Frontiers in Bioscience-Landmark’s Post

The misfolding of cellular proteins is responsible for ageing.  The intrinsically disordered regions (IDRs) are present in those proteins that interact with biomolecules by induced fit. Researchers have found that peptidyl-prolyl isomerase A (PPIA) acts as a chaperone (those proteins that help in proper folding of misfolded proteins) of proteins containing the IDRs. It was observed that there were reduced levels of PPIA in haematopoietic stem cells (HSCs) of older mice compared to that of younger mice. It was concluded that the PPIA is essential for the translation of proteins with IDRs, & the reduced amounts of PPIA in HSCs is an indicator of cellular ageing. #biotech #biotechnology #cell #chaperone #ageing https://lnkd.in/guMZx45s

https://lnkd.in/ehheZNTe Article title: Experimental in vitro modeling of hematopoiesis by using diffusion chambers of an original design Author(s): Denys Bilko Journal: Annals of Bone Marrow Research   Journal ISSN: 2692-4684 Abstract: One of the most important tasks of biotechnology and regenerative medicine is to achieve long-term expansion of hematopoietic stem cells. One of the promising approaches to optimizing the cultures of hematopoietic cells is the creation of a microenvironment in the form of feeder stromal layers, which allows for the reproduction of the combination of soluble cytokines and growth factors necessary for hematopoiesis and promotes an increase in their proliferation [1, 2]. However, there is always a risk of contamination of the future graft by feeder cells. Therefore, the purpose of the work was to create a model for supporting hematopoiesis in soft hydrogel diffusion chambers immersed in a nutrient medium with a feeder layer and to determine whether direct contact between the hematopoietic cells and the feeder is necessary for full support of hematopoiesis in vitro. To realize this goal (see Figure 1), the method of culturing in gel diffusion chambers in vitro, clonogenic assay in semiliquid agar, cytological, immunohistochemical methods, and methods of variation statistics were used. #Cultureinvitro #Hematopoiesis #Hematopoieticstemcell #Hematopoieticcellexpansion #Geldiffusionchambers #Feederlayers #BoneMarrow #BloodSamples #Hematology #AutoimmuneDiseases #StemCells #Immunology #Transplantation #MinimalInvasiveSurgeryTechniques #TranscriptionFactors #Medication #TropicalTreatments #Metabolism #Mortality #DrugTargeting #DrugSensitivity #Resistance #HabitsAndLifestyle #PrognosticFactors #Pathophysiology #NovelDrugs #CellularTherapy #TranslationalResearch #MesenchymalStemCell #HematopoieticStemCellTransplantation #BoneMarrowTransplantation #BoneMarrowEdema #BoneMarrowCancer #BoneMarrowBarrier

A very interesting paper describing the current state of organoids, which are three-dimensional cell cultures derived from stem cells that closely mimic the architecture and functionality of native organs. Biospecimens are a really important part of drug discovery because it's from tissue samples that you get the molecular data that will help provide you more insights about what drug targets might be worth considering. For some therapeutic areas of drug discovery, the availability of tissues can be challenging. In some cases, it can be impractical to extract, collect and process tissue samples from patients. It will be interesting to see whether the use of grown organoids can help provide researchers with good tissue samples more easily provide useful specimens that can shorten pre-clinical activities. From a data perspective, it will be interesting to learn what kind of metadata about organoids would help inform data acquisition decisions for precision medicine groups. #organoid #precisionmedicine https://lnkd.in/e57Hn2mP

A fascinating study of Extracellular Vesicle (EV) production in Vertical-Wheel bioreactor! hMSCs were expanded in a 3D Synthemax II microcarrier-based system using a PBS mini 0.1L Vertical-Wheel bioreactor. The bioreactor, operating under variable shear stress levels, significantly increased extracellular vesicle (EV) secretion from hMSCs by 2.5-fold compared to static 2D culture. Notably, the bioreactor altered the microRNA cargo of the EVs, upregulating specific miRNAs. Proteomics analysis revealed an upregulation of metabolic, autophagy, and ROS-related proteins in EVs from the bioreactor. The scalability of the Vertical-Wheel bioreactor system was also demonstrated in a 0.5L bioreactor, showcasing its potential for stem cell-derived EV bio-manufacturing with implications for cell-free therapy in neurological disorders.

Unlock the full potential of your iPSC research with ChemoMetec's automated cell counting technology 🧪✨   Induced pluripotent stem cells (iPSCs) are transforming regenerative medicine and drug discovery. Precision and reliability in cell counting are crucial for advancing your iPSC applications.   As iPSCs differentiate, they tend to form aggregates. In order to monitor these cells as they grow, precise and accurate measurements of cell number and cell viability are needed. Using the NucleoCounter® NC-202™, any aggregated cell type can be counted reproducibly using the aggregated cell count assay, enabling greater process control and understanding.   Elevate your stem cell research and accelerate breakthroughs. Learn more about how ChemoMetec can support your iPSC projects.   #iPSC #StemCells #ChemoMetec #CellCounting #CellAnalysis #Lifescience

PUBLICATION 📚| Liver #spheroids grown in 500 µm microwells #Gri3D plates to obtain 73 microtissues per well. 🔬 Scientists from The University of Edinburgh describe how to produce human #liverspheres in our plate #Gri3D, providing a powerful tool to generate human liver tissue for #diseasemodeling and #drugscreening. This system harnessed the self-assembly potential of pluripotent stem cell-derived hepatic progenitors, endothelial cells, and hepatic stellate cells to grow homogenous hepatic 3D microtissues in an #automated and #highthroughput manner. 📄 Have a look at the protocol here: https://lnkd.in/gK6Mcwkh and contact us ([email protected]) if you want to learn more! #3Dcellculture #organoids #publication #biotechnology #research #invitromodel

Incredible work! Organoids research has led to the creation of a physiologically relevant in vitro model of the human bone marrow microenvironment. Hematopoietic development and diseases can now be studied more closely using SCTi003-A iPSC line. Microenvironment modeling in this manner allows for a better understanding of cellular, structural, and molecular characteristics of hematopoiesis. Generation of complex bone marrow organoids from human induced pluripotent stem cells ➡ https://lnkd.in/duasDtuh

Converging trends in cell manufacturing, synthetic biology, and bioinformatics are today transforming engineered #mitochondria transplantation therapies from mere theory into practice. Mesenchymal stromal / #stemcells are sure to play a pivotal role in this exemplar application for "MSC 2.0". 📘 Learn more here: https://lnkd.in/ebRkczvy #celltherapymanufacturing #syntheticbiology #cellbiology #geneticengineering #bioinformatics #celltherapy

BEAU-TI-FUL WORK! Researchers at Ludwig-Maximilians-Universität München and collaborators in Germany, Austria, Japan, and Canada created a physiologically relevant in vitro model of the human bone marrow (BM) microenvironment, which will allow studying hematopoietic development and BM diseases more closely. STEMCELL Technologies' SCTi003-A - a highly quality controlled iPSC line - was one of the lines used to generate key cell types that self-organize into spatially defined 3D structures mimicking cellular, structural and molecular characteristics of the hematopoietic microenvironment. Generation of complex bone marrow organoids from human induced pluripotent stem cells ➡ https://lnkd.in/duasDtuh #organoids #ipsc #stemcell #stemcellresearch #bonemarrow

Research in cell biology applications beyond stem cell biology also often calls for sorting rare populations from complex mixtures for a variety of experimental endpoints. . If you’re involved in this work, you understand the critical need for a cell sorter that can both accurately identify these cells and isolate them with high purity. Here are three useful tips: 1. When sorting rare cells, not all will thrive when isolated in a plate. Consider exploring the use of conditioned media or feeder cells to support their survival and function. 2. Although faster sorting times are often preferred, they can lead to aborted events, resulting in insufficient material for downstream experiments. To reduce abort rates, consider sample enrichment before sorting or decreasing the event rate. 3. To minimize population spreading, which can occur with compensated multicolor samples, carefully select fluorescent dyes that minimize overlap. For more detailed guidance, refer to this application note. It outlines and demonstrates a method for isolating long-term hematopoietic stem cells (HSCs) from the bone marrow of adult mice. #cellbiology #stemcells #cellsorting #flowcytometry #cytoflexSRT #cellsorter #beckmancoulter #analis #analisdiagnostics