Directed evolution of enzyme catalysts (Q28258199)

Tolerance of different proteins for amino acid diversity

Europe PubMed Central

title

Directed evolution of enzyme catalysts (English)

0 references

0 references

O Kuchner

1

0 references

F H Arnold

series ordinal

2

0 references

language of work or name

English

0 references

publication date

December 1997

0 references

published in

Trends in Biotechnology

0 references

volume

15

0 references

issue

12

0 references

page(s)

523-30

0 references

cites work

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Directed evolution: Creating biocatalysts for the future

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Rapid evolution of a protein in vitro by DNA shuffling

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Directed evolution of a subtilisin with calcium-independent stability

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Molecular evolution of an arsenate detoxification pathway by DNA shuffling.

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Development of an in vivo method to identify mutants of phage T4 lysozyme of enhanced thermostability

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Increase in thermostability of recombinant barley beta-amylase by random mutagenesis

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Proteases of enhanced stability: characterization of a thermostable variant of subtilisin

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Substitutions of Ser for Asn-163 and Pro for Leu-264 are important for stabilization of lipase from Pseudomonas aeruginosa

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Polymerase chain reaction-based random mutagenesis: production and characterization of thermostable mutants of Zymomonas mobilis alcohol dehydrogenase-2.

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Additivity of mutational effects in proteins

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Hyperthermostable variants of a highly thermostable alpha-amylase

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Screening of stable proteins in an extreme thermophile, Thermus thermophilus.

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Combinatorial protein design: strategies for screening protein libraries

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Cold adaptation of a mesophilic serine protease, subtilisin, by in vitro random mutagenesis

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

From adenylate cyclase to guanylate cyclase

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Guided evolution of enzymes with new substrate specificities

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Selection of Streptomyces griseus protease B mutants with desired alterations in primary specificity using a library screening strategy

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Conversion from farnesyl diphosphate synthase to geranylgeranyl diphosphate synthase by random chemical mutagenesis.

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

A mutant T7 RNA polymerase as a DNA polymerase.

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Glutathione Transferases with Novel Active Sites Isolated by Phage Display from a Library of Random Mutants

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Hydrolytic antibodies: variations on a theme

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotides

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Cloning of random-sequence oligodeoxynucleotides

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Context dependence of phenotype prediction and diversity in combinatorial mutagenesis

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Optimization of DNA shuffling for high fidelity recombination

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Random recombination of antibody single chain Fv sequences after fragmentation with DNasel in the presence of Mn2

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

3D structural information as a guide to protein engineering using genetic selection

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021

Fitness spectrum among random mutants on Mt. Fuji-type fitness landscape

1 reference

https://api.crossref.org/works/10.1016/S0167-7799(97)01138-4

7 January 2021