Attività
-
It’s been a blast to spend three days at the #EUROoCS2024 conference! I had the pleasure to share my work entitled “On-chip tracking of human…
It’s been a blast to spend three days at the #EUROoCS2024 conference! I had the pleasure to share my work entitled “On-chip tracking of human…
Consigliato da Paola Occhetta
-
Call for 2025 World Congress Session Proposals! The OARSI Program Committee is reaching out to the OA research community for program themes towards…
Call for 2025 World Congress Session Proposals! The OARSI Program Committee is reaching out to the OA research community for program themes towards…
Consigliato da Paola Occhetta
-
The annual EUROoCs conference #EUROoCS2024 is a “must” for European researchers working on microphysiological systems. This years event in Milano was…
The annual EUROoCs conference #EUROoCS2024 is a “must” for European researchers working on microphysiological systems. This years event in Milano was…
Consigliato da Paola Occhetta
Esperienza
-
BiomimX Srl
-
-
-
-
-
-
-
-
-
-
-
-
Formazione
Pubblicazioni
-
Fabrication of 3D cell-laden hydrogel microstructures through photo-mold patterning
Biofabrication 2013
Native tissues are characterized by spatially organized three-dimensional (3D) microscaled units which functionally define cells–cells and cells–extracellular matrix interactions. The ability to engineer biomimetic constructs mimicking these 3D microarchitectures is subject to the control over cell distribution and organization. In the present study we introduce a novel protocol to generate 3D cell laden hydrogel micropatterns with defined size and shape. The method, named photo-mold patterning…
Native tissues are characterized by spatially organized three-dimensional (3D) microscaled units which functionally define cells–cells and cells–extracellular matrix interactions. The ability to engineer biomimetic constructs mimicking these 3D microarchitectures is subject to the control over cell distribution and organization. In the present study we introduce a novel protocol to generate 3D cell laden hydrogel micropatterns with defined size and shape. The method, named photo-mold patterning (PMP), combines hydrogel micromolding within polydimethylsiloxane (PDMS) stamps and photopolymerization through a recently introduced biocompatible ultraviolet (UVA) activated photoinitiator (VA-086). Exploiting PDMS micromolds as geometrical constraints for two methacrylated prepolymers (polyethylene glycol diacrylate and gelatin methacrylate), micrometrically resolved structures were obtained within a 3 min exposure to a low cost and commercially available UVA LED. The PMP was validated both on a continuous cell line (human umbilical vein endothelial cells expressing green fluorescent protein, HUVEC GFP) and on primary human bone marrow stromal cells (BMSCs). HUVEC GFP and BMSCs were exposed to 1.5% w/v VA-086 and UVA light (1 W, 385 nm, distance from sample = 5 cm). Photocrosslinking conditions applied during the PMP did not negatively affect cells viability or specific metabolic activity. Quantitative analyses demonstrated the potentiality of PMP to uniformly embed viable cells within 3D microgels, creating biocompatible and favorable environments for cell proliferation and spreading during a seven days' culture. PMP can thus be considered as a promising and cost effective tool for designing spatially accurate in vitro models and, in perspective, functional constructs.
Altri autoriVedi pubblicazione
Lingue
-
Italiano
Conoscenza madrelingua o bilingue
-
Inglese
-
Altre attività di Paola
-
Great EUROOC 2024 conference In Milano last week! Thank you to the organizers, in particular Paola Occhetta and Marco Rasponi the two chairs! It was…
Great EUROOC 2024 conference In Milano last week! Thank you to the organizers, in particular Paola Occhetta and Marco Rasponi the two chairs! It was…
Consigliato da Paola Occhetta
-
The EUROoCS conference 2024 in Milano just came to an end 🇮🇹🍕🍹. It was great to finally see each other again and to meet a lot of new people I…
The EUROoCS conference 2024 in Milano just came to an end 🇮🇹🍕🍹. It was great to finally see each other again and to meet a lot of new people I…
Consigliato da Paola Occhetta
-
The #EUROoCS Conference 2024 has come to an end. I am delighted to have been immersed in cutting-edge science regarding organ-on-chip and…
The #EUROoCS Conference 2024 has come to an end. I am delighted to have been immersed in cutting-edge science regarding organ-on-chip and…
Consigliato da Paola Occhetta
-
Going back to Italy is always amazing for me, but going back to an event like this, full of people with A LOT to say, give and teach, is simply…
Going back to Italy is always amazing for me, but going back to an event like this, full of people with A LOT to say, give and teach, is simply…
Consigliato da Paola Occhetta
-
The PRIN2022 #VISION project made its debut at its first conferenc: #EUROoCS2024. Great job, Giovanni Buccioli!
The PRIN2022 #VISION project made its debut at its first conferenc: #EUROoCS2024. Great job, Giovanni Buccioli!
Consigliato da Paola Occhetta
-
🎯 EUROoCS 2024 - what an organization & event! 👍Marco Rasponi & Paola Occhetta set a wonderful venue at Politecnico di Milano. 👏Brilliant keynote…
🎯 EUROoCS 2024 - what an organization & event! 👍Marco Rasponi & Paola Occhetta set a wonderful venue at Politecnico di Milano. 👏Brilliant keynote…
Consigliato da Paola Occhetta
-
Another wonderful EUROoCS meeting came to an end! We had over 600 people joined us in Milan, with many old and new friends we spent 3 days discussing…
Another wonderful EUROoCS meeting came to an end! We had over 600 people joined us in Milan, with many old and new friends we spent 3 days discussing…
Consigliato da Paola Occhetta
-
Heading back home from a fantastic EUROoCS 2024 meeting in #Milano #Italy with a lot of inspiration. 👨🏫 At the meeting Laurent Barbe presented…
Heading back home from a fantastic EUROoCS 2024 meeting in #Milano #Italy with a lot of inspiration. 👨🏫 At the meeting Laurent Barbe presented…
Consigliato da Paola Occhetta
-
The EUROoCS2024 conference is officially over. A huge thank you to everyone who attended! Your participation made it a truly inspiring event. Special…
The EUROoCS2024 conference is officially over. A huge thank you to everyone who attended! Your participation made it a truly inspiring event. Special…
Consigliato da Paola Occhetta
-
On the last day of the EUROoCS 2024 conference in Milan, our #NOCI researchers had the opportunity to share their results. Specifically, Alireza…
On the last day of the EUROoCS 2024 conference in Milan, our #NOCI researchers had the opportunity to share their results. Specifically, Alireza…
Consigliato da Paola Occhetta
-
Milano, it’s a #wrap ! This years EUROoCS was full of intense discussions, awesome science and excellent food! 😌🍷🍕Happy to have pitched MPSlabs…
Milano, it’s a #wrap ! This years EUROoCS was full of intense discussions, awesome science and excellent food! 😌🍷🍕Happy to have pitched MPSlabs…
Consigliato da Paola Occhetta
-
📢 Last day at EUROoCS Annual Meeting 2024! 🤩 EURL ECVAM EU Science, Research and Innovation is excited to participate in several activities linked…
📢 Last day at EUROoCS Annual Meeting 2024! 🤩 EURL ECVAM EU Science, Research and Innovation is excited to participate in several activities linked…
Consigliato da Paola Occhetta
-
Really enjoyed the EUROoCS Conference at Politecnico di Milano! It was a pleasure to attend with the amazing team from imec. Had a fantastic time…
Really enjoyed the EUROoCS Conference at Politecnico di Milano! It was a pleasure to attend with the amazing team from imec. Had a fantastic time…
Consigliato da Paola Occhetta
-
Buongiorno Milano! ☀️👋🏻 🚀 After this epic social dinner last night at the National Museum of Science & Technology Leonardo da Vinci, our team is…
Buongiorno Milano! ☀️👋🏻 🚀 After this epic social dinner last night at the National Museum of Science & Technology Leonardo da Vinci, our team is…
Consigliato da Paola Occhetta
Altri profili simili
Altre persone che si chiamano Paola Occhetta
Su LinkedIn ci sono altre 2 persone che si chiamano Paola Occhetta
Vedi altre persone che si chiamano Paola Occhetta