File:Boidinia-Minimum Evolution-Tree.svg

Figure. Evolutionary relationships of Boidinia and Gloeopeniophorella
The evolutionary history was inferred using the Minimum Evolution method [1]. The optimal tree with the sum of branch length = 0.44296624 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [2]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Kimura 2-parameter method [3] and are in the units of the number of base substitutions per site. The ME tree was searched using the Close-Neighbor-Interchange (CNI) algorithm [4] at a search level of 2. The Neighbor-joining algorithm [5] was used to generate the initial tree. The analysis involved 17 nucleotide sequences. All positions with less than 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. There were a total of 1188 positions in the final dataset. Evolutionary analyses were conducted in MEGA6 [6]

1. Rzhetsky A. and Nei M. (1992). A simple method for estimating and testing minimum evolution trees. Molecular Biology and Evolution 9:945-967.
2. Felsenstein J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791.
3. Kimura M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111-120.
4. Nei M. and Kumar S. (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York.
5. Saitou N. and Nei M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
6. Tamura K., Stecher G., Peterson D., Filipski A., and Kumar S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution30: 2725-2729.

List with GenBank Sequences

• Boidinia_granulata AY048880
• Boidinia_propinqua AF506379
• Lactarius_volemus AF506413
• Boidinia_sp. AF506378
• Boidinia_aculeata AF506433
• Gloeopeniophorella_convolvens AF506435
• Gloeopeniophorella_laxa AF506440
• Russula_nauseosa AF506462
• Boidinia_furfuracea AF506376
• Pseudoxenasma_verrucisporum AF506426
• Hericium_americanum AF506458
• Hericium_abietis AF506456
• Gloeocystidiellum_porosum AF310099
• Lactarius_leonis AF506411
• Russula_violacea AF506465
• Lactarius_subemboratus AF506412