Vasodilator-stimulated phosphoprotein

VASP
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1EGX, 1USD, 1USE, 2PAV, 2PBD, 3CHW
Identifiers
Aliases	VASP, vasodilator-stimulated phosphoprotein, vasodilator stimulated phosphoprotein
External IDs	OMIM: 601703; MGI: 109268; HomoloGene: 7592; GeneCards: VASP; OMA:VASP - orthologs
Gene location (Human) Chr. Chromosome 19 (human) Band 19q13.32 Start 45,506,579 bp End 45,526,983 bp
Gene location (Mouse) Chr. Chromosome 7 (mouse) Band 7|7 A3 Start 18,990,854 bp End 19,005,742 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in granulocyte monocyte mucosa of transverse colon saphenous vein spleen blood muscle layer of sigmoid colon rectum thoracic aorta ascending aorta Top expressed in granulocyte mesenteric lymph nodes tibiofemoral joint colon spleen left colon left lung lobe pyloric antrum epithelium of stomach atrium More reference expression data BioGPS More reference expression data
Gene ontology Molecular function profilin binding SH3 domain binding protein binding actin binding cadherin binding Cellular component cell projection membrane focal adhesion bicellular tight junction filopodium plasma membrane cell junction actin cytoskeleton extracellular exosome cytoskeleton lamellipodium membrane lamellipodium filopodium membrane cytoplasm cytosol Biological process positive regulation of actin filament polymerization axon guidance neural tube closure actin polymerization or depolymerization protein homotetramerization actin cytoskeleton organization cell junction assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7408 22323 Ensembl ENSG00000125753 ENSMUSG00000030403 UniProt P50552 P70460 RefSeq (mRNA) NM_001008736 NM_003370 NM_001282021 NM_001282022 NM_009499 RefSeq (protein) NP_003361 NP_001268950 NP_001268951 NP_033525 Location (UCSC) Chr 19: 45.51 – 45.53 Mb Chr 7: 18.99 – 19.01 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Vasodilator-stimulated phosphoprotein is a protein that in humans is encoded by the VASP gene.

Function

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an N-terminal EVH1 domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions cell membranes. In the mid-region of the protein, family members have a proline-rich region that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Interactions

Vasodilator-stimulated phosphoprotein has been shown to interact with Zyxin, Profilin 1, and PFN2.

Further reading

• Reinhard M, Halbrügge M, Scheer U, et al. (1992). "The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts". EMBO J. 11 (6): 2063–70. doi:10.1002/j.1460-2075.1992.tb05264.x. PMC 556672. PMID 1318192.2063-70&rft.date=1992&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC556672#id-name=PMC&rft_id=info:pmid/1318192&rft_id=info:doi/10.1002/j.1460-2075.1992.tb05264.x&rft.aulast=Reinhard&rft.aufirst=M&rft.au=Halbrügge, M&rft.au=Scheer, U&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC556672&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Halbrügge M, Eigenthaler M, Polke C, Walter U (1992). "Protein phosphorylation regulated by cyclic nucleotide-dependent protein kinases in cell extracts and in intact human lymphocytes". Cell. Signal. 4 (2): 189–99. doi:10.1016/0898-6568(92)90082-J. PMID 1319722.189-99&rft.date=1992&rft_id=info:doi/10.1016/0898-6568(92)90082-J&rft_id=info:pmid/1319722&rft.aulast=Halbrügge&rft.aufirst=M&rft.au=Eigenthaler, M&rft.au=Polke, C&rft.au=Walter, U&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Reinhard M, Jouvenal K, Tripier D, Walter U (1995). "Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein)". Proc. Natl. Acad. Sci. U.S.A. 92 (17): 7956–60. Bibcode:1995PNAS...92.7956R. doi:10.1073/pnas.92.17.7956. PMC 41265. PMID 7644520.7956-60&rft.date=1995&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC41265#id-name=PMC&rft_id=info:pmid/7644520&rft_id=info:doi/10.1073/pnas.92.17.7956&rft_id=info:bibcode/1995PNAS...92.7956R&rft.aulast=Reinhard&rft.aufirst=M&rft.au=Jouvenal, K&rft.au=Tripier, D&rft.au=Walter, U&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC41265&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Reinhard M, Giehl K, Abel K, et al. (1995). "The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins". EMBO J. 14 (8): 1583–9. doi:10.1002/j.1460-2075.1995.tb07146.x. PMC 398250. PMID 7737110.1583-9&rft.date=1995&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC398250#id-name=PMC&rft_id=info:pmid/7737110&rft_id=info:doi/10.1002/j.1460-2075.1995.tb07146.x&rft.aulast=Reinhard&rft.aufirst=M&rft.au=Giehl, K&rft.au=Abel, K&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC398250&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Haffner C, Jarchau T, Reinhard M, et al. (1995). "Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP". EMBO J. 14 (1): 19–27. doi:10.1002/j.1460-2075.1995.tb06971.x. PMC 398048. PMID 7828592.19-27&rft.date=1995&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC398048#id-name=PMC&rft_id=info:pmid/7828592&rft_id=info:doi/10.1002/j.1460-2075.1995.tb06971.x&rft.aulast=Haffner&rft.aufirst=C&rft.au=Jarchau, T&rft.au=Reinhard, M&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC398048&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Horstrup K, Jablonka B, Hönig-Liedl P, et al. (1994). "Phosphorylation of focal adhesion vasodilator-stimulated phosphoprotein at Ser157 in intact human platelets correlates with fibrinogen receptor inhibition". Eur. J. Biochem. 225 (1): 21–7. doi:10.1111/j.1432-1033.1994.00021.x. PMID 7925440.21-7&rft.date=1994&rft_id=info:doi/10.1111/j.1432-1033.1994.00021.x&rft_id=info:pmid/7925440&rft.aulast=Horstrup&rft.aufirst=K&rft.au=Jablonka, B&rft.au=Hönig-Liedl, P&rft_id=https://doi.org/10.1111%2Fj.1432-1033.1994.00021.x&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Butt E, Abel K, Krieger M, et al. (1994). "cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets". J. Biol. Chem. 269 (20): 14509–17. doi:10.1016/S0021-9258(17)36652-8. PMID 8182057.14509-17&rft.date=1994&rft_id=info:doi/10.1016/S0021-9258(17)36652-8&rft_id=info:pmid/8182057&rft.aulast=Butt&rft.aufirst=E&rft.au=Abel, K&rft.au=Krieger, M&rft_id=https://doi.org/10.1016%2FS0021-9258%2817%2936652-8&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Laurent V, Loisel TP, Harbeck B, et al. (1999). "Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes". J. Cell Biol. 144 (6): 1245–58. doi:10.1083/jcb.144.6.1245. PMC 2150578. PMID 10087267.1245-58&rft.date=1999&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150578#id-name=PMC&rft_id=info:pmid/10087267&rft_id=info:doi/10.1083/jcb.144.6.1245&rft.aulast=Laurent&rft.aufirst=V&rft.au=Loisel, TP&rft.au=Harbeck, B&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2150578&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Bachmann C, Fischer L, Walter U, Reinhard M (1999). "The EVH2 domain of the vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding, and actin bundle formation". J. Biol. Chem. 274 (33): 23549–57. doi:10.1074/jbc.274.33.23549. PMID 10438535.23549-57&rft.date=1999&rft_id=info:doi/10.1074/jbc.274.33.23549&rft_id=info:pmid/10438535&rft.aulast=Bachmann&rft.aufirst=C&rft.au=Fischer, L&rft.au=Walter, U&rft.au=Reinhard, M&rft_id=https://doi.org/10.1074%2Fjbc.274.33.23549&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Petit MM, Fradelizi J, Golsteyn RM, et al. (2000). "LPP, an actin cytoskeleton protein related to zyxin, harbors a nuclear export signal and transcriptional activation capacity". Mol. Biol. Cell. 11 (1): 117–29. doi:10.1091/mbc.11.1.117. PMC 14761. PMID 10637295.117-29&rft.date=2000&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC14761#id-name=PMC&rft_id=info:pmid/10637295&rft_id=info:doi/10.1091/mbc.11.1.117&rft.aulast=Petit&rft.aufirst=MM&rft.au=Fradelizi, J&rft.au=Golsteyn, RM&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC14761&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Krause M, Sechi AS, Konradt M, et al. (2000). "Fyn-binding protein (Fyb)/SLP-76-associated protein (SLAP), Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton". J. Cell Biol. 149 (1): 181–94. doi:10.1083/jcb.149.1.181. PMC 2175102. PMID 10747096.181-94&rft.date=2000&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175102#id-name=PMC&rft_id=info:pmid/10747096&rft_id=info:doi/10.1083/jcb.149.1.181&rft.aulast=Krause&rft.aufirst=M&rft.au=Sechi, AS&rft.au=Konradt, M&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175102&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Drees B, Friederich E, Fradelizi J, et al. (2000). "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins". J. Biol. Chem. 275 (29): 22503–11. doi:10.1074/jbc.M001698200. PMID 10801818.22503-11&rft.date=2000&rft_id=info:doi/10.1074/jbc.M001698200&rft_id=info:pmid/10801818&rft.aulast=Drees&rft.aufirst=B&rft.au=Friederich, E&rft.au=Fradelizi, J&rft_id=https://doi.org/10.1074%2Fjbc.M001698200&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Smolenski A, Poller W, Walter U, Lohmann SM (2000). "Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I". J. Biol. Chem. 275 (33): 25723–32. doi:10.1074/jbc.M909632199. PMID 10851246.25723-32&rft.date=2000&rft_id=info:doi/10.1074/jbc.M909632199&rft_id=info:pmid/10851246&rft.aulast=Smolenski&rft.aufirst=A&rft.au=Poller, W&rft.au=Walter, U&rft.au=Lohmann, SM&rft_id=https://doi.org/10.1074%2Fjbc.M909632199&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Harbeck B, Hüttelmaier S, Schluter K, et al. (2000). "Phosphorylation of the vasodilator-stimulated phosphoprotein regulates its interaction with actin". J. Biol. Chem. 275 (40): 30817–25. doi:10.1074/jbc.M005066200. PMID 10882740.30817-25&rft.date=2000&rft_id=info:doi/10.1074/jbc.M005066200&rft_id=info:pmid/10882740&rft.aulast=Harbeck&rft.aufirst=B&rft.au=Hüttelmaier, S&rft.au=Schluter, K&rft_id=https://doi.org/10.1074%2Fjbc.M005066200&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Burkhardt M, Glazova M, Gambaryan S, et al. (2000). "KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells". J. Biol. Chem. 275 (43): 33536–41. doi:10.1074/jbc.M005670200. PMID 10922374.33536-41&rft.date=2000&rft_id=info:doi/10.1074/jbc.M005670200&rft_id=info:pmid/10922374&rft.aulast=Burkhardt&rft.aufirst=M&rft.au=Glazova, M&rft.au=Gambaryan, S&rft_id=https://doi.org/10.1074%2Fjbc.M005670200&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Ball LJ, Kühne R, Hoffmann B, et al. (2000). "Dual epitope recognition by the VASP EVH1 domain modulates polyproline ligand specificity and binding affinity". EMBO J. 19 (18): 4903–14. doi:10.1093/emboj/19.18.4903. PMC 314220. PMID 10990454.4903-14&rft.date=2000&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC314220#id-name=PMC&rft_id=info:pmid/10990454&rft_id=info:doi/10.1093/emboj/19.18.4903&rft.aulast=Ball&rft.aufirst=LJ&rft.au=Kühne, R&rft.au=Hoffmann, B&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC314220&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Bearer EL, Prakash JM, Manchester RD, Allen PG (2001). "VASP protects actin filaments from gelsolin: an in vitro study with implications for platelet actin reorganizations". Cell Motil. Cytoskeleton. 47 (4): 351–64. doi:10.1002/1097-0169(200012)47:4<351::AID-CM8>3.0.CO;2-8. PMC 3376085. PMID 11093254.351-64&rft.date=2001&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3376085#id-name=PMC&rft_id=info:pmid/11093254&rft_id=info:doi/10.1002/1097-0169(200012)47:4<351::AID-CM8>3.0.CO;2-8&rft.aulast=Bearer&rft.aufirst=EL&rft.au=Prakash, JM&rft.au=Manchester, RD&rft.au=Allen, PG&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3376085&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Lawrence DW, Pryzwansky KB (2001). "The vasodilator-stimulated phosphoprotein is regulated by cyclic GMP-dependent protein kinase during neutrophil spreading". J. Immunol. 166 (9): 5550–6. doi:10.4049/jimmunol.166.9.5550. PMID 11313394.5550-6&rft.date=2001&rft_id=info:doi/10.4049/jimmunol.166.9.5550&rft_id=info:pmid/11313394&rft.aulast=Lawrence&rft.aufirst=DW&rft.au=Pryzwansky, KB&rft_id=https://doi.org/10.4049%2Fjimmunol.166.9.5550&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">
• Castellano F, Le Clainche C, Patin D, et al. (2001). "A WASp-VASP complex regulates actin polymerization at the plasma membrane". EMBO J. 20 (20): 5603–14. doi:10.1093/emboj/20.20.5603. PMC 125672. PMID 11598004.5603-14&rft.date=2001&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC125672#id-name=PMC&rft_id=info:pmid/11598004&rft_id=info:doi/10.1093/emboj/20.20.5603&rft.aulast=Castellano&rft.aufirst=F&rft.au=Le Clainche, C&rft.au=Patin, D&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC125672&rfr_id=info:sid/en.wikipedia.org:Vasodilator-stimulated phosphoprotein" class="Z3988">