Prime Medicine, Inc.’s Post

https://lnkd.in/dK5pDFYG "Credit: AI-generated image Scientists at the Broad Institute of MIT and Harvard have improved a gene-editing technology that is now capable of inserting or substituting entire genes in the genome in human cells efficiently enough to be potentially useful for therapeutic applications." "More information: Pandey S, Gao XD, et al. Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing, Nature Biomedical Engineering (2024). DOI: 10.1038/s41551-024-01227-1 Journal information: Nature Biomedical Engineering "

Membrane-derived particles, wrapped in membrane fragments, have been engineered to transport CRISPR genome editing tools to target cells within the body for intricate genome engineering. Led by Nobel laureate Jennifer Doudna, researchers have developed enveloped delivery vehicles (EDVs) capable of ferrying Cas9 and gRNA proteins, enabling precise genome editing within living organisms. This breakthrough paves the way for a versatile and programmable delivery approach with promising therapeutic applications. https://lnkd.in/eAYbvZMu

𝐄𝐱𝐩𝐞𝐫𝐢𝐞𝐧𝐜𝐞 𝐭𝐡𝐞 𝐩𝐨𝐰𝐞𝐫 𝐨𝐟 𝐆𝐞𝐧𝐨𝐭𝐲𝐩𝐢𝐧𝐠 & 𝐏𝐡𝐞𝐧𝐨𝐭𝐲𝐩𝐢𝐧𝐠! Co-localize proteins, mRNA, DNA, and miRNA in one tissue section, revealing profound insights into cellular processes. Explore the intricate world of cellular dynamics with our latest blog! Unveil the secrets of cellular functions with our latest blog on Genotyping & Phenotyping technology! Witness the co-localization of key biomolecules in a single tissue section, shedding light on cellular intricacies. Dive deep into cutting-edge research! 𝐋𝐞𝐚𝐫𝐧 𝐌𝐨𝐫𝐞: https://hubs.la/Q02j-Sn20 #ResearchRevelations #Genotyping #Phenotyping #CellularProcesses

The LightON mRNA💡🧬mRNA Biology Masterclass returns, in partnership with Endpoints News! This edition will explore the pivotal role that generating and analyzing large-scale mRNA biology data plays, providing the necessary foundation for leveraging AI technology. 📅 Join us on March 12th to hear from our speakers, Iris Alroy, Co-founder and CSO of Anima Biotech, and Gene Yeo, Professor of Cellular & Molecular Medicine at the University of California, as they delve into how AI can be applied to revolutionize our understanding of the complex mechanisms underlying mRNA regulation. 👉Register Today http://bit.ly/3SBCfjf #mRNABiology #ai #genomics #masterclass

𝗥𝗲𝘁𝗿𝗼𝗻 𝗔𝗿𝗿𝗮𝘆𝘀 𝗘𝗻𝗮𝗯𝗹𝗲 𝗠𝘂𝗹𝘁𝗶-𝗦𝗶𝘁𝗲 𝗚𝗲𝗻𝗼𝗺𝗲 𝗘𝗱𝗶𝘁𝗶𝗻𝗴 In a recent study, scientists introduced a new method for precise, simultaneous multi-site genome editing using retron arrays. The so-called multitron architecture is compatible with CRISPR editing in human cells. American researchers have developed the multitron system, leveraging bacterial retrons to produce multiple editing donors from a single transcript. This technology allows for precise, concurrent modifications at multiple genomic sites in both prokaryotic and eukaryotic cells. The approach was validated through molecular recording, genetic element minimisation, and metabolic engineering applications. The multitron system uses arrays of retron-derived donor DNA to edit multiple genome sites simultaneously. This contrasts with traditional methods that typically involve laborious cycles of single-site edits. The study demonstrated the method’s efficacy in bacteria, editing up to five target sites with varying efficiencies. Read on at CRISPR Medicine News to learn how the multitron system performed in human cells👇 https://lnkd.in/dyJGVzKB #crisprmedicinenews #crisprmedicine #crispr #geneediting Seth Shipman Gladstone Institutes University of California, San Francisco

🌟 So excited for this webinar! It's the first to delve into one of the most crucial aspects of our mRNA Biology-AI series: proprietary large-scale data. A huge thanks to our speakers and our partner Endpoints News. 🙌 Looking forward to bringing valuable insights to the scientific community. 💡

Very proud to introduce our MEGA system: a multifunctional, high-resolution, genomic "Swiss Army Knife". MEGA is capable of creating single point mutations, DSBs, and cytosine demethylation to recapitulate the full function of AID in genomic and epigenomic editing. Key Highlights: - First-of-its-kind modular AID-based editor that embodies the full spectrum of genomic and epigenomic editing activity. - Better understanding of AID's role in (epi)genomic reprogramming and its complex catalytic activities. - "Swiss Army Knife" editing toolbox that paves the way for a significant advancement in genomic and epigenomic editing for basic and medical applications including monoclonal antibody development. The paper was led by Julian Weischedel, PhD with the help of several colleagues including Dr Tom MacCarthy; we dedicate this work to his memory. To read more: https://lnkd.in/erG557id #GeneEditing #AdaptiveImmunity #Cas9 #AID #Research #Science #Innovation #NucleicAcidsResearch

Multiome Accessibility Gene Integration Calling and Looping (MAGICAL) addressed the unmet need to identify differential regulatory circuits based on single-cell multiomics data from contrast conditions. Critically, it identifies regulatory circuits involving distal chromatin sites. The previously difficult-to-predict distal regulatory sites are increasingly recognized as key for understanding gene regulatory mechanisms p/b Princeton University, Icahn School of Medicine at Mount Sinai, Duke University, Yale University, Battelle, Weill Cornell Medicine, Yale University School of Medicine, Naval Medical Research Center, Antonio Cappuccio, Frédérique Ruf-Zamojski, Ph.D., Aliza Rubenstein, PhD, German Nudelman, Alicja Tadych (Matusewicz), Alexandria Vornholt, Mary Catherine George, Felicia Ruffin, Michael Dagher, Daniel Chawla, Alessandra Soares Schanoski, Rachel Spurbeck, PhD, Robert Sebra, Steven Kleinstein, Andrew Letizia, Irene Ramos Lopez, Christopher Woods, Elena Zaslavsky, Olga Troyanskaya, Stuart Sealfon #multiomics #dataanalytics #translationalresearch #regulation