- Voiced the Cadbury's Caramel Bunny in the British adverts.
- Since 1968, her partner has been Australian academic Heather Sutherland. Became an Australian citizen on 26 January 2013. She was part of the Australia Day ceremony attended by Prime Minister Julia Gillard (who was born in Wales).
- Playing Madame Morrible in the London production of Wicked. (December 2006)
- Lived and worked in the U.S. for 16 years.
- She is a huge admirer of the works of Charles Dickens and has toured the world in a one-woman show, Dickens' Women, inspired by the females in his works.
- She was awarded O.B.E. (Officer of the Order of the British Empire) in the 2002 Queen's New Years Honours List for her services to drama.
- Canberra, Australia: Becoming a naturalised Australian citizen - "I've wanted to be an Australian for a very long time. My partner is Australian." (January 2013)
- Despite playing their grandmother in The Age of Innocence (1993), Margoyles is just 17 years older than Michelle Pfeiffer and 30 years older than Winona Ryder.
- Aged 21 or 22 she was a contestant on ITV quiz show 'University Challenge' representing Newnham College, Cambridge in 1963. Although the videotape no longer exists, still off-air photographs of her on the team panel do survive and can be found online. Apparently she swore after answering a question wrong and this had to be edited out of the broadcast tape.
- Of Belarussian-Jewish descent, she is the daughter of Joseph (1899-1995) and Ruth (née Walters) Margolyes (1905-1974). She took time out of her career to nurse her mother and spent a quarter of a million pounds on full-time care for her father. She supports charities for disabled people and their careers.
- Appearing in the Broadway production of "Wicked". (April 2008)
- Attended Oxford High School GDST.
- Lifelong friends with Jane Cussons, Patricia Hodge and Mary Tamm, her co-stars in The Girls of Slender Means (1975).
- Was offered the role of Professor Laird in Resurrection of the Daleks: Part One (1984). Chole Ashcroft took the role.
- Despite playing her mother in The Age of Innocence (1993), Margoyles is just 3 years older than Geraldine Chaplin.
- In the Independent on Sunday [UK] 2006 Pink List - a list of the most influential gay men and women - Margolyes came no. 93, down from last year's no. 29.
- When asked in an interview which of her own film was her personal favorite, she named Sunshine (1999) starring Ralph Fiennes.
- Is a massive Arsenal FC supporter.
- She underwent heart valve replacement surgery in 2023.
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