Anil van der Zee’s Post

📃Scientific paper: Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections Abstract: Objective Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli . Uropathogenic E. coli causes 80% of uncomplicated community acquired UTIs, particularly in pre-menopausal women. Considering this high prevalence and the potential to spread antimicrobial resistant genes, the current study was conducted to investigate the presence of clinically important strains of E. coli in Pakistani women having uncomplicated cystitis and pyelonephritis. Women belonging to low-income groups were exclusively included in the study. Seventy-four isolates from urine samples were processed, phylotyped, and screened for the presence of two Single Nucleotide Polymorphisms (SNPs) particularly associated with a clinically important clonal group A of E. coli (CgA) followed by antibiotic susceptibility testing and genome sequence analysis. Results Phylogroup B2 was most prevalent in patients and 44% of isolates were positive for the presence of CgA specific SNPs in Fumarate hydratase and DNA gyrase subunit B genes. Antibiotic susceptibility testing showed widespread resistance to trimethoprim-sulfamethoxazole and extended-spectrum beta-lactamase production. The infection analysis revealed the phylogroup B2 to be more pathogenic as compared to the other groups. The genome sequence of E. coli strain U17 revealed genes encoding virulence, multidrug resistance, and host colonization mechanisms. ... Continued on ES/IODE ➡️ https://etcse.fr/Qw9l ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

📃Scientific paper: Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections Abstract: Objective Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli . Uropathogenic E. coli causes 80% of uncomplicated community acquired UTIs, particularly in pre-menopausal women. Considering this high prevalence and the potential to spread antimicrobial resistant genes, the current study was conducted to investigate the presence of clinically important strains of E. coli in Pakistani women having uncomplicated cystitis and pyelonephritis. Women belonging to low-income groups were exclusively included in the study. Seventy-four isolates from urine samples were processed, phylotyped, and screened for the presence of two Single Nucleotide Polymorphisms (SNPs) particularly associated with a clinically important clonal group A of E. coli (CgA) followed by antibiotic susceptibility testing and genome sequence analysis. Results Phylogroup B2 was most prevalent in patients and 44% of isolates were positive for the presence of CgA specific SNPs in Fumarate hydratase and DNA gyrase subunit B genes. Antibiotic susceptibility testing showed widespread resistance to trimethoprim-sulfamethoxazole and extended-spectrum beta-lactamase production. The infection analysis revealed the phylogroup B2 to be more pathogenic as compared to the other groups. The genome sequence of E. coli strain U17 revealed genes encoding virulence, multidrug resistance, and host colonization mechanisms. ... Continued on ES/IODE ➡️ https://etcse.fr/Qw9l ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

San Diego University Researchers Believe Bacteriophages May Be the Future of Eradicating Multi-Drug Resistant Superbugs Clinical laboratories and microbiologists may soon have new powerful tools for fighting antimicrobial resistant bacteria that saves lives Superbugs—microbes that have developed multidrug resistance—continue to cause problems for clinical laboratories and hospital antibiotic stewardship programs around the world. Now, scientists at San Diego State University (SDSU) believe that bacteriophages (phages) could provide a solution for dealing with multi-drug resistant superbugs. Phages are miniscule, tripod-looking viruses that are genetically programmed to locate, attack, and eradicate a specific kind of pathogen. These microscopic creatures have saved lives and are being touted as a potential solution to superbugs, which are strains of bacteria, viruses, parasites, and fungi that are resistant to most antibiotics and other treatments utilized to counteract infections. “These multi-drug-resistant superbugs can cause chronic infections in individuals for months to years to sometimes decades,” Dwayne Roach, PhD, Assistant Professor of Bacteriophages, Infectious Disease, and Immunology at SDSU told CNN. “It’s ridiculous just how virulent some of these bacteria... Read the Dark Daily article at https://hubs.ly/Q02rHjxG0

San Diego University Researchers Believe Bacteriophages May Be the Future of Eradicating Multi-Drug Resistant Superbugs Clinical laboratories and microbiologists may soon have new powerful tools for fighting antimicrobial resistant bacteria that saves lives Superbugs—microbes that have developed multidrug resistance—continue to cause problems for clinical laboratories and hospital antibiotic stewardship programs around the world. Now, scientists at San Diego State University (SDSU) believe that bacteriophages (phages) could provide a solution for dealing with multi-drug resistant superbugs. Phages are miniscule, tripod-looking viruses that are genetically programmed to locate, attack, and eradicate a specific kind of pathogen. These microscopic creatures have saved lives and are being touted as a potential solution to superbugs, which are strains of bacteria, viruses, parasites, and fungi that are resistant to most antibiotics and other treatments utilized to counteract infections. “These multi-drug-resistant superbugs can cause chronic infections in individuals for months to years to sometimes decades,” Dwayne Roach, PhD, Assistant Professor of Bacteriophages, Infectious Disease, and Immunology at SDSU told CNN. “It’s ridiculous just how virulent some of these bacteria... Read the Dark Daily article at https://hubs.ly/Q02rHgSG0

Antibiotics can effectively target gut bacteria that harbor COVID-19 virus, study shows New research indicates that antibiotics can effectively target bacteria in the gut that harbor the virus that causes COVID-19 and produce toxin-like peptides that contribute to COVID-19-related symptoms. In the study, which involved 211 participants and was published in the Journal of Medical Virology, individuals who received early antibiotic treatment after having COVID-19 recovered more quickly than those who did not receive antibiotics. The authors had already evaluated the efficacy of certain antibiotics in SARS-CoV-2-infected bacterial cultures in vitro, and this new study demonstrates promising results with the use of the combination of 2 antibiotics (amoxicillin and rifaximin) within the first 3 days. Furthermore, a significant number of patients who received antibiotics within the first 3 days and for a duration of 7 days during the acute phase of COVID-19 did not develop long COVID. "Our findings suggest that antibiotics should be considered in acute infection and Long COVID. The study also lays the foundation for additional vaccine strategies." : Marina Piscopo, PhD, co-corresponding author of the University of Naples Federico, Italy Posted by Larry Cole

Creighton's groundbreaking research is making a difference. 🔬🤝 Sudhanva Kashyap, PhD, assistant professor in the Department of Microbiology and Immunology, has received a $410k grant from the National Institute of Health (NIH) and the National Institute of Allergy and Infectious Disease (NIAID) to combat human-parasitic infections. Long interested in the complexities of parasitic worms, Kashyap will explore the challenges posed by soil-transmitted helminth parasites, including the molecular mechanisms underlying parasitic recovery from drug-induced paralysis. His goal? To develop more effective treatment strategies and ultimately eliminate these neglected tropical diseases to improve the lives of more than 1 billion people worldwide. 🌎 Learn more ➡️ https://lnkd.in/ghRNtGYe

The Kleist group has an open PhD project in the IMPRS-BAC graduate school open call! The lab is interested in modelling the efficacy of pharmaceutical interventions in preventing viral infection (HIV-1), as well as in modelling the interplay between immunity and viral evolution (SARS-CoV-2). It is hypothesized that the evolution of SARS-CoV-2 is largely driven by population immunity and the ability to cross-neutralize incident variants. In order to study this phenomenon, the group combines deep mutational scanning data with (molecular) surveillance data and infection history to quantify, for each emerging strain, the strain-specific expected number of susceptibles. Quantifying the clinical efficacy of preventive therapies (vaccine, prophylaxis) poses an immense statistical (and monetary) challenge: As an example, a single COVID vaccine had to be tested on 40,000 individuals to obtain ~100 evaluable data points (= infections) distributed over two intervention arms (placebo, e.g., 95 infections vs. vaccine, 5 infections). To allow investigate dose-effect relationships and to derive a mechanistic understanding of the mode of action of preventive measures, the group develops integrative stochastic modelling and simulation techniques in the field of systems medicine and systems pharmacology. They use these models in collaboration with clinical- and epidemiological partners at RKI, John Hopkins, and Harvard to consult decision-makers and to develop guidelines with the WHO. 👓 More details on the project page: https://lnkd.in/eDZH3Z2j ✍️ Apply here: https://lnkd.in/dGUAXeNy

Glad to finally see some recognition on what we have been espousing for for well over a year now… that the Holy Grail of Long COVID lies within the gut microbiome. Our 75% response rate in our Long COVID population to our NuBalance Protocol (which involves an antibiotic pretreat followed with a thorough purge and a microbiome restoration step with OTC probiotics) is inexpensive, well tolerated and effective. Gastroenterologists are already performing over 12 million colonoscopies each year, and therefore should be the logical “experts” on microbiome restoration. Yet most Long COVID clinics rarely have GI input… Strange… Our mantra remains: Save your Gut and Save your World.

Despite clear evidence, chronic Lyme disease remains controversial. Don't miss our webinar on June 13th, where Dr. Embers, an Associate Professor in the Division of Immunology and the Director of Vector-borne Disease Research at the Tulane National Primate Research Center, will discuss why Lyme persists and how her recent research may help to address it. More about the research: Dr. Embers and a team of researchers from Tulane University have found that a combination of antibiotics is more effective in treating Lyme disease than the commonly prescribed course of one single antibiotic. Their results could pave the way for improved Lyme disease treatments, particularly in persistent cases that have not responded to standard treatment with a single antibiotic. Learn more or sign up now for this free event: https://lnkd.in/e2JMVSab #projectlyme #lyme #lymediseaseawareness #coinfections #tickbornediseases #chroniclyme #fightlyme #event #webinar

In a recent science review published in Trends in Microbiology, #BGI-Research highlights the potential of cell-free DNA (cfDNA) as a non-invasive biomarker for the diagnosis, treatment, and prognosis of infectious diseases from both pathogen and host perspectives, along with the challenges and possible solutions in clinical settings. Read: https://bit.ly/3YwuPS1