1965 U.S. National Championships – Women's singles

First-seeded Margaret Smith defeated fifth-seeded Billie Jean Moffitt 8–6, 7–5 in the final to win the women's singles tennis title at the 1965 U.S. National Championships.

Seeds

The seeded players are listed below. Margaret Smith is the champion; others show in brackets the round in which they were eliminated.

^ ^a ^b Collins, Bud (2010). The Bud Collins History of Tennis (2nd ed.). [New York City]: New Chapter Press. p. 472. ISBN 978-0942257700.

Preceded by 1965 Wimbledon Championships – Women's singles	Grand Slam women's singles	Succeeded by 1966 Australian Championships – Women's singles