfide bridges. Criteria for introduction into proteins by site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Prediction of electrostatic effects of engineering of protein charges.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Directed evolution of a subtilisin with calcium-independent stability

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

An engineered disulfide cross-link accelerates the refolding rate of calcium-free subtilisin by 850-fold

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Catalysis of a protein folding reaction: thermodynamic and kinetic analysis of subtilisin BPN' interactions with its propeptide fragment.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Crystal structure of selenosubtilisin at 2.0-A resolution

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering of a cold-adapted protease by sequential random mutagenesis and a screening system.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

A cold-adapted protease engineered by experimental evolution system

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The Effect of Amino Acid Deletion in Subtilisin E, Based on Structural Comparison with a Microbial Alkaline Elastase, on Its Substrate Specificity and Catalysis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Restriction of substrate specificity of subtilisin E by introduction of a side chain into a conserved glycine residue

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

The role of Pro-239 in the catalysis and heat stability of subtilisin E

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Construction of novel subtilisin E with high specificity, activity and productivity through multiple amino acid substitutions

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Random mutagenesis into the conserved Gly154 of subtilisin E: isolation and characterization of the revertant enzymes

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Thermal denaturation of streptomyces subtilisin inhibitor, subtilisin BPN', and the inhibitor-subtilisin complex.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering of S2 site of aqualysin I; alteration of P2 specificity by excluding P2 side chain

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Protein engineering of the high-alkaline serine protease PB92 from Bacillus alcalophilus: functional and structural consequences of mutation at the S4 substrate binding pocket

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Tailoring the pH dependence of enzyme catalysis using protein engineering

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Length of the acyl carbonyl bond in acyl-serine proteases correlates with reactivity

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

1 reference

7 January 2021

Evidence for intramolecular processing of prosubtilisin sequestered on a solid support

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Recombination and chimeragenesis by in vitro heteroduplex formation and in vivo repair

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Protein engineering of subtilisins to improve stability in detergent formulations

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Role of bound calcium ions in thermostable, proteolytic enzymes. Separation of intrinsic and calcium ion contributions to the kinetic thermal stability

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Engineering the independent folding of the subtilisin BPN' pro-domain: correlation of pro-domain stability with the rate of subtilisin folding

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Prodomain mutations at the subtilisin interface: correlation of binding energy and the rate of catalyzed folding

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Probing enzymic transition state hydrophobicities

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Additivity of mutational effects in proteins

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Designing substrate specificity by protein engineering of electrostatic interactions

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Details of the acyl-enzyme intermediate and the oxyanion hole in serine protease catalysis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Enzymatic catalysts in organic synthesis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

26 Enzymatic catalysis in organic synthesis

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Functional and nonfunctional mutations distinguished by random recombination of homologous genes

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Directed evolution converts subtilisin E into a functional equivalent of thermitase

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Molecular evolution by staggered extension process (StEP) in vitro recombination

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

Strategy for the directed evolution of a peptide ligase.

1 reference

https://api.crossref.org/works/10.1016/S0167-4838(00)00235-1

7 January 2021

10.1016/S0167-4838(00)00235-1

Identifiers

DOI

1 reference

31 July 2017

1 reference