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This is a package for working with imaging cytometry data.

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ifcimglib

Install

Run:

pip install .

to install this package.

How to use

cif2lmdb can be used as follows:

Usage: cif2lmdb [OPTIONS] CIF

Options:
  --output FILE       Output filename. If not set, cif-filename is taken with
                      lmdb extension.

  --channels INTEGER  Images from these channels will be extracted. Default is
                      to extract all. 1-based index.

  --names TEXT        Names to assign to channels.
  --debug             Show debugging information. Limits output to 100 first
                      cells.

  --overwrite         Overwrite lmdb if it exists.
  --targets-npy FILE  Numpy binary file containing targets.
  --skip-npy FILE     Numpy binary file containing instances to be skipped.
  --help              Show this message and exit.

Here is an example command:

cif2lmdb --channels 1 --channels 6 --channels 9 --names BF --names SSC --names BF2 --output tmp.lmdb --debug --overwrite input.cif

It takes input.cif as input and outputs output.lmdb, an lmdb-file containing 100 (see debug flag) 3-channel images with names BF, SSC and BF2.

Please see the imglmdb.ipynb notebook for usage examples of the imglmdb package.

Docker

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Docker images are available for cif2lmdb (tag cif2lmdb), the jupyter lab environment (tag jupyter-lab-env), and the notebooks in this repository (tag notebooks).

For using cif2lmdb, run:

docker run --rm -v /path/to/data/dir:/data maximlippeveld/ifcimglib:cif2lmdb [OPTIONS] /data/example.cif

For using the jupyter environment, run:

docker run --it -v /path/to/data/dir:/data -v /path/to/your/code/dir:/app -p [your-port]:8888 maximlippeveld/ifcimglib:jupyter-lab-env

Fur using the notebooks in this repository, run:

docker run --it -v /path/to/data/dir:/data -p [your-port]:8888 maximlippeveld/ifcimglib:notebooks

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This is a package for working with imaging cytometry data.

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