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Accelerating Pharmaceutical Advancements Through Partnership
HORIBA's solutions for pharmaceutical analysis and manufacturing
What is Protein Analysis
Techniques like infrared (IR), Fluorescence, and Raman spectroscopy can analyze the vibrations of protein molecules, providing information about their secondary structure, such as alpha helices, beta sheets, and turns. Spectroscopic techniques can monitor changes in protein structure upon binding to ligands or undergoing post-translational modifications, providing information on protein function and regulation.
Aggregation of proteins is another critical quality attribute (CQA) that can significantly affect the efficacy of therapeutics, techniques like Dynamic Light Scattering (DLS), Laser Diffraction, and NanoTracking Analysis (NTA) can shed light on the size distribution range and in the case of NTA, the absolute concentration (number of particles) between integral limits. This has utility in many up and coming technologies like exosomes, and has shown to be crucial to established techniques like liposomal vaccines.
Protein analysis is the cornerstone of modern biological research, employing sophisticated techniques to unveil the complex architecture, dynamic functions, and critical interactions of proteins. As the workhorses of cellular machinery, proteins orchestrate essential biological processes and serve as primary targets for therapeutic intervention.
These intricate biomolecules, built from precise sequences of amino acids, hold the key to understanding disease mechanisms, drug responses, and cellular regulation. Through rigorous analytical methods, researchers can:
HORIBA's state-of-the-art technology portfolio enables:
Through our advanced analytical platforms, HORIBA empowers researchers worldwide to accelerate scientific discoveries, enhance therapeutic development, and advance our understanding of life's fundamental processes. Our dedication to analytical excellence drives breakthrough research across biochemistry, biotechnology, medicine, and genetics.
Spectroscopic methods offer powerful tools for analyzing proteins, each bringing unique advantages to research and quality control. UV-Visible spectroscopy provides fast and simple protein quantification with non-destructive analysis, minimal sample preparation, and real-time measurement capabilities, making it a cost-effective choice for many applications.
Fluorescence spectroscopy
Fluorescence spectroscopy delivers extremely sensitive detection and excels in protein folding studies, measuring protein-protein interactions, and providing dynamic information, all while requiring minimal sample volumes.
Raman spectroscopy
Raman spectroscopy stands out for its label-free protein analysis capabilities, performing excellently in aqueous solutions while providing detailed structural information without destroying samples. The technique requires minimal sample preparation and minimal sample volumes, making it highly practical for routine analysis.
Particle Characterization
Monitoring protein aggregation and particle formation is crucial, as proteins can degrade under various stresses including temperature fluctuations, shear forces, high concentrations, and extended storage time. By measuring particle concentration and size distribution, researchers can effectively compare different samples and treatment conditions to optimize protein stability.
At HORIBA, we recognize the importance of these analytical techniques and offer advanced instrumentation across several platforms. Our portfolio specifically excels in high-sensitivity fluorescence spectroscopy, state-of-the-art Raman systems, and precise UV-Visible analysis, providing researchers with the tools they need for comprehensive protein characterization.
荧光及吸收光谱仪
高灵敏一体式荧光光谱仪
模块化科研级稳瞬态荧光光谱
Fluorescence Lifetime Imaging Platform
全自动模块化荧光寿命光谱仪
全自动模块化荧光寿命光谱仪
一种简单、快速、“无栏”的分子指纹技术
MicroRaman Spectrometer - Confocal Raman Microscope
Simultaneous Multi-Laser Nanoparticle Tracking Analysis (NTA)
台式拉曼光谱仪
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