Gelanska lijaza
| Gelanska lijaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.2.25 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Gelanska lijaza (EC 4.2.2.25) je enzim sa sistematskim imenom gelan beta-D-glukopiranozil-(1->4)-D-glukopiranoziluronat lijaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Eliminativno razlaganje beta-D-glukopiranozil-(1->4)-beta-D-glukopiranoziluronatnih veza gelanske osnove uz odvajanje tetrasaharida koji sadrži 4-dezoksi-4,5-nezasićenu D-glukopiranoziluronsku kiselinu na neredukujućem kraju. Tetrasaharid formiran iz deacetilisanog gelana je beta-D-4-dezoksi-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alfa-L-Rhap-(1->3)-beta-D-Glcp.
Ovaj enzim je veoma specifičan za gelan, i posebno za deacetilisani gelan.
- ↑ Hashimoto, W., Maesaka, K., Sato, N., Kimura, S., Yamamoto, K., Kumagai, H. and Murata, K. (1997). „Microbial system for polysaccharide depolymerization: enzymatic route for gellan depolymerization by Bacillus sp. GL1”. Arch. Biochem. Biophys. 339: 17-23. PMID 9056228.
- ↑ Hashimoto, W., Sato, N., Kimura, S. and Murata, K. (1998). „Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1”. Arch. Biochem. Biophys. 354: 31-39. PMID 9633595.
- ↑ Miyake, O., Kobayashi, E., Nankai, H., Hashimoto, W., Mikami, B. and Murata, K. (2004). „Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp. GL1”. Arch. Biochem. Biophys. 422: 211-220. PMID 14759609.
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