Attività
-
🚀 We’ve just kicked off the 2024 #OrganOnChip Summer School in Tübingen! 🚀 Just as last year, we’ve teamed up with the EUROoCS’s “Teaching &…
🚀 We’ve just kicked off the 2024 #OrganOnChip Summer School in Tübingen! 🚀 Just as last year, we’ve teamed up with the EUROoCS’s “Teaching &…
Consigliato da Paola Occhetta
-
My beautiful fiancé Roberta Tihomirova “was looking for a husband” 👀 and I am proud she found me. I didn’t automatically get scared of the…
My beautiful fiancé Roberta Tihomirova “was looking for a husband” 👀 and I am proud she found me. I didn’t automatically get scared of the…
Consigliato da Paola Occhetta
Esperienza
-
BiomimX Srl
-
-
-
-
-
-
-
-
-
-
-
-
Formazione
Pubblicazioni
-
Fabrication of 3D cell-laden hydrogel microstructures through photo-mold patterning
Biofabrication 2013
Native tissues are characterized by spatially organized three-dimensional (3D) microscaled units which functionally define cells–cells and cells–extracellular matrix interactions. The ability to engineer biomimetic constructs mimicking these 3D microarchitectures is subject to the control over cell distribution and organization. In the present study we introduce a novel protocol to generate 3D cell laden hydrogel micropatterns with defined size and shape. The method, named photo-mold patterning…
Native tissues are characterized by spatially organized three-dimensional (3D) microscaled units which functionally define cells–cells and cells–extracellular matrix interactions. The ability to engineer biomimetic constructs mimicking these 3D microarchitectures is subject to the control over cell distribution and organization. In the present study we introduce a novel protocol to generate 3D cell laden hydrogel micropatterns with defined size and shape. The method, named photo-mold patterning (PMP), combines hydrogel micromolding within polydimethylsiloxane (PDMS) stamps and photopolymerization through a recently introduced biocompatible ultraviolet (UVA) activated photoinitiator (VA-086). Exploiting PDMS micromolds as geometrical constraints for two methacrylated prepolymers (polyethylene glycol diacrylate and gelatin methacrylate), micrometrically resolved structures were obtained within a 3 min exposure to a low cost and commercially available UVA LED. The PMP was validated both on a continuous cell line (human umbilical vein endothelial cells expressing green fluorescent protein, HUVEC GFP) and on primary human bone marrow stromal cells (BMSCs). HUVEC GFP and BMSCs were exposed to 1.5% w/v VA-086 and UVA light (1 W, 385 nm, distance from sample = 5 cm). Photocrosslinking conditions applied during the PMP did not negatively affect cells viability or specific metabolic activity. Quantitative analyses demonstrated the potentiality of PMP to uniformly embed viable cells within 3D microgels, creating biocompatible and favorable environments for cell proliferation and spreading during a seven days' culture. PMP can thus be considered as a promising and cost effective tool for designing spatially accurate in vitro models and, in perspective, functional constructs.
Altri autoriVedi pubblicazione
Lingue
-
Italiano
Conoscenza madrelingua o bilingue
-
Inglese
-
Altre attività di Paola
-
#Hiring Project Associate position available at our lab @ National Centre for Biological Sciences. Check out details
#Hiring Project Associate position available at our lab @ National Centre for Biological Sciences. Check out details
Consigliato da Paola Occhetta
-
Here we go again! 🚀 BrainOMIC Season 2 is approaching! Join us at Human Technopole in Milan for an exciting week of training in computational…
Here we go again! 🚀 BrainOMIC Season 2 is approaching! Join us at Human Technopole in Milan for an exciting week of training in computational…
Consigliato da Paola Occhetta
-
Doctors have begun trialling the world’s first #mRNA #lungcancer #vaccine in patients, as experts hailed its “groundbreaking” potential to save…
Doctors have begun trialling the world’s first #mRNA #lungcancer #vaccine in patients, as experts hailed its “groundbreaking” potential to save…
Consigliato da Paola Occhetta
-
The Safety Pharmacology Society Annual Meeting is less than a month away!! Are you joining?? ✏ Registrations are still open:…
The Safety Pharmacology Society Annual Meeting is less than a month away!! Are you joining?? ✏ Registrations are still open:…
Consigliato da Paola Occhetta
-
Enjoy your holiday. Do it for your loved ones. 🌴 In Europe, it's vacation season. People are taking a well-deserved break. But let's think beyond…
Enjoy your holiday. Do it for your loved ones. 🌴 In Europe, it's vacation season. People are taking a well-deserved break. But let's think beyond…
Consigliato da Paola Occhetta
-
Happy to announce that OncoBone has received Tempo funding from Business Finland for piloting our Bone Metastasis Technology Platform.
Happy to announce that OncoBone has received Tempo funding from Business Finland for piloting our Bone Metastasis Technology Platform.
Consigliato da Paola Occhetta
-
A topic close to my heart, written with my friends and collaborators from social sciences.
A topic close to my heart, written with my friends and collaborators from social sciences.
Consigliato da Paola Occhetta
-
🫀Decoding Cardiac Fibrosis: New Study Sheds Light on ECM's Role in Heart Disease In recent papers published in Translational Research, researchers…
🫀Decoding Cardiac Fibrosis: New Study Sheds Light on ECM's Role in Heart Disease In recent papers published in Translational Research, researchers…
Consigliato da Paola Occhetta
Altri profili simili
Altre persone che si chiamano Paola Occhetta
Su LinkedIn ci sono altre 2 persone che si chiamano Paola Occhetta
Vedi altre persone che si chiamano Paola Occhetta