Standard BioTools

The development and progression of #prostatecancer is a multi-step process that can be difficult to predict. High-plex imaging is supporting current research working to expand the limited information about the immune infiltrate and cancer cell behavior in this disease. This image shows β-catenin (red), which can correspond with a high risk of death due to tumor progression. Low CD8 (green) density can also be a poor prognostic marker and included in a risk score for biochemical and metastatic relapse. Vimentin (blue) is a marker for epithelial-mesenchymal transition, with potential to develop therapeutics targeting vimentin along with other surface markers to prevent cancer metastases or kill cancer stem cells. Granzyme B (yellow) is expressed by cytotoxic T lymphocytes and natural killer cells to induce apoptosis in tumors and can be used as an indicator of T cell activation in tumors. Get more details on this and other applications for high-plex tissue imaging: https://lnkd.in/g8aHFdSF #spatialproteomics #immunology #tissueimaging #translationalresearch 

Why choose a service project for cytometry or imaging studies? To take advantage of the latest technologies, leveraging experts who can help your studies move quickly. Explore the Standard BioTools™ Discovery Labs comprehensive menu of standard and custom services aligned to your project goals, budget and timelines. Our global labs are available for instrument demonstrations, service projects and much more. Take a look and get a tour: https://lnkd.in/gywMZubZ #DiscoveryLabs #worldwide #labservices

The “one pathogen, one disease” theory is shifting to complex multiple pathogen disease cases. One example of this is in aquaculture, where recirculating aquaculture systems are used to reduce water consumption and wastewater generation, but growing production is leading to increased risk of introducing pathogens at farming sites. New research from DTU - Technical University of Denmark and published in Scientific Reports provides potential for early detection and prevention with a method of detecting multiple pathogens and putative pathogens responsible for co-infections in aquaculture. The method uses high-throughput qPCR on the Standard BioTools™ 48.48 dynamic array to screen for a large number of pathogens simultaneously, allowing rapid repeated sampling and enabling an understanding of trends and fluctuations in relative pathogen loads that could be predictive of increased health risks or disease outbreaks. Sørensen et al. 2024: https://lnkd.in/gbaR-BUQ #fisheries #pathogendetection #virology #immunology

Using targeted sets of markers combined into panels offers a jump start into high-plex imaging. Let’s look at an example… Three specialized tissue imaging panels support neurodegenerative spatial biology studies. When combined with larger panels tailored for neurophenotyping, tissue architecture, and immune cell profiling, three different high-parameter 41-marker imaging panels for neurodegenerative diseases can be easily assembled. Use the application note linked here to learn how these panels work together and the types of protein markers they cover, identifying amyloid aggregates and Tau tangles, Lewy bodies and the loss of major myelin components: https://lnkd.in/giECekUW #neurodegeneration #neuroscience #spatialbiology #alzheimersdisease #parkinsonsdisease #multiplesclerosis

Cardiovascular disease, defined as a range of conditions that affect the heart and blood vessels, is the leading cause of death globally, taking an estimated 17.9 million lives each year. Truly understanding cardiovascular disease, and therefore mitigating its impact, requires deep exploration of a complex cellular landscape.  Take a look through a collection of publications that highlight how technology can help change how we study cardiological health threats: https://lnkd.in/g_mSq3vT #cardiovascularresearch #hearthealth

Congratulations to Jonathan Sussman, Ben Stanger and team on their new publication in Cancer Research and their cover image for the issue! Their new study provides insights into the spatial heterogeneity of pancreatic ductal adenocarcinoma, in which a hypovascular and hypoxic environment can alter tumor cell behavior and limit therapeutic efficacy. Imaging Mass Cytometry™ revealed that pancreatic ductal cancers are composed of 10 distinct cellular neighborhoods, including a vascular niche enriched for macrophages expressing high levels of CD44 and a proangiogenic gene signature. Sussman et al. 2024: https://lnkd.in/gHeM-GGA #pancreaticcancer #cancerresearch #multiplexedimaging

Today is #WorldBrainDay, promoting awareness and education for neurological disorders that impact global health through conditions such as stroke, migraine, Alzheimer's, meningitis, and epilepsy. Many of these conditions can be prevented, warranting in-depth analysis of preventative measures. Technologies such as Imaging Mass Cytometry™ that can image the brain at a cellular, and even subcellular, level are supporting neurological research into pathological proteins that are thought to be a common mechanism for the progression of various neurodegenerative diseases. A cellular understanding of healthy states and disease states can inform on early changes in the brain and the potential for effective therapeutic approaches. More on the day: https://lnkd.in/dQiTq4FU More on neuroscience with IMC™: https://lnkd.in/giECekUW #neuroscience #highpleximaging #brainhealth

The spatial location of markers in a breast cancer sample can offer a wealth of information on protein expression, cell activation, metastasis, disease progression and more. Here, a multiplexed whole slide image by Imaging Mass Cytometry™ highlights how markers can indicate breast cancer progression and prognosis in cancer research. Elevated fibroblast activation protein-α (FAP) in yellow can be associated with negative clinical outcomes across most cancer types, with a role in promoting tumor growth, invasion, metastasis, and immunosuppression. Collagen I (dark blue) has been shown to augment the aggressive characteristics of breast cancer cells, and immune cell markers CD45 (teal) and CD68 (pink) demonstrate the number of immune cells in tumor tissue and indicate tumor grade and other malignant features, respectively. Get more details on this and other applications for high-plex tissue imaging: https://lnkd.in/g8aHFdSF #spatialproteomics #immunology #tissueimaging #translationalresearch 

Here’s an interesting weekend read… on a new approach to understanding the interpatient heterogeneity of malignant cells. Using #CYTOF profiling of cells from cancer patients to ID #RNAseq cell types, researchers at Amsterdam UMC were able to accurately unveil hidden RNA profiles within the tumor microenvironment. The team developed a malignant cell fraction-informed RNA deconvolution method, OncoBLADE, and evaluated the method experimentally using bulk RNA profiles with 19 CyTOF determined cell fractions, and in silico using 180,177 single-cell RNA profiles. Kim et al. 2024: https://lnkd.in/gXSdDbbi #braincancer #lungcancer #cellheterogeneity

An advantage of working with metal tagged antibodies for multiplexed tissue imaging is that they facilitate faster panel design (no autofluorescence or overlap to manage). And predesigned panels make this even easier, because several panels can fit together or be rearranged to capture targets of interest for a given study. For example, an Immuno-Oncology IMC Panel can be combined with an Immune Cell Expansion Panel to characterize the tumor immune landscape. Or a Tissue Architecture IMC Panel, Stromal Cell Panel and Basic Immune Panel can be used to detect the underlying structural composition of normal and tumorous tissue and the presence of stromal cell and immune cell subtypes… and then add in some additional antibodies if desired. See how these panels can work together in our #applicationnote on multiplexed whole slide imaging of the tumor microenvironment: https://lnkd.in/g8rA6GvP #immunooncology #spatialbiology #spatialanalysis #focusonthescience