Optimizing the affinity and specificity of proteins with molecular display (Q33252425)

24 July 2017

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title

Optimizing the affinity and specificity of proteins with molecular display (English)

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24 July 2017

Levin AM

1

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24 July 2017

Weiss GA

series ordinal

2

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24 July 2017

publication date

8 November 2005

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24 July 2017

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24 July 2017

volume

2

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24 July 2017

issue

1

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24 July 2017

page(s)

49-57

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Tailor-made antibody therapeutics

24 July 2017

cites work

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In vitro selection as a powerful tool for the applied evolution of proteins and peptides.

21 January 2018

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Breaking the affinity ceiling for antibodies and T cell receptors

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Phage display-derived peptides as therapeutic alternatives to antibodies

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In-vitro protein evolution by ribosome display and mRNA display

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Biosynthetic polypeptide libraries

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Protein engineering by cell-surface display

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In vitro display technologies: novel developments and applications

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Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface

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Transport and anchoring of beta-lactamase to the external surface of Escherichia coli

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Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines

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Yeast surface display for screening combinatorial polypeptide libraries

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An in vitro polysome display system for identifying ligands from very large peptide libraries

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RNA-peptide fusions for the in vitro selection of peptides and proteins

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STABLE: protein-DNA fusion system for screening of combinatorial protein libraries in vitro

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cDNA - protein fusions: covalent protein - gene conjugates for the in vitro selection of peptides and proteins

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Selection of phage antibodies by binding affinity

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Affinity maturation of human growth hormone by monovalent phage display

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Growth hormone binding affinity for its receptor surpasses the requirements for cellular activity

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Structural and mutational analysis of affinity-inert contact residues at the growth hormone-receptor interface

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Affinity maturation of leukemia inhibitory factor and conversion to potent antagonists of signaling.

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A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter

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Dissecting the Engrailed homeodomain-DNA interaction by phage-displayed shotgun scanning

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Additivity of mutational effects in proteins

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Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments

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Directed evolution of a protein: selection of potent neutrophil elastase inhibitors displayed on M13 fusion phage

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Rapid evolution of peptide and protein binding properties in vitro

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Selecting high-affinity binding proteins by monovalent phage display

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In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence

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A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites

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Building zinc fingers by selection: toward a therapeutic application

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The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF

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Comprehensive functional maps of the antigen-binding site of an anti-ErbB2 antibody obtained with shotgun scanning mutagenesis

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EF-Tu binding peptides identified, dissected, and affinity optimized by phage display

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In vitro selection from protein and peptide libraries

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Combinatorial evolution of high-affinity peptides that bind to the Thomsen-Friedenreich carcinoma antigen

21 January 2018

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Identification of peptide sequences that bind the Thomsen-Friedenreich cancer-associated glycoantigen from bacteriophage peptide display libraries

21 January 2018

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Characterization of peptides that bind the tumor-associated Thomsen-Friedenreich antigen selected from bacteriophage display libraries

21 January 2018

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Discovery of high-affinity peptide binders to BLyS by phage display

21 January 2018

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Stable, soluble T-cell receptor molecules for crystallization and therapeutics

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Directed evolution of human T-cell receptors with picomolar affinities by phage display

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Development of humanized antibodies as cancer therapeutics

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Phage antibodies: filamentous phage displaying antibody variable domains

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Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site

21 January 2018

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Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection

21 January 2018

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Tailoring in vitro selection for a picomolar affinity human antibody directed against vascular endothelial growth factor receptor 2 for enhanced neutralizing activity

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High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold

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In vitro antibody evolution targeting germline hot spots to increase activity of an anti-CD22 immunotoxin

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Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity

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Directed evolution, phage display and combination of evolved mutants: a strategy to recover the neutralization properties of the scFv version of BCF2 a neutralizing monoclonal antibody specific to scorpion toxin Cn2.

21 January 2018

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A strategy for the generation of specific human antibodies by directed evolution and phage display. An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom

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Molecular characterization of the circulating anti-HIV-1 gp120-specific B cell repertoire using antibody phage display libraries generated from pre-selected HIV-1 gp120 binding PBLs.

21 January 2018

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Fine tuning of the specificity of an anti-progesterone antibody by first and second sphere residue engineering

21 January 2018

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Changes in the specificity of antibodies against steroid antigens by introduction of mutations into complementarity-determining regions of the V(H) domain.

21 January 2018

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Expanding the conformational diversity by random insertions to CDRH2 results in improved anti-estradiol antibodies

21 January 2018

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Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis

21 January 2018

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Engineering the steroid-specificity of an anti-17beta-estradiol Fab by random mutagenesis and competitive phage panning

21 January 2018

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In vitro scanning saturation mutagenesis of an antibody binding pocket

21 January 2018

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Practical applications of engineering gram-negative bacterial cell surfaces

21 January 2018

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Antibody affinity maturation using bacterial surface display.

21 January 2018

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Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface.

21 January 2018

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Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies

21 January 2018

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Rapid isolation of high-affinity protein binding peptides using bacterial display

21 January 2018

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Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library

21 January 2018

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Isolation of anti-T cell receptor scFv mutants by yeast surface display

21 January 2018

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Optimal screening of surface-displayed polypeptide libraries

21 January 2018

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In vitro evolution of a T cell receptor with high affinity for peptide/MHC.

21 January 2018

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High affinity T cell receptors from yeast display libraries block T cell activation by superantigens.

21 January 2018

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TCRs with high affinity for foreign pMHC show self-reactivity

21 January 2018

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High-affinity, peptide-specific T cell receptors can be generated by mutations in CDR1, CDR2 or CDR3.

21 January 2018

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Interleukin-2 mutants with enhanced alpha-receptor subunit binding affinity

21 January 2018

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Yeast mating for combinatorial Fab library generation and surface display

21 January 2018

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Affinity maturation of Fab antibody fragments by fluorescent-activated cell sorting of yeast-displayed libraries

21 January 2018

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Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity

21 January 2018

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Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody

21 January 2018

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Directed evolution of an anti-carcinoembryonic antigen scFv with a 4-day monovalent dissociation half-time at 37 degrees C.

21 January 2018

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A general method for greatly improving the affinity of antibodies by using combinatorial libraries

21 January 2018

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Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody

21 January 2018

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Development of a human light chain variable domain (V(L)) intracellular antibody specific for the amino terminus of huntingtin via yeast surface display

21 January 2018

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Ribosome display: an in vitro method for selection and evolution of antibodies from libraries

21 January 2018

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In vitro selection and evolution of functional proteins by using ribosome display

21 January 2018

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Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries.

21 January 2018

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An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues

21 January 2018

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Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

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Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display

21 January 2018

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Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity

21 January 2018

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Engineered proteins as specific binding reagents.

21 January 2018

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High-affinity binders selected from designed ankyrin repeat protein libraries

21 January 2018

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In vitro virus: Bonding of mRNA bearing puromycin at the 3′‐terminal end to the C‐terminal end of its encoded protein on the ribosome in vitro

21 January 2018

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mRNA display: ligand discovery, interaction analysis and beyond

21 January 2018

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The use of mRNA display to select high-affinity protein-binding peptides

21 January 2018

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Functional proteins from a random-sequence library

21 January 2018

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Directed evolution of high-affinity antibody mimics using mRNA display

21 January 2018

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Fine affinity discrimination by yeast surface display and flow cytometry

21 January 2018

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Bacterial surface presentation of proteins and peptides: an alternative to phage technology?

21 January 2018

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21 January 2018

Identifiers

DOI

10.1039/B511782H

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24 July 2017

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