NEK2

Serine/threonine-protein kinase Nek2 is an enzyme that in humans is encoded by the NEK2 gene.

NEK2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2JAV, 2W5A, 2W5B, 2WQO, 2XK3, 2XK4, 2XK6, 2XK7, 2XK8, 2XKC, 2XKD, 2XKE, 2XKF, 2XNM, 2XNN, 2XNO, 2XNP, 4A4X, 4AFE
Identifiers
Aliases	NEK2, HsPK21, NEK2A, NLK1, PPP1R111, RP67, NIMA related kinase 2
External IDs	OMIM: 604043; MGI: 109359; HomoloGene: 74441; GeneCards: NEK2; OMA:NEK2 - orthologs
Gene location (Human) Chr. Chromosome 1 (human) Band 1q32.3 Start 211,658,657 bp End 211,675,630 bp
Gene location (Mouse) Chr. Chromosome 1 (mouse) Band 1 H6|1 96.94 cM Start 191,553,556 bp End 191,565,162 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm ventricular zone secondary oocyte left testis right testis gonad ganglionic eminence testicle bone marrow trabecular bone Top expressed in spermatocyte primary oocyte spermatid zygote testicle secondary oocyte yolk sac epiblast bone marrow morula More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity protein kinase activity nucleotide binding metal ion binding kinase activity protein serine/threonine kinase activity protein binding protein phosphatase binding ATP binding Cellular component cytosol centrosome spindle pole chromosome condensed nuclear chromosome microtubule organizing center midbody nucleolus microtubule chromosome, centromeric region cytoskeleton kinetochore nucleoplasm nucleus cytoplasm protein-containing complex Biological process positive regulation of telomere capping regulation of mitotic centrosome separation phosphorylation chromosome segregation negative regulation of DNA binding positive regulation of telomere maintenance via telomerase spindle assembly cell division protein phosphorylation mitotic spindle assembly G2/M transition of mitotic cell cycle meiosis positive regulation of telomerase activity centrosome separation mitotic sister chromatid segregation protein autophosphorylation cell cycle negative regulation of centriole-centriole cohesion regulation of attachment of spindle microtubules to kinetochore blastocyst development regulation of mitotic nuclear division ciliary basal body-plasma membrane docking mitotic cell cycle regulation of G2/M transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4751 18005 Ensembl ENSG00000117650 ENSMUSG00000026622 UniProt P51955 O38632 RefSeq (mRNA) NM_001204182 NM_001204183 NM_002497 NM_010892 RefSeq (protein) NP_001191111 NP_001191112 NP_002488 NP_035022 Location (UCSC) Chr 1: 211.66 – 211.68 Mb Chr 1: 191.55 – 191.57 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Interactions

NEK2 has been shown to interact with MAPK1 and NDC80. Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not [Uniprot].

Further reading

• Hayward DG, Fry AM (2006). "Nek2 kinase in chromosome instability and cancer". Cancer Lett. 237 (2): 155–66. doi:10.1016/j.canlet.2005.06.017. PMID 16084011.
• Schultz SJ, Fry AM, Sütterlin C, Ried T, Nigg EA (1994). "Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans". Cell Growth Differ. 5 (6): 625–35. PMID 7522034.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Fry AM, Meraldi P, Nigg EA (1998). "A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators". EMBO J. 17 (2): 470–81. doi:10.1093/emboj/17.2.470. PMC 1170398. PMID 9430639.
• Fry AM, Mayor T, Meraldi P, Stierhof YD, Tanaka K, Nigg EA (1998). "C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2". J. Cell Biol. 141 (7): 1563–74. doi:10.1083/jcb.141.7.1563. PMC 2133000. PMID 9647649.
• Fry AM, Arnaud L, Nigg EA (1999). "Activity of the human centrosomal kinase, Nek2, depends on an unusual leucine zipper dimerization motif". J. Biol. Chem. 274 (23): 16304–10. doi:10.1074/jbc.274.23.16304. PMID 10347187.
• Schutte BC, Bjork BC, Coppage KB, Malik MI, Gregory SG, Scott DJ, Brentzell LM, Watanabe Y, Dixon MJ, Murray JC (2000). "A preliminary gene map for the Van der Woude syndrome critical region derived from 900 kb of genomic sequence at 1q32-q41". Genome Res. 10 (1): 81–94. doi:10.1101/gr.10.1.81 (inactive 1 November 2024). PMC 310500. PMID 10645953.{{cite journal}}: CS1 maint: DOI inactive as of November 2024 (link)
• Helps NR, Luo X, Barker HM, Cohen PT (2001). "NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1". Biochem. J. 349 (Pt 2): 509–18. doi:10.1042/0264-6021:3490509. PMC 1221174. PMID 10880350.
• Hames RS, Fry AM (2002). "Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis". Biochem. J. 361 (Pt 1): 77–85. doi:10.1042/0264-6021:3610077. PMC 1222281. PMID 11742531.
• Ha Kim Y, Yeol Choi J, Jeong Y, Wolgemuth DJ, Rhee K (2002). "Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle". Biochem. Biophys. Res. Commun. 290 (2): 730–6. doi:10.1006/bbrc.2001.6212. PMID 11785960.
• Eto M, Elliott E, Prickett TD, Brautigan DL (2003). "Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation". J. Biol. Chem. 277 (46): 44013–20. doi:10.1074/jbc.M208035200. PMID 12221103.
• Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH (2003). "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation". J. Biol. Chem. 277 (51): 49408–16. doi:10.1074/jbc.M207069200. PMID 12386167.
• Faragher AJ, Fry AM (2004). "Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles". Mol. Biol. Cell. 14 (7): 2876–89. doi:10.1091/mbc.E03-02-0108. PMC 165683. PMID 12857871.
• Lou Y, Yao J, Zereshki A, Dou Z, Ahmed K, Wang H, Hu J, Wang Y, Yao X (2004). "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling". J. Biol. Chem. 279 (19): 20049–57. doi:10.1074/jbc.M314205200. PMID 14978040.
• Noguchi K, Fukazawa H, Murakami Y, Uehara Y (2004). "Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells". J. Biol. Chem. 279 (31): 32716–27. doi:10.1074/jbc.M404104200. PMID 15161910.
• Fortna A, Kim Y, MacLaren E, Marshall K, Hahn G, Meltesen L, Brenton M, Hink R, Burgers S, Hernandez-Boussard T, Karimpour-Fard A, Glueck D, McGavran L, Berry R, Pollack J, Sikela JM (2006). "Lineage-specific gene duplication and loss in human and great ape evolution". PLOS Biol. 2 (7): E207. doi:10.1371/journal.pbio.0020207. PMC 449870. PMID 15252450.
• Lou Y, Xie W, Zhang DF, Yao JH, Luo ZF, Wang YZ, Shi YY, Yao XB (2004). "Nek2A specifies the centrosomal localization of Erk2". Biochem. Biophys. Res. Commun. 321 (2): 495–501. doi:10.1016/j.bbrc.2004.06.171. PMID 15358203.