Fowlkes is a surname. Notable people with the surname include:
- Alan Fowlkes (born 1958), retired professional baseball player
- Charles Fowlkes (1916–1980), American baritone saxophonist
- Curtis Fowlkes, American jazz trombonist
- David Fowlkes, Jr. (born 1970 in Milwaukee, Wisconsin), the inventor of the Spinner
- Eddie Fowlkes (born 1962), techno DJ from Detroit, Michigan
- Herman Fowlkes, Jr. (1919–1993), American jazz musician and educator from Chicago, Illinois
- Lauren Fowlkes (born 1988), American soccer player from Lee's Summit, Missouri
- Tremaine Fowlkes (born 1976), American professional basketball player