NFYC

Nuclear transcription factor Y subunit gamma is a protein that in humans is encoded by the NFYC gene.

NFYC
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1N1J, 4AWL, 4CSR
Identifiers
Aliases	NFYC, CBF-C, CBFC, H1TF2A, HAP5, HSM, NF-YC, nuclear transcription factor Y subunit gamma
External IDs	OMIM: 605344; MGI: 107901; HomoloGene: 7440; GeneCards: NFYC; OMA:NFYC - orthologs
Gene location (Human) Chr. Chromosome 1 (human) Band 1p34.2 Start 40,691,648 bp End 40,771,603 bp
Gene location (Mouse) Chr. Chromosome 4 (mouse) Band 4 D2.1|4 56.52 cM Start 120,614,635 bp End 120,688,769 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in buccal mucosa cell apex of heart muscle of thigh monocyte tendon of biceps brachii right lobe of thyroid gland right auricle gastrocnemius muscle ganglionic eminence skin of abdomen Top expressed in secondary oocyte zygote primary oocyte genital tubercle tail of embryo thymus heart cerebellar cortex bone marrow olfactory bulb More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA-binding transcription factor activity RNA polymerase II cis-regulatory region sequence-specific DNA binding DNA binding transcription coactivator activity DNA-binding transcription activator activity, RNA polymerase II-specific protein binding protein heterodimerization activity DNA-binding transcription factor activity, RNA polymerase II-specific transcription factor binding sequence-specific DNA binding Cellular component CCAAT-binding factor complex protein-DNA complex nucleus nucleoplasm RNA polymerase II transcription regulator complex Biological process regulation of transcription by RNA polymerase II positive regulation of transcription, DNA-templated regulation of transcription, DNA-templated transcription by RNA polymerase II protein folding transcription, DNA-templated positive regulation of transcription by RNA polymerase II regulation of cholesterol biosynthetic process negative regulation of transcription, DNA-templated Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4802 18046 Ensembl ENSG00000066136 ENSMUSG00000032897 UniProt Q13952 P70353 RefSeq (mRNA) NM_001142587 NM_001142588 NM_001142589 NM_001142590 NM_001308114 NM_001308115 NM_014223 NM_001048168 NM_001277095 NM_008692 NM_001357192 NM_001357193 NM_001357194 RefSeq (protein) NP_001136059 NP_001136060 NP_001136061 NP_001136062 NP_001295043 NP_001295044 NP_055038 NP_001041633 NP_001264024 NP_032718 NP_001344121 NP_001344122 NP_001344123 Location (UCSC) Chr 1: 40.69 – 40.77 Mb Chr 4: 120.61 – 120.69 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit C, forms a tight dimer with the B subunit (NFYB), a prerequisite for subunit A (NFYA) association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.

Two microRNAs; miR-30c and miR-30e are located within introns of the nfyc gene. These microRNAs are actively transcribed in human insulin-producing beta cells in the pancreatic islets that also show high expression of nfyc and CDH1 genes. The expression of these intronic microRNAs is essential for maintaining the differentiated phenotype of human islet beta cells. Inhibition of miR-30 family microRNAs induces epithelial-mesenchymal transition of human pancreatic islet cells.

Interactions

NFYC has been shown to interact with Myc.

Further reading

• Mantovani R (1999). "The molecular biology of the CCAAT-binding factor NF-Y". Gene. 239 (1): 15–27. doi:10.1016/S0378-1119(99)00368-6. PMID 10571030.
• Martinelli R, Heintz N (1994). "H1TF2A, the large subunit of a heterodimeric, glutamine-rich CCAAT-binding transcription factor involved in histone H1 cell cycle regulation". Mol. Cell. Biol. 14 (12): 8322–32. doi:10.1128/mcb.14.12.8322. PMC 359371. PMID 7969168.
• Nakshatri H, Bhat-Nakshatri P, Currie RA (1997). "Subunit association and DNA binding activity of the heterotrimeric transcription factor NF-Y is regulated by cellular redox". J. Biol. Chem. 271 (46): 28784–91. doi:10.1074/jbc.271.46.28784. hdl:1805/18866. PMID 8910521.
• Dmitrenko V, Garifulin O, Kavsan V (1997). "Isolation and sequence analysis of the cDNA encoding subunit C of human CCAAT-binding transcription factor". Gene. 197 (1–2): 161–3. doi:10.1016/S0378-1119(97)00255-2. PMID 9332362.
• Currie RA (1998). "Functional interaction between the DNA binding subunit trimerization domain of NF-Y and the high mobility group protein HMG-I(Y)". J. Biol. Chem. 272 (49): 30880–8. doi:10.1074/jbc.272.49.30880. PMID 9388234.
• Taira T, Sawai M, Ikeda M, Tamai K, Iguchi-Ariga SM, Ariga H (1999). "Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y". J. Biol. Chem. 274 (34): 24270–9. doi:10.1074/jbc.274.34.24270. PMID 10446203.
• Yoshida H, Okada T, Haze K, Yanagi H, Yura T, Negishi M, Mori K (2001). "Endoplasmic reticulum stress-induced formation of transcription factor complex ERSF including NF-Y (CBF) and activating transcription factors 6alpha and 6beta that activates the mammalian unfolded protein response". Mol. Cell. Biol. 21 (4): 1239–48. doi:10.1128/MCB.21.4.1239-1248.2001. PMC 99577. PMID 11158310.
• Izumi H, Molander C, Penn LZ, Ishisaki A, Kohno K, Funa K (2001). "Mechanism for the transcriptional repression by c-Myc on PDGF beta-receptor". J. Cell Sci. 114 (Pt 8): 1533–44. doi:10.1242/jcs.114.8.1533. PMID 11282029.
• Chen F, Ogawa K, Liu X, Stringfield TM, Chen Y (2002). "Repression of Smad2 and Smad3 transactivating activity by association with a novel splice variant of CCAAT-binding factor C subunit". Biochem. J. 364 (Pt 2): 571–7. doi:10.1042/BJ20011703. PMC 1222603. PMID 12023901.
• Romier C, Cocchiarella F, Mantovani R, Moras D (2003). "The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y". J. Biol. Chem. 278 (2): 1336–45. doi:10.1074/jbc.M209635200. PMID 12401788.
• Salsi V, Caretti G, Wasner M, Reinhard W, Haugwitz U, Engeland K, Mantovani R (2003). "Interactions between p300 and multiple NF-Y trimers govern cyclin B2 promoter function". J. Biol. Chem. 278 (9): 6642–50. doi:10.1074/jbc.M210065200. hdl:11380/1202119. PMID 12482752.
• Hirose T, Sowa Y, Takahashi S, Saito S, Yasuda C, Shindo N, Furuichi K, Sakai T (2003). "p53-independent induction of Gadd45 by histone deacetylase inhibitor: coordinate regulation by transcription factors Oct-1 and NF-Y". Oncogene. 22 (49): 7762–73. doi:10.1038/sj.onc.1207091. PMID 14586402. S2CID 1826456.
• Chattopadhyay C, Hawke D, Kobayashi R, Maity SN (2004). "Human p32, interacts with B subunit of the CCAAT-binding factor, CBF/NF-Y, and inhibits CBF-mediated transcription activation in vitro". Nucleic Acids Res. 32 (12): 3632–41. doi:10.1093/nar/gkh692. PMC 484179. PMID 15243141.
• Wang B, Gao C, Ponder KP (2005). "C/EBPbeta contributes to hepatocyte growth factor-induced replication of rodent hepatocytes". J. Hepatol. 43 (2): 294–302. doi:10.1016/j.jhep.2005.02.029. PMID 15922473.

External links

• NFYC protein, human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.