Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation (Q27681624)

14

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Emmanuelle Charpentier

13

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David W Taylor

David W Taylor

3

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Martin Jínek

Martin Jinek

1

0 references

Kaihong Zhou

9

Kaihong Zhou

0 references

Michael Hauer

Michael Hauer

8

1 reference

ORCID Public Data File 2021

Samuel H Sternberg

4

Samuel H Sternberg

0 references

Fuguo Jiang

2

Fuguo Jiang

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author name string

Emine Kaya

5

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Enbo Ma

6

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Carolin Anders

7

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Steven Lin

10

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Matias Kaplan

11

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Anthony T Iavarone

on focus list of Wikimedia project

12

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language of work or name

English

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publication date

14 March 2014

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published in

Science

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volume

343

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issue

6176

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page(s)

1247997

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ScienceSource

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cites work

Cas9 as a versatile tool for engineering biology

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Crystal structure of RuvC resolvase in complex with Holliday junction substrate

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

RNA-guided human genome engineering via Cas9

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Multiplex genome engineering using CRISPR/Cas systems

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Mechanism of Foreign DNA Selection in a Bacterial Adaptive Immune System

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

CRISPR-based adaptive immune systems

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Structure of DNMT1-DNA Complex Reveals a Role for Autoinhibition in Maintenance DNA Methylation

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Encounter and extrusion of an intrahelical lesion by a DNA repair enzyme

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Crystal structures of DNA/RNA repair enzymes AlkB and ABH2 bound to dsDNA

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Electrostatics of nanosystems: application to microtubules and the ribosome

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Structure and mechanism of DNA topoisomerase II

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Towards automated crystallographic structure refinement with phenix.refine

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

ConSurf 2010: calculating evolutionary conservation in sequence and structure of proteins and nucleic acids

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Automated structure solution with autoSHARP

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

EMAN2: an extensible image processing suite for electron microscopy

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Scaling and assessment of data quality

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Coot: model-building tools for molecular graphics

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Refinement of macromolecular structures by the maximum-likelihood method

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

SOLVE and RESOLVE: automated structure solution, density modification and model building

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

RNA-programmed genome editing in human cells

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Small CRISPR RNAs guide antiviral defense in prokaryotes

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

DNA binding and cleavage by the HNH homing endonuclease I-HmuI

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense

20 March 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

PHENIX: a comprehensive Python-based system for macromolecular structure solution

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Features and development of Coot

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

MolProbity: all-atom contacts and structure validation for proteins and nucleic acids

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Proposal for a common nomenclature for sequence ions in mass spectra of peptides

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Automated structure solution with the PHENIX suite

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Phasercrystallographic software

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

A new generation of the IMAGIC image processing system

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

EMAN: semiautomated software for high-resolution single-particle reconstructions

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

UCSF Chimera--a visualization system for exploratory research and analysis

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

ESPript: analysis of multiple sequence alignments in PostScript

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Dali: a network tool for protein structure comparison

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Clustal W and Clustal X version 2.0

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Substructure solution with SHELXD

22 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

22 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

RNA-guided genetic silencing systems in bacteria and archaea

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Evolution and classification of the CRISPR-Cas systems

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Making and breaking nucleic acids: two-Mg2 -ion catalysis and substrate specificity

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Automated molecular microscopy: the new Leginon system

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Appion: an integrated, database-driven pipeline to facilitate EM image processing

7 April 2017

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

22 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Structure of an RNA silencing complex of the CRISPR-Cas immune system.

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Autoindexing diffraction images with iMosflm

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

A CRISPR/Cas system mediates bacterial innate immune evasion and virulence

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

CRISPR-mediated adaptive immune systems in bacteria and archaea

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Tuning in to interference: R-loops and cascade complexes in CRISPR immunity.

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Enforced presentation of an extrahelical guanine to the lesion recognition pocket of human 8-oxoguanine glycosylase, hOGG1.

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Structures of the RNA-guided surveillance complex from a bacterial immune system

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Clustered regularly interspaced short palindromic repeats (CRISPRs): the hallmark of an ingenious antiviral defense mechanism in prokaryotes

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Nucleases: diversity of structure, function and mechanism.

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

An equivalent metal ion in one- and two-metal-ion catalysis

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

SPARX, a new environment for Cryo-EM image processing

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli

29 September 2017

2 references

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

29 September 2017

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Structure of the CRISPR interference complex CSM reveals key similarities with cascade

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus.

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4184034

Analysis of CRISPR system function in plant pathogen Xanthomonas oryzae.

2 June 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

ACE: automated CTF estimation

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

FindEM--a fast, efficient program for automatic selection of particles from electron micrographs

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Image processing for electron microscopy single-particle analysis using XMIPP.

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy.

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Using situs for flexible and rigid-body fitting of multiresolution single-molecule data

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

Multi-resolution contour-based fitting of macromolecular structures

21 January 2018

1 reference

https://api.crossref.org/works/10.1126/SCIENCE.1247997

MAFFT: iterative refinement and additional methods

21 January 2018

1 reference

9 October 2022

https://opencitations.net/index/coci/api/v1/references/10.1126/SCIENCE.1247997

Identifiers

DOI

10.1126/SCIENCE.1247997

1 reference

1 reference

1 reference

PubMed publication ID

24505130

1 reference