PacBio’s Post

🌞🔬 We're back from the holiday weekend and reflecting on the standout publications from June. 🚀 From uncovering the secrets of single-cell RNA-seq to decoding human segmental duplications, PacBio technology is at the forefront of scientific discovery. Highlights include: • 𝐒𝐢𝐧𝐠𝐥𝐞-𝐜𝐞𝐥𝐥 𝐑𝐍𝐀-𝐬𝐞𝐪: More consistent gene expression profiles and novel isoform discovery. • 𝐏𝐨𝐩𝐮𝐥𝐚𝐭𝐢𝐨𝐧 𝐬𝐞𝐪𝐮𝐞𝐧𝐜𝐢𝐧𝐠: Unraveling human segmental duplications and genetic diversity. • 𝐆𝐞𝐧𝐞 𝐫𝐞𝐠𝐮𝐥𝐚𝐭𝐢𝐨𝐧: Haplotype-resolved views of chromatin accessibility. • 𝐌𝐞𝐭𝐚𝐠𝐞𝐧𝐨𝐦𝐢𝐜𝐬: High-quality, species-level resolution of microbial communities. Jump into the latest Powered by PacBio blog to read more: https://bit.ly/4eQuC1v #PacBio #Genomics #HiFiSequencing

🧬✨ Unveiling the Genomic Frontier! ✨🧬 Happy that my latest article is Published on Medium tilted: "Human Genome Project: From Blueprint to Revolution." 🌍🔍 Step into the world where science meets innovation as I uncover how the Human Genome Project is reshaping healthcare and biotech landscapes. From unlocking the secrets of our genetic blueprint to paving the way for personalized medicine, this journey is nothing short of revolutionary! 📖 Explore the full article here:  https://lnkd.in/g3YweG6D #humangenome #genetics #biotechnology #humangenomeproject #mediumarticle

The Human Genome Project (HGP), completed in 2003, was a groundbreaking international scientific effort that sequenced the human genome and mapped all human genes. Key outcomes included: Sequencing 92% of the human genome initially, with a gapless assembly completed in 2022. Accelerating human biology research and improving medical practices. Advancing DNA sequencing technologies and promoting open data sharing. Laying the foundation for personalized medicine. Personalized medicine has since evolved beyond genetics to include factors such as microbiome, environment, lifestyle, and broader health data integration. Recent developments in non-genetic biomarkers for disease prediction focus on microRNAs, blood-based protein biomarkers, metabolomics, inflammatory proteins, gene expression profiles, and circulating tumor DNA. These advancements reflect a trend towards more comprehensive, integrated approaches to personalized medicine and biomarker discovery, aiming to capture the complex interplay of genetic and non-genetic factors in health and disease.

Will we eventually be able to engineer our microbiomes to achieve better health outcomes? This article takes a look at some interesting work happening to targeting pathogens and beneficial microbes in the gut. Scientists have genetically modified isolated microbes for decades. Now, using CRISPR, they intend to target entire microbiomes. #microbiome #microbiology

🧬Researchers from Hiroshima University have developed a groundbreaking software tool known as DANGER analysis, offering a transformative solution for safer and more precise genome editing in all organisms with a transcriptome. 💬"Our DANGER analysis is a novel software that enables quantifying phenotypic effects caused by estimated off-target. Furthermore, our tool uses de novo transcriptome assembly whose sequences can be built from RNA-sequencing data of treated samples without a reference genome." - Dr. Hidemasa Bono, Genome Editing Innovation Center 📍 With its ability to identify on- and off-target sites, evaluate phenotypic effects, and quantify phenotypic risk without reliance on a reference genome, DANGER analysis opens new frontiers in medicine, agriculture, and biological research. Follow the link to learn more: https://lnkd.in/e2y3hNHQ #CRISPR #GenomeEditing #GeneEditing #GeneticResearch #Genetics #Genome #LifeSciences #LifeSciencesNews

Genome editing has become a widely adopted technology for modifying DNA in cells, enabling scientists to develop therapies to repair disease-causing mutations and study diseases in the lab. However, current methods allow for editing only one location at a time. A team of scientists has now developed a new method that allows precise edits in multiple locations within a cell simultaneously. By using molecules called retrons, they created a tool that can efficiently modify DNA in bacteria, yeast, and human cells. #Genome #Health #Genetics #Science González-Delgado, A., Lopez, S.C., Rojas-Montero, M. et al. Simultaneous multi-site editing of individual genomes using retron arrays. Nat Chem Biol (2024). https://lnkd.in/efQZv3_f

It’s exciting to see a new milestone in genetic engineering being achieved by the Synthetic Yeast Genome Project, an international research group unaffiliated with Anthony Nolan, who have just produced a strain of yeast with a genome of more than 50% synthetic DNA. Although cells with fully synthetic DNA have previously been engineered in the lab, they often function abnormally and usually only involve simple genomes. In contrast, standard brewer’s yeast (Saccharomyces cerevisiae) has 16 chromosomes – and the Synthetic Yeast Genome Project are aiming to engineer the first eukaryote with a fully synthetic genome. This new milestone of achieving a genome with over 50% synthetic DNA validates the team’s approach, whereby strains of yeast are created each with a single edited chromosome, and bred together to eventually create an organism with 7.5 synthetic chromosomes. This part-synthetic yeast was able to survive and replicate, a huge achievement. Results like these are exciting as they advance our understanding of what is possible with genetic engineering, and could open up new avenues for research into cell and gene therapies. #cellandgenetherapy https://lnkd.in/e8hXZiEy 

MIT scientists have identified thousands of programmable DNA-cutting enzymes called Fanzors in a range of organisms, from snails to algae. Fanzors are RNA-guided enzymes that can be used for precise DNA cutting, similar to CRISPR. This discovery enhances the toolbox for genetic research and medical applications. Notably, Fanzors were the first of their kind found in eukaryotic organisms, offering potential benefits in safe and efficient genome editing. Researchers aim to develop advanced genome editing tools from Fanzors and explore their capabilities in the field. Read more: https://lnkd.in/dgYJ9XQd #dna #rnaediting #biotechnology #geneticresearch #biochemistry

𝗠𝗮𝗽𝗽𝗶𝗻𝗴 𝗪𝗵𝗲𝗮𝘁 𝗥𝗲𝗽𝗿𝗼𝗱𝘂𝗰𝘁𝗶𝘃𝗲 𝗗𝗲𝘃𝗲𝗹𝗼𝗽𝗺𝗲𝗻𝘁 𝘄𝗶𝘁𝗵 𝗦𝗽𝗮𝘁𝗶𝗮𝗹 𝗧𝗿𝗮𝗻𝘀𝗰𝗿𝗶𝗽𝘁𝗼𝗺𝗶𝗰𝘀 How is #SpatialTranscriptomics transforming our understanding of gene expression in plants? At #EISingleCell24, PhD Researcher Katie Long from the John Innes Centre will be sharing her work looking at the expression patterns of 300 key genes in developing wheat spikes. Katie is currently working in collaboration with Ashleigh Lister in EI's single-cell labs, using our #VizgenMERSCOPE platform which performs highly multiplexed, spatially resolved measurements of gene expression. You can view the full line up of speakers and register below: https://okt.to/xVLHGi

A team of researchers has developed a software tool called DANGER (Deleterious and ANticipatable Guides Evaluated by RNA-sequencing) analysis that provides a way for the safer design of genome editing in all organisms with a transcriptome. For about a decade, researchers have used the CRISPR technology for genome editing. However, there are some challenges in the use of CRISPR. The DANGER analysis overcomes these challenges and allows researchers to perform safer on- and off-target assessments without a reference genome. It holds the potential for applications in medicine, agriculture, and biological research. #artificialintelligence #genomics #genomeediting https://lnkd.in/dbcuxy5k