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KickStart is thrilled to celebrate Wubin Bai and his team for their participation in UNC’s first Research & Discovery Fair! The Bai Lab was one of…
KickStart is thrilled to celebrate Wubin Bai and his team for their participation in UNC’s first Research & Discovery Fair! The Bai Lab was one of…
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Poland Defers Implementation of GM Feed Ban https://lnkd.in/etwcinky
Poland Defers Implementation of GM Feed Ban https://lnkd.in/etwcinky
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MARA Issues New and Renewed Biosafety Certificates for GE Crops in China https://lnkd.in/eaZqWaEB
MARA Issues New and Renewed Biosafety Certificates for GE Crops in China https://lnkd.in/eaZqWaEB
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Experience
Education
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Madurai Kamaraj University
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Thesis title: Development of a transformation system for Vigna mungo and analysis of the DNA B clone (KA27) of Mungbean yellow mosaic virus-Vigna.
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Licenses & Certifications
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ETS Program and Audit Training Course
Excellence Through Stewardship
Issued
Publications
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Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation
Nature Communications
Agrobacterium-mediated plant transformation (AMT) is the basis of modern-day plant biotechnology. One major drawback of this technology is the recalcitrance of many plant species/varieties to Agrobacterium infection, most likely caused by elicitation of plant defense responses. Here, we develop a strategy to increase AMT by engineering Agrobacterium tumefaciens to express a type III secretion system (T3SS) from Pseudomonas syringae and individually deliver the P. syringae effectors AvrPto…
Agrobacterium-mediated plant transformation (AMT) is the basis of modern-day plant biotechnology. One major drawback of this technology is the recalcitrance of many plant species/varieties to Agrobacterium infection, most likely caused by elicitation of plant defense responses. Here, we develop a strategy to increase AMT by engineering Agrobacterium tumefaciens to express a type III secretion system (T3SS) from Pseudomonas syringae and individually deliver the P. syringae effectors AvrPto, AvrPtoB, or HopAO1 to suppress host defense responses. Using the engineered Agrobacterium, we demonstrate increase in AMT of wheat, alfalfa and switchgrass by ~250%–400%. We also show that engineered A. tumefaciens expressing a T3SS can deliver a plant protein, histone H2A-1, to enhance AMT. This strategy is of great significance to both basic research and agricultural biotechnology for transient and stable transformation of recalcitrant plant species/varieties and to deliver proteins into plant cells in a non-transgenic manner.
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Overexpression of VIRE2-INTERACTING PROTEIN2 in Arabidopsis regulates genes involved in Agrobacterium-mediated plant transformation and abiotic stresses
Nature Scientific Reports
Arabidopsis VIRE2-INTERACTING PROTEIN2 (VIP2) was previously described as a protein with a NOT domain, and Arabidopsis vip2 mutants are recalcitrant to Agrobacterium-mediated root transformation. Here we show that VIP2 is a transcription regulator and the C-terminal NOT2 domain of VIP2 interacts with VirE2. Interestingly, AtVIP2 overexpressor lines in Arabidopsis did not show an improvement in Agrobacterium-mediated stable root transformation, but the transcriptome analysis identified 1,634…
Arabidopsis VIRE2-INTERACTING PROTEIN2 (VIP2) was previously described as a protein with a NOT domain, and Arabidopsis vip2 mutants are recalcitrant to Agrobacterium-mediated root transformation. Here we show that VIP2 is a transcription regulator and the C-terminal NOT2 domain of VIP2 interacts with VirE2. Interestingly, AtVIP2 overexpressor lines in Arabidopsis did not show an improvement in Agrobacterium-mediated stable root transformation, but the transcriptome analysis identified 1,634 differentially expressed genes compared to wild-type. These differentially expressed genes belonged to various functional categories such as membrane proteins, circadian rhythm, signaling, response to stimulus, regulation of plant hypersensitive response, sequence-specific DNA binding transcription factor activity and transcription regulatory region binding. In addition to regulating genes involved in Agrobacterium-mediated plant transformation, AtVIP2 overexpressor line showed differential expression of genes involved in abiotic stresses. The majority of the genes involved in abscisic acid (ABA) response pathway, containing the Abscisic Acid Responsive Element (ABRE) element within their promoters, were down-regulated in AtVIP2 overexpressor lines. Consistent with this observation, AtVIP2 overexpressor lines were more susceptible to ABA and other abiotic stresses. Based on the above findings, we hypothesize that VIP2 not only plays a role in Agrobacterium-mediated plant transformation but also acts as a general transcriptional regulator in plants.
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Agrobacterium T-DNA integration into the plant genome can occur without the activity of key non-homologous end-joining proteins
The Plant Journal/Wiley Online Library in association with Society for Experimental Biology (SEB)
Balaji Vasudevan, So-Yon Park, Zarir E Vaghchhipawala contributed equally to this research work and the manuscript
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Transformation of Phytophthora capsici with genes for green and red fluorescent protein for use in visualizing plant-pathogen interactions
Australasian Plant Pathology/Springer Link
Performed molecular analysis of Phytophthora capsici harboring GFP and RFP genes in plasmids. Using Southern hybridization analysis, determined the copy number of GFP and RFP in multiple P. capsici transformants that were later used for in planta assays in pepper plants
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Agrobacterium may delay plant non-homologous end-joining DNA repair via XRCC4 to favor T-DNA integration
The Plant Cell/American Society of Plant Biology (ASPB)
The mechanism of Agrobacterium T-DNA integration is unclear. Non-homologous end-joining mediated dsDNA repair pathway was proposed as the major model for T-DNA integration. We here show that a key NHEJ protein XRCC4 limits T-DNA integration. Also evidence is presented for the first time that Agrobacterium may target XRCC4 by the VirE2 interaction to delay NHEJ-mediated dsDNA repair and favor T-DNA integration and genetic transformation.
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Over-expression of snakin-2 and extensin-like protein genes restricts pathogen invasiveness and enhances tolerance to Clavibacter michiganensis subsp. michiganensis in transgenic tomato (Solanum lycopersicum)
Transgenic Research/Springer Link
Showed for the first time that transgenic tomato expressing anti-microbial peptide Snakin-2 and cell wall extensin-like protein (ELP) were highly tolerant to Clavibacter michiganensis subsp.michiganensis (Cmm) infection and restricted pathogen growth and disease development. Demonstrated that SN2 and ELP over-expression limits Cmm invasiveness suggesting potential in vivo antibacterial activity and possible biotechnological application for these two defense proteins
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Silencing of Host Basal Defense Response-Related Gene Expression Increases Susceptibility of Nicotiana benthamiana to Clavibacter michiganensis subsp. michiganensis
Phytopathology/American Phytopathological Society (APS)
This paper was chosen as Editor-in-Chief top pick for March 2011 issue of the journal Phytopathology (http://www.apsnet.org/publications/Emails/APSUpdate38.htm#LETTER.BLOCK9)
For the first time, Nicotiana benthamiana was developed as a model and alternative host plant for Clavibacter michiganensis subsp. michiganensis (Cmm), a Gram-positive bacterium that caused wilt and canker disease on tomato. Showed Cmm can infect and cause typical symptoms in N. benthamiana as in the host plant…This paper was chosen as Editor-in-Chief top pick for March 2011 issue of the journal Phytopathology (http://www.apsnet.org/publications/Emails/APSUpdate38.htm#LETTER.BLOCK9)
For the first time, Nicotiana benthamiana was developed as a model and alternative host plant for Clavibacter michiganensis subsp. michiganensis (Cmm), a Gram-positive bacterium that caused wilt and canker disease on tomato. Showed Cmm can infect and cause typical symptoms in N. benthamiana as in the host plant tomato. These results paved the way for functional genomics studies using VIGS as forward and reverse genetics to dissect the molecular plant-Cmm interaction and explore for plant genes that can confer tolerance/resistance to Cmm disease.
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Resistance to crown gall disease in transgenic grapevine rootstocks containing truncated virE2 of Agrobacterium
Transgenic Research/Springer Link
Showed truncated form of Agrobacterium protein VirE2 when expressed in transgenic grapevine can confer tolerance/resistance to crown gall disease caused by Agrobacterium vitis.
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Activation and manipulation of host responses by a Gram-positive bacterium
Plant Signaling and Behaviour/Society of Plant Signaling and Behavior and Taylor & Francis Online
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Tomato transcriptional changes in response to Clavibacter michiganensis subsp. michiganensis reveal a role for ethylene in disease development
Plant Physiology/American Society of Plant Biology (ASPB)
First study to show tomato transcriptome changes in response to Clavibacter michiganensis subsp. michiganensis (Cmm) infection and disease development
For the very first tine, microarray data using tomato Affymetrix chip was generated and deposited in Tomato Functional Genomics Database (TFGD) at Cornell University
(http://ted.bti.cornell.edu/cgi-bin/TFGD/miame/experiment.cgi?ID=E024)Other authors -
Transcriptional analysis of the tomato resistance response triggered by recognition of the Xanthomonas type III effector AvrXv3
Functional & Integrative Genomics/Springer Link
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Identification and expression profiling of tomato genes differentially regulated during a resistance response to Xanthomonas campestris pv. Vesicatoria
Molecular Plant-Microbe Interactions/American Phytopathological Society (APS)
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Infectivity analysis of two variable DNA B components of Mungbean yellow mosaic virus-Vigna (MYMV-Vig) in Vigna mungo and Vigna radiata
Journal of Biosciences/Springer Link
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Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B component
Archives of Virology/Springer Link
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Agrobacterium-mediated Transformation Efficiency in Blackgram and Rice Enhanced by Multiple Copies of pTiBo542 virB and virG
Indian Journal of Biotechnology (IJBT)/NISCAIR-CSIR, India
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Efficient vir gene induction in Agrobacterium tumefaciens requires virA, virG and vir box from the same Ti plasmid
Journal of Bacteriology/American Society for Microbiology (ASM)
Languages
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English
Full professional proficiency
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Tamil
Full professional proficiency
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Telugu
Elementary proficiency
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Bolivia Approves Planting of GM Soybean Intacta https://lnkd.in/ecGj4m9T
Bolivia Approves Planting of GM Soybean Intacta https://lnkd.in/ecGj4m9T
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USDA APHIS Releases Regulatory Status Review Responses to CoverCress, GCMBNA, Moolec, and MSU Pennycress with lower erucic acid, fiber, and…
USDA APHIS Releases Regulatory Status Review Responses to CoverCress, GCMBNA, Moolec, and MSU Pennycress with lower erucic acid, fiber, and…
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HIRING!!! #hiring #jobposting Need someone with technical project management experience to run some of our projects. Comfortable being client…
HIRING!!! #hiring #jobposting Need someone with technical project management experience to run some of our projects. Comfortable being client…
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"Academic entrepreneur" might strike some as an oxymoron, but the interest among university faculty to launch startups has grown dramatically over…
"Academic entrepreneur" might strike some as an oxymoron, but the interest among university faculty to launch startups has grown dramatically over…
Liked by Balaji Vasudevan
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Super cool to see NC IDEA and NCInnovation teaming up to work with our NC public universities and the commercialization of the great ideas which…
Super cool to see NC IDEA and NCInnovation teaming up to work with our NC public universities and the commercialization of the great ideas which…
Liked by Balaji Vasudevan
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Excited to announce that I will be attending the World Agtech Summit in Mexico! Looking forward to connecting with innovative startups, investors…
Excited to announce that I will be attending the World Agtech Summit in Mexico! Looking forward to connecting with innovative startups, investors…
Liked by Balaji Vasudevan
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Congratulations to our portfolio company EnFuego Therapeutics founded by University of North Carolina at Chapel Hill's Chad Pecot! We are very happy…
Congratulations to our portfolio company EnFuego Therapeutics founded by University of North Carolina at Chapel Hill's Chad Pecot! We are very happy…
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