Gambrell is a surname. Notable people with the surname include:
- Billy Gambrell (born 1941), former American football wide receiver in the National Football League
- David H. Gambrell (1929–2021), American politician and Georgia attorney who represented his state in the United States Senate from 1971 through 1972
- Dorothy Gambrell, cartoonist who writes and draws the online comic strip Cat and Girl
- Dylan Gambrell (born 1996), American ice hockey player
- James Bruton Gambrell (1841–1921), American Baptist minister
- Jamey Gambrell (1954–2020), American translator of Russian literature, and an expert in modern art
- Marilyn Gambrell, American parole officer turned teacher who started the program No More Victims at the M.B. Smiley High School in Houston, Texas
- Michael Gambrell (born 1958), American politician