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Ratiometric Imaging plugin for uManager

This is Java application, designed for CCD/CMOS camera-based fluorescence measurements.

Features

  • Designed with ratiometric imaging tasks in mind.
  • Automatic management of experimental data/labnotes storage. All naming, saving, comment templating is done automatically.
  • Online and offline analysis of fluorescence in ROIs with automatic background correction.
  • Works on Windows, expected (but not tested) to work on MacOS.

Coming soon:

  • Automated experimental flow. Describe your drug application order and timing once. Then, put your sample, press Start and wait. (Requires remotely controlled valves)

Requirements

  • Works within uManager 2.0 framework (https://micro-manager.org/) as a plugin (based on API 2.0)
  • Requires either LPT port or other digital state device (e.g. NI card with digital outs) to control Sutter filter wheel and camera shutter.

Instructions to compile and run

  1. JDK 1.6 (for uManager plugins) and Netbeans 8 /JDK1.7 installed. This is a Netbeans project, and is expected to compile without problems.
  2. If you don’t have experience in writing plugins for uManager, please read this and this before trying to compile the project.
  3. In order to use the plugin, place RMI2.jar to \Micro-Manager-2.0beta\mmplugins
  4. Create \Micro-Manager-2.0beta\mmplugins\lib folder and place org-jdesktop-layout.jar and swing-layout-1.0.4.jar to it.

Please, also see the Wiki for some details

Alternatively, simply download precompiled RMI.jar (along with putting org-jdesktop-layout.jar and swing-layout-1.0.4.jar to \mmplugins\lib folder) and run uManager

Enjoy and contribute!

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MicroManager 2.0 plugin for ratiometric widefield imaging

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