PPP2CA

Proteinska fosfataza 2, katalitička podjedinica, alfa izoforma
Proteinska fosfataza 2, katalitička podjedinica, alfa izoforma
	PDB rendering based on 2iae.
Dostupne strukture
	2IAE, 2IE3, 2IE4, 2NPP, 2NYL, 2NYM, 3C5W, 3DW8, 3FGA, 3K7V, 3K7W, 3P71
Identifikatori
Simboli	PPP2CA; PP2Ac; PP2CA; PP2Calpha; RP-C
Vanjski ID	OMIM: 176915 MGI: 1321159 HomoloGene: 37660 GeneCards: PPP2CA Gene
EC broj	3.1.3.16
Ontologija gena
Molekularna funkcija	• aktivnost fosfoproteinskte fosfataze; • proteinsko vezivanje; • vezivanje proteinskog C-terminusa; • vezivanje metalnog jona; • aktivnost proteinske dimerizacije;
Celularna komponenta	• proteinska fosfataza tip 2A kompleks; • hromozom, centromerni region; • pol vretena; • nukleus; • mitohondrija;
Biološki proces	• nuklearno-transkribovani iRNK katabolički proces; • inaktivacija MAPK aktivnosti; • regulacija DNK replikacija; • regulacija transkripcije, DNK-zavisna;
Pregled RNK izražavanja
	podaci
Ortolozi
Vrsta	Čovek
Entrez	5515
Ensembl	ENSG00000113575
UniProt	P67775
RefSeq (mRNA)	NM_002715.2
RefSeq (protein)	NP_002706.1
Lokacija (UCSC)	Chr 5:; 133.53 - 133.56 Mb
PubMed pretraga	[1]

Serin/treonin proteinska fosfataza 2A katalitička podjedinica alfa izoforma je enzim koji je kod ljudi kodiran PPP2CA genom.^[1]

Proteinska fosfataza 2A je jedna od četiri glavne Ser/Thr fosfataze. Ona učestvuje u negativnoj kontroli ćelijskog rasta i deobe. Ona se sastoji od heteromerne enzimske osnove, koji ima katalitičku i konstantnu regulatornu podjedinicu, za koji se vezuje niz regulatornih podjedinica. Ovaj gen kodira alfa izoformu katalitičke podjedinice.^[2]

Interakcije

PPP2CA formira interakcije sa CCNG2,^[3] CTTNBP2,^[4] MOBKL3,^[4] Bestrofin 1,^[5] TLX1,^[6] Bcl-2,^[7] PPP2R3B,^[4]^[8] Ciklin-zavisna kinaza 2,^[9] STRN3,^[4] Ciklin-zavisna kinaza 6,^[9] IGBP1,^[4]^[10]^[11]^[12] PPP2R5E,^[4]^[13] STRN,^[4] PPP2R5D,^[4]^[13] PPP2R2A,^[4]^[14] PPP2R1B,^[4]^[15] PPP2R1A,^[4]^[15] CTTNBP2NL,^[4] FAM40A,^[4] PPP2R5C,^[4]^[13]^[16] PPP2R5B^[4]^[13] and PPP2R5A.^[4]^[13]

Vidi još

PPP2CB

Reference

^ Jones TA, Barker HM, da Cruz e Silva EF, Mayer-Jaekel RE, Hemmings BA, Spurr NK, Sheer D, Cohen PT (1993). „Localization of the genes encoding the catalytic subunits of protein phosphatase 2A to human chromosome bands 5q23→q31 and 8p12→p11.2, respectively”. Cytogenet Cell Genet. 63 (1): 35—41. PMID 8383590. doi:10.1159/000133497.
^ „Entrez Gene: PPP2CA protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform”.
^ Bennin, David A; Don Aruni S Arachchige; et al. (2002). „Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest”. J. Biol. Chem. United States. 277 (30): 27449—67. ISSN 0021-9258. PMID 11956189. doi:10.1074/jbc.M111693200.
^ ^а ^б ^в ^г ^д ^ђ ^е ^ж ^з ^и ^ј ^к ^л ^љ ^м ^н Goudreault, Marilyn; D'Ambrosio Lisa M; et al. (2009). „A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein”. Mol. Cell Proteomics. United States. 8 (1): 157—71. PMC 2621004 . PMID 18782753. doi:10.1074/mcp.M800266-MCP200.
^ Marmorstein, Lihua Y; McLaughlin Precious J; et al. (2002). „Bestrophin interacts physically and functionally with protein phosphatase 2A”. J. Biol. Chem. United States. 277 (34): 30591—7. ISSN 0021-9258. PMID 12058047. doi:10.1074/jbc.M204269200.
^ Kawabe, T; Muslin A J; et al. (1997). „HOX11 interacts with protein phosphatases PP2A and PP1 and disrupts a G2/M cell-cycle checkpoint”. Nature. ENGLAND. 385 (6615): 454—8. ISSN 0028-0836. PMID 9009195. doi:10.1038/385454a0.
^ Deng, X; Ito T; et al. (1998). „Reversible phosphorylation of Bcl2 following interleukin 3 or bryostatin 1 is mediated by direct interaction with protein phosphatase 2A”. J. Biol. Chem. UNITED STATES. 273 (51): 34157—63. ISSN 0021-9258. PMID 9852076. doi:10.1074/jbc.273.51.34157.
^ Yan, Z; Fedorov S A; et al. (2000). „PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells”. Mol. Cell. Biol. UNITED STATES. 20 (3): 1021—9. ISSN 0270-7306. PMC 85219 . PMID 10629059. doi:10.1128/MCB.20.3.1021-1029.2000.
^ ^а ^б Cheng, A; Kaldis P; Solomon M J (2000). „Dephosphorylation of human cyclin-dependent kinases by protein phosphatase type 2C alpha and beta 2 isoforms”. J. Biol. Chem. UNITED STATES. 275 (44): 34744—9. ISSN 0021-9258. PMID 10934208. doi:10.1074/jbc.M006210200.
^ Gingras, Anne-Claude; Caballero Michael; et al. (2005). „A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity”. Mol. Cell Proteomics. United States. 4 (11): 1725—40. ISSN 1535-9476. PMID 16085932. doi:10.1074/mcp.M500231-MCP200.
^ Chen, J; Peterson R T; et al. (1998). „Alpha 4 associates with protein phosphatases 2A, 4, and 6”. Biochem. Biophys. Res. Commun. UNITED STATES. 247 (3): 827—32. ISSN 0006-291X. PMID 9647778. doi:10.1006/bbrc.1998.8792.
^ Chung, H; Nairn A C; et al. (1999). „Mutation of Tyr307 and Leu309 in the protein phosphatase 2A catalytic subunit favors association with the alpha 4 subunit which promotes dephosphorylation of elongation factor-2”. Biochemistry. UNITED STATES. 38 (32): 10371—6. ISSN 0006-2960. PMID 10441131. doi:10.1021/bi990902g.
^ ^а ^б ^в ^г ^д McCright, B; Rivers A M; et al. (1996). „The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm”. J. Biol. Chem. UNITED STATES. 271 (36): 22081—9. ISSN 0021-9258. PMID 8703017. doi:10.1074/jbc.271.36.22081.
^ Kamibayashi, C; Lickteig R L; et al. (1992). „Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits”. J. Biol. Chem. UNITED STATES. 267 (30): 21864—72. ISSN 0021-9258. PMID 1328247.
^ ^а ^б Zhou, Jin; Pham Huong T; et al. (2003). „Characterization of the Aalpha and Abeta subunit isoforms of protein phosphatase 2A: differences in expression, subunit interaction, and evolution”. Biochem. J. England. 369 (Pt 2): 387—98. ISSN 0264-6021. PMC 1223084 . PMID 12370081. doi:10.1042/BJ20021244.
^ Ito, A; Kataoka T R; et al. (2000). „A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation”. EMBO J. ENGLAND. 19 (4): 562—71. ISSN 0261-4189. PMC 305863 . PMID 10675325. doi:10.1093/emboj/19.4.562.

Literatura

Cohen P, Cohen PT (1990). „Protein phosphatases come of age.”. J. Biol. Chem. 264 (36): 21435—8. PMID 2557326.
Zolnierowicz S (2000). „Type 2A protein phosphatase, the complex regulator of numerous signaling pathways.”. Biochem. Pharmacol. 60 (8): 1225—35. PMID 11007961. doi:10.1016/S0006-2952(00)00424-X.
Kamibayashi C; Lickteig RL; Estes R; et al. (1992). „Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits.”. J. Biol. Chem. 267 (30): 21864—72. PMID 1328247.
Goedert M, Cohen ES, Jakes R, Cohen P (1992). „p42 MAP kinase phosphorylation sites in microtubule-associated protein tau are dephosphorylated by protein phosphatase 2A1. Implications for Alzheimer's disease [corrected]”. FEBS Lett. 312 (1): 95—9. PMID 1330687. doi:10.1016/0014-5793(92)81418-L.
Khew-Goodall Y; Mayer RE; Maurer F; et al. (1991). „Structure and transcriptional regulation of protein phosphatase 2A catalytic subunit genes.”. Biochemistry. 30 (1): 89—97. PMID 1846293. doi:10.1021/bi00215a014.
Scheidtmann KH, Virshup DM, Kelly TJ (1991). „Protein phosphatase 2A dephosphorylates simian virus 40 large T antigen specifically at residues involved in regulation of DNA-binding activity.”. J. Virol. 65 (4): 2098—101. PMC 240073 . PMID 1848320.
Scheidtmann KH, Mumby MC, Rundell K, Walter G (1991). „Dephosphorylation of simian virus 40 large-T antigen and p53 protein by protein phosphatase 2A: inhibition by small-t antigen.”. Mol. Cell. Biol. 11 (4): 1996—2003. PMC 359885 . PMID 1848668.
Virshup DM, Kauffman MG, Kelly TJ (1990). „Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A.”. EMBO J. 8 (12): 3891—8. PMC 402079 . PMID 2555176.
Arino J; Woon CW; Brautigan DL; et al. (1988). „Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes.”. Proc. Natl. Acad. Sci. U.S.A. 85 (12): 4252—6. PMC 280405 . PMID 2837763. doi:10.1073/pnas.85.12.4252.
Stone SR; Mayer R; Wernet W; et al. (1989). „The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A alpha catalytic subunit.”. Nucleic Acids Res. 16 (23): 11365. PMC 339016 . PMID 2849764. doi:10.1093/nar/16.23.11365.
Carrey EA, Campbell DG, Hardie DG (1986). „Phosphorylation and activation of hamster carbamyl phosphate synthetase II by cAMP-dependent protein kinase. A novel mechanism for regulation of pyrimidine nucleotide biosynthesis.”. EMBO J. 4 (13B): 3735—42. PMC 554725 . PMID 4092695.
McCright B, Virshup DM (1995). „Identification of a new family of protein phosphatase 2A regulatory subunits.”. J. Biol. Chem. 270 (44): 26123—8. PMID 7592815. doi:10.1074/jbc.270.44.26123.
Favre B, Zolnierowicz S, Turowski P, Hemmings BA (1994). „The catalytic subunit of protein phosphatase 2A is carboxyl-methylated in vivo.”. J. Biol. Chem. 269 (23): 16311—7. PMID 8206937.
Redpath NT, Price NT, Severinov KV, Proud CG (1993). „Regulation of elongation factor-2 by multisite phosphorylation.”. Eur. J. Biochem. 213 (2): 689—99. PMID 8386634. doi:10.1111/j.1432-1033.1993.tb17809.x.
Muramatsu T, Kincaid RL (1993). „Molecular cloning of a full-length cDNA encoding the catalytic subunit of human calmodulin-dependent protein phosphatase (calcineurin A alpha).”. Biochim. Biophys. Acta. 1178 (1): 117—20. PMID 8392375. doi:10.1016/0167-4889(93)90117-8.
Kissinger CR; Parge HE; Knighton DR; et al. (1996). „Crystal structures of human calcineurin and the human FKBP12-FK506-calcineurin complex.”. Nature. 378 (6557): 641—4. PMID 8524402. doi:10.1038/378641a0.
Ohguro H; Rudnicka-Nawrot M; Buczyłko J; et al. (1996). „Structural and enzymatic aspects of rhodopsin phosphorylation.”. J. Biol. Chem. 271 (9): 5215—24. PMID 8617805. doi:10.1074/jbc.271.9.5215.
McCright B, Rivers AM, Audlin S, Virshup DM (1996). „The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm.”. J. Biol. Chem. 271 (36): 22081—9. PMID 8703017. doi:10.1074/jbc.271.36.22081.

[pmid8383590-1] Jones TA, Barker HM, da Cruz e Silva EF, Mayer-Jaekel RE, Hemmings BA, Spurr NK, Sheer D, Cohen PT (1993). „Localization of the genes encoding the catalytic subunits of protein phosphatase 2A to human chromosome bands 5q23→q31 and 8p12→p11.2, respectively”. Cytogenet Cell Genet. 63 (1): 35—41. PMID 8383590. doi:10.1159/000133497.

[2] „Entrez Gene: PPP2CA protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform”.

[pmid11956189-3] Bennin, David A; Don Aruni S Arachchige; et al. (2002). „Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest”. J. Biol. Chem. United States. 277 (30): 27449—67. ISSN 0021-9258. PMID 11956189. doi:10.1074/jbc.M111693200.

[pmid18782753-4] а ^б ^в ^г ^д ^ђ ^е ^ж ^з ^и ^ј ^к ^л ^љ ^м ^н Goudreault, Marilyn; D'Ambrosio Lisa M; et al. (2009). „A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein”. Mol. Cell Proteomics. United States. 8 (1): 157—71. PMC 2621004 . PMID 18782753. doi:10.1074/mcp.M800266-MCP200.

[pmid12058047-5] Marmorstein, Lihua Y; McLaughlin Precious J; et al. (2002). „Bestrophin interacts physically and functionally with protein phosphatase 2A”. J. Biol. Chem. United States. 277 (34): 30591—7. ISSN 0021-9258. PMID 12058047. doi:10.1074/jbc.M204269200.

[pmid9009195-6] Kawabe, T; Muslin A J; et al. (1997). „HOX11 interacts with protein phosphatases PP2A and PP1 and disrupts a G2/M cell-cycle checkpoint”. Nature. ENGLAND. 385 (6615): 454—8. ISSN 0028-0836. PMID 9009195. doi:10.1038/385454a0.

[pmid9852076-7] Deng, X; Ito T; et al. (1998). „Reversible phosphorylation of Bcl2 following interleukin 3 or bryostatin 1 is mediated by direct interaction with protein phosphatase 2A”. J. Biol. Chem. UNITED STATES. 273 (51): 34157—63. ISSN 0021-9258. PMID 9852076. doi:10.1074/jbc.273.51.34157.

[pmid10629059-8] Yan, Z; Fedorov S A; et al. (2000). „PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells”. Mol. Cell. Biol. UNITED STATES. 20 (3): 1021—9. ISSN 0270-7306. PMC 85219 . PMID 10629059. doi:10.1128/MCB.20.3.1021-1029.2000.

[pmid10934208-9] а ^б Cheng, A; Kaldis P; Solomon M J (2000). „Dephosphorylation of human cyclin-dependent kinases by protein phosphatase type 2C alpha and beta 2 isoforms”. J. Biol. Chem. UNITED STATES. 275 (44): 34744—9. ISSN 0021-9258. PMID 10934208. doi:10.1074/jbc.M006210200.

[pmid16085932-10] Gingras, Anne-Claude; Caballero Michael; et al. (2005). „A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity”. Mol. Cell Proteomics. United States. 4 (11): 1725—40. ISSN 1535-9476. PMID 16085932. doi:10.1074/mcp.M500231-MCP200.

[pmid9647778-11] Chen, J; Peterson R T; et al. (1998). „Alpha 4 associates with protein phosphatases 2A, 4, and 6”. Biochem. Biophys. Res. Commun. UNITED STATES. 247 (3): 827—32. ISSN 0006-291X. PMID 9647778. doi:10.1006/bbrc.1998.8792.

[pmid10441131-12] Chung, H; Nairn A C; et al. (1999). „Mutation of Tyr307 and Leu309 in the protein phosphatase 2A catalytic subunit favors association with the alpha 4 subunit which promotes dephosphorylation of elongation factor-2”. Biochemistry. UNITED STATES. 38 (32): 10371—6. ISSN 0006-2960. PMID 10441131. doi:10.1021/bi990902g.

[pmid8703017-13] а ^б ^в ^г ^д McCright, B; Rivers A M; et al. (1996). „The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm”. J. Biol. Chem. UNITED STATES. 271 (36): 22081—9. ISSN 0021-9258. PMID 8703017. doi:10.1074/jbc.271.36.22081.

[pmid1328247-14] Kamibayashi, C; Lickteig R L; et al. (1992). „Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits”. J. Biol. Chem. UNITED STATES. 267 (30): 21864—72. ISSN 0021-9258. PMID 1328247.

[pmid12370081-15] а ^б Zhou, Jin; Pham Huong T; et al. (2003). „Characterization of the Aalpha and Abeta subunit isoforms of protein phosphatase 2A: differences in expression, subunit interaction, and evolution”. Biochem. J. England. 369 (Pt 2): 387—98. ISSN 0264-6021. PMC 1223084 . PMID 12370081. doi:10.1042/BJ20021244.

[pmid10675325-16] Ito, A; Kataoka T R; et al. (2000). „A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation”. EMBO J. ENGLAND. 19 (4): 562—71. ISSN 0261-4189. PMC 305863 . PMID 10675325. doi:10.1093/emboj/19.4.562.

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