Quantification of AAV particle titers by infrared fluorescence scanning of coomassie-stained sodium dodecyl sulfate-polyacrylamide gels. (Q33586275)

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8 February 2020

title

Quantification of AAV particle titers by infrared fluorescence scanning of coomassie-stained sodium dodecyl sulfate-polyacrylamide gels (English)

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2

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Thomas Weber

7

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author name string

Erik Kohlbrenner

1

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Kleopatra Rapti

3

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Ronald E Gordon

4

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R Michael Linden

5

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Roger J Hajjar

6

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publication date

1 June 2012

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Human gene therapy methods

8 February 2020

published in

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volume

23

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issue

3

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page(s)

198-203

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describes a project that uses

8 February 2020

ImageJ

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11 June 2022

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based on heuristic

inferred from PubMed Central ID database lookup

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Universal real-time PCR for the detection and quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences

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Systemic errors in quantitative polymerase chain reaction titration of self-complementary adeno-associated viral vectors and improved alternative methods

21 June 2018

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The AAV vector toolkit: poised at the clinical crossroads

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Adenovirus-associated virus vector-mediated gene transfer in hemophilia B

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Neutralizing antibodies against AAV serotypes 1, 2, 6, and 9 in sera of commonly used animal models

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Calcium Upregulation by Percutaneous Administration of Gene Therapy in Cardiac Disease (CUPID): a phase 2 trial of intracoronary gene therapy of sarcoplasmic reticulum Ca2 -ATPase in patients with advanced heart failure

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Characterization of a recombinant adeno-associated virus type 2 Reference Standard Material

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Near-perfect infectivity of wild-type AAV as benchmark for infectivity of recombinant AAV vectors

21 June 2018

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Age-dependent effects of RPE65 gene therapy for Leber's congenital amaurosis: a phase 1 dose-escalation trial

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Manufacturing and characterizing AAV-based vectors for use in clinical studies

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Development of AAV serotype-specific ELISAs using novel monoclonal antibodies

21 June 2018

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Directed evolution of adeno-associated virus yields enhanced gene delivery vectors

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Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green

21 June 2018

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Quantification of adeno-associated virus particles and empty capsids by optical density measurement

21 June 2018

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Structural Proteins of Adenovirus-Associated Virus Type 3

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Synthesis of adeno-associated virus structural proteins requires both alternative mRNA splicing and alternative initiations from a single transcript.

21 June 2018

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High AAV vector purity results in serotype- and tissue-independent enhancement of transduction efficiency

21 June 2018

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Fast and reliable titration of recombinant adeno-associated virus type-2 using quantitative real-time PCR.

31 October 2018

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Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2.

31 October 2018

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Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield

31 October 2018

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Novel tools for production and purification of recombinant adenoassociated virus vectors

31 October 2018

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31 October 2018

Identifiers

DOI

10.1089/HGTB.2012.049

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:22816378 AND SRC:MED&resulttype=core&format=json

8 February 2020

1 reference

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8 February 2020

PubMed publication ID

22816378

1 reference