Transient and stable DNA transformation of Tetrahymena thermophila by electroporation (Q33741736)

29 July 2017

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title

Transient and stable DNA transformation of Tetrahymena thermophila by electroporation (English)

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29 July 2017

main subject

Tetrahymena thermophila

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Gaertig J

1

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29 July 2017

Kapler G

series ordinal

2

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29 July 2017

publication date

1 January 2000

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29 July 2017

published in

Methods in Cell Biology

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29 July 2017

volume

62

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29 July 2017

page(s)

485-500

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describes a project that uses

29 July 2017

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Transformation of Tetrahymena thermophila by electroporation and parameters effecting cell survival

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Pair formation in tetrahymena pyriformis, an inducible developmental system.

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

In Vivo Analysis of the Major Exocytosis-sensitive Phosphoprotein in Tetrahymena

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Granule lattice protein 1 (Grl1p), an acidic, calcium-binding protein in Tetrahymena thermophila dense-core secretory granules, influences granule size, shape, content organization, and release but not protein sorting or condensation

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Requirement of a small cytoplasmic RNA for the establishment of thermotolerance

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

High frequency vector-mediated transformation and gene replacement in Tetrahymena

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Electroporation-mediated replacement of a positively and negatively selectable beta-tubulin gene in Tetrahymena thermophila

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Acetylation of lysine 40 in alpha-tubulin is not essential in Tetrahymena thermophila

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Specific RNA residue interactions required for enzymatic functions of Tetrahymena telomerase

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Altering specific telomerase RNA template residues affects active site function

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

A programmed site-specific DNA rearrangement in Tetrahymena thermophila requires flanking polypurine tracts

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

A distant 10-bp sequence specifies the boundaries of a programmed DNA deletion in Tetrahymena

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Macro- and Micronuclei ofTetrahymena pyriformis: A Model System for Studying the Structure and Function of Eukaryotic Nuclei*†

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Analysis of exocytosis mutants indicates close coupling between regulated secretion and transcription activation in Tetrahymena

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in Tetrahymena thermophila

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Transformation of Tetrahymena thermophila by microinjection of a foreign gene

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Essential and nonessential histone H2A variants in Tetrahymena thermophila

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Replacement of the macronuclear ribosomal RNA genes of a mutant Tetrahymena using electroporation

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Tandem repeats of the 5' non-transcribed spacer of Tetrahymena rDNA function as high copy number autonomous replicons in the macronucleus but do not prevent rRNA gene dosage regulation

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Replication of an rRNA gene origin plasmid in the Tetrahymena thermophila macronucleus is prevented by transcription through the origin from an RNA polymerase I promoter

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

The anticodon is the signal sequence for mitochondrial import of glutamine tRNA in Tetrahymena

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Linker histones are not essential and affect chromatin condensation in vivo

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Accurate processing and amplification of cloned germ line copies of ribosomal DNA injected into developing nuclei of Tetrahymena thermophila

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Transformation of Tetrahymena to cycloheximide resistance with a ribosomal protein gene through sequence replacement

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https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Transformation of Tetrahymena thermophila with a mutated circular ribosomal DNA plasmid vector

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Amplification of tandemly repeated origin control sequences confers a replication advantage on rDNA replicons in Tetrahymena thermophila

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Circular ribosomal DNA plasmids transform Tetrahymena thermophila by homologous recombination with endogenous macronuclear ribosomal DNA

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

Constitutive Expression, Not a Particular Primary Sequence, Is the Important Feature of the H3 Replacement Variant hv2 in Tetrahymena thermophila

1 reference

https://api.crossref.org/works/10.1016/S0091-679X(08)61552-6

7 January 2021

10.1016/S0091-679X(08)61552-6

Identifiers

DOI

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