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[ 源代码: trimmomatic  ]

软件包:trimmomatic(0.39 dfsg-2)

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flexible read trimming tool for Illumina NGS data

Trimmomatic performs a variety of useful trimming tasks for illumina paired-end and single ended data.The selection of trimming steps and their associated parameters are supplied on the command line.

The current trimming steps are:

 * ILLUMINACLIP: Cut adapter and other illumina-specific sequences from
   the read.
 * SLIDINGWINDOW: Perform a sliding window trimming, cutting once thes
   average quality within the window falls below a threshold.
 * LEADING: Cut bases off the start of a read, if below a threshold quality
 * TRAILING: Cut bases off the end of a read, if below a threshold quality
 * CROP: Cut the read to a specified length
 * HEADCROP: Cut the specified number of bases from the start of the read
 * MINLENGTH: Drop the read if it is below a specified length
 * TOPHRED33: Convert quality scores to Phred-33
 * TOPHRED64: Convert quality scores to Phred-64
It works with FASTQ (using phred 33 or phred 64 quality scores, depending on the Illumina pipeline used), either uncompressed or gzipp'ed FASTQ. Use of gzip format is determined based on the .gz extension.

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