Intein applications: from protein purification and labeling to metabolic control methods (Q33652036)

28 July 2017

title

Intein applications: from protein purification and labeling to metabolic control methods (English)

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28 July 2017

David W Wood

1

1 reference

28 July 2017

Julio A Camarero

series ordinal

2

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28 July 2017

language of work or name

English

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publication date

2 April 2014

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Journal of Biological Chemistry

28 July 2017

published in

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28 July 2017

volume

289

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28 July 2017

issue

21

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28 July 2017

page(s)

14512-14519

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Protein splicing: how inteins escape from precursor proteins

28 July 2017

cites work

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions

28 July 2018

1 reference

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A redox trap to augment the intein toolbox.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Intersectional Cre driver lines generated using split-intein mediated split-Cre reconstitution

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Biological synthesis of circular polypeptides

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

In-cell fluorescence activation and labeling of proteins mediated by FRET-quenched split inteins

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Recombinant production of rhesus θ-defensin-1 (RTD-1) using a bacterial expression system.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Sequence-specific biosensors report drug-induced changes in epigenetic silencing in living cells.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Biosynthesis and antimicrobial evaluation of backbone-cyclized α-defensins

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Engineering and optimization of an allosteric biosensor protein for peroxisome proliferator-activated receptor γ ligands

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Evolution of cyclic peptide protease inhibitors

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Construction of a Bacterial Assay for Estrogen Detection Based on an Estrogen-Sensitive Intein

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Modification of transmembrane and GPI-anchored proteins on living cells by efficient protein trans-splicing using the Npu DnaE intein

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Detection of protein-protein interactions in bacteria by GFP-fragment reconstitution

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Biological Activities of Natural and Engineered Cyclotides, a Novel Molecular Scaffold for Peptide-Based Therapeutics

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Protein trans-splicing and its use in structural biology: opportunities and limitations.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Protein trans-splicing based dual-vector delivery of the coagulation factor VIII gene

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Cyclotides as templates in drug design

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Biosynthesis and biological screening of a genetically encoded library based on the cyclotide MCoTI-I.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Controllable protein cleavages through intein fragment complementation

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Split inteins as versatile tools for protein semisynthesis

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

In vivo biosynthesis of an Ala-scan library based on the cyclic peptide SFTI-1

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

"Splicing up" drug discovery. Cell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Protein ligation: applications in NMR studies of proteins.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Cyclic luciferase for real-time sensing of caspase-3 activities in living mammals

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Engineered chimeric enzymes as tools for drug discovery: generating reliable bacterial screens for the detection, discovery, and assessment of estrogen receptor modulators.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

A bacterial biosensor of endocrine modulators

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Protein splicing elements and plants: from transgene containment to protein purification

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Directed evolution of ligand dependence: small-molecule-activated protein splicing

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain.

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Rescuing a destabilized protein fold through backbone cyclization

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Protein trans-splicing to produce herbicide-resistant acetolactate synthase

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Single-column purification and bio-characterization of recombinant human parathyroid hormone-related protein (1-139).

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

High yield expression and single step purification of human thionein/metallothionein

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Production of cyclic peptides and proteins in vivo

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein

28 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

28 July 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

A method for expression and purification of soluble, active Hsp47, a collagen-specific molecular chaperone

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Simple bioseparations using self-cleaving elastin-like polypeptide tags

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Effects of thioredoxin: SUMO and intein on soluble fusion expression of an antimicrobial peptide OG2 in Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Ubiquitin-intein and SUMO2-intein fusion systems for enhanced protein production and purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Engineering a recyclable elastin-like polypeptide capturing scaffold for non-chromatographic protein purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

A dual ELP-tagged split intein system for non-chromatographic recombinant protein purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Split intein mediated ultra-rapid purification of tagless protein (SIRP).

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Biosynthesis of the cyclotide Kalata B1 by using protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Biosynthesis of a fully functional cyclotide inside living bacterial cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Intein-mediated cyclization of randomized peptides in the periplasm of Escherichia coli and their extracellular secretion

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Cyclization tag for the detection and facile purification of backbone-cyclized proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Backbone dynamics of cyclotide MCoTI-I free and complexed with trypsin.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Expressed protein ligation: a resourceful tool to study protein structure and function.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Site-specific protein cleavage in vivo by an intein-derived protease.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Bioluminescent imaging of MAPK function with intein-mediated reporter gene assay

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Detection of apoptosis using cyclic luciferase in living mammals

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Regulation of protein activity with small-molecule-controlled inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Bacterial biosensors for screening isoform-selective ligands for human thyroid receptors α-1 and β-1.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Bacterial biosensors for evaluating potential impacts of estrogenic endocrine disrupting compounds in multiple species

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Inter-chain disulfide bond improved protein trans-splicing increases plasma coagulation activity in C57BL/6 mice following portal vein FVIII gene delivery by dual vectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

In vivo, site-specific, covalent conjugation of quantum dots to proteins via split-intein splicing.

30 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4031509

Circular β-lactamase: stability enhancement by cyclizing the backbone

30 October 2018

1 reference

https://api.crossref.org/works/10.1074/JBC.R114.552653

Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor

21 January 2018

1 reference

12 December 2020

Cloning, expression, and purification of the Staphylococcus simulans lysostaphin using the intein-chitin-binding domain (CBD) system

1 reference

12 December 2020

Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein

1 reference

12 December 2020

Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments

1 reference

12 December 2020

Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803

1 reference

12 December 2020

Functional analysis of the split Synechocystis DnaE intein in plant tissues by biolistic particle bombardment

1 reference

12 December 2020

A genetic system yields self-cleaving inteins for bioseparations

1 reference

12 December 2020

Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein

1 reference

12 December 2020

Cleavage and purification of intein fusion proteins using the Streptococcus gordonii spex system

1 reference

12 December 2020

Identifiers

DOI

10.1074/JBC.R114.552653

1 reference

28 July 2017

1 reference

28 July 2017

1 reference