A real-time high-throughput fluorescence assay for sphingosine kinases (Q33825060)

29 July 2017

title

A real-time high-throughput fluorescence assay for sphingosine kinases (English)

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29 July 2017

3

Sarah Spiegel

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29 July 2017

2

Sheldon Milstien

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29 July 2017

Santiago Lima

1

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29 July 2017

publication date

2 May 2014

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Journal of Lipid Research

29 July 2017

published in

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29 July 2017

volume

55

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29 July 2017

issue

7

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29 July 2017

page(s)

1525-1530

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Targeting the sphingosine-1-phosphate axis in cancer, inflammation and beyond

29 July 2017

cites work

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Molecular basis of sphingosine kinase 1 substrate recognition and catalysis

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Biological characterization of 3-(2-amino-ethyl)-5-[3-(4-butoxyl-phenyl)-propylidene]-thiazolidine-2,4-dione (K145) as a selective sphingosine kinase-2 inhibitor and anticancer agent

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Modulation of cellular S1P levels with a novel, potent and specific inhibitor of sphingosine kinase-1.

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Sphingosine-1-phosphate and lymphocyte egress from lymphoid organs

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Determination of sphingosine kinase 2 activity using fluorescent sphingosine by capillary electrophoresis

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

A rapid assay for assessment of sphingosine kinase inhibitors and substrates

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Sphingosine 1-phosphate and cancer

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Evaluating the utility of a bioluminescent ADP-detecting assay for lipid kinases

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

A sphingosine kinase activity assay using direct infusion electrospray ionization tandem mass spectrometry.

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Sphingosine kinase assay system with fluorescent detection in high performance liquid chromatography.

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Fluorescence-labeled sphingosines as substrates of sphingosine kinases 1 and 2.

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

Fluorescence-based assay of sphingosine kinases

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

N,N-Dimethylsphingosine is a potent competitive inhibitor of sphingosine kinase but not of protein kinase C: modulation of cellular levels of sphingosine 1-phosphate and ceramide.

4 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4076073

A rapid radioassay for sphingosine kinase

4 July 2018

1 reference

An assay for sphingosine kinase activity using biotinylated sphingosine and streptavidin-coated membranes

21 January 2018

1 reference

Sphingosine kinase: a closer look at last

21 January 2018

1 reference

Organization and Dynamics of NBD-Labeled Lipids in Membranes Analyzed by Fluorescence Recovery after Photobleaching

21 January 2018

1 reference

Synthesis of borondipyrromethene (BODIPY)-labeled sphingosine derivatives by cross-metathesis reaction

21 January 2018

1 reference

12 December 2020

High-Throughput Screening Assay for Sphingosine Kinase Inhibitors in Whole Blood Using RapidFire® Mass Spectrometry

1 reference

12 December 2020

Purification and characterization of rat kidney sphingosine kinase

1 reference

12 December 2020

Assaying sphingosine kinase activity

1 reference

12 December 2020

Identifiers

DOI

10.1194/JLR.D048132

1 reference

29 July 2017

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29 July 2017

1 reference