STX8

STX8
Identifikatori
Aliasi	STX8
Vanjski ID-jevi	OMIM: 604203 MGI: 1890156 HomoloGene: 37973 GeneCards: STX8
Lokacija gena (čovjek)
Hrom.	Hromosom 17 (čovjek)
Kraj	9,576,591 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	68,097,974 bp
Obrazac RNK ekspresije
	; ; ; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• SNAP receptor activity; • GO:0001948, GO:0016582 vezivanje za proteine; • chloride channel inhibitor activity; • syntaxin binding; • ubiquitin protein ligase binding; • SNARE binding;
Ćelijska komponenta	• integral component of membrane; • reciklirajući endosom; • Vezikula; • endozom; • late endosome; • membrana; • late endosome membrane; • integral component of plasma membrane; • lysosomal membrane; • trans-Golđijeva mreža; • early endosome; • Endoplazmatski retikulum; • perinuklearno područje citoplazme; • citosol; • SNARE complex; • phagocytic vesicle; • Endomembranski sistem;
Biološki proces	• endosome to lysosome transport; • vesicle docking; • early endosome to late endosome transport; • intracellular protein transport; • vesicle-mediated transport; • regulation of protein localization to plasma membrane; • Fuzija vezikula; • cellular response to interferon-gamma;
	Izvori:Amigo / QuickGO
Ortolozi
	9482
	58633
	ENSG00000170310
	ENSMUSG00000020903
	Q9UNK0
	O88983
	NM_004853
	NM_018768; NM_001356314; NM_001361350; NM_001361351; NM_001361352
	NP_004844
	NP_061238; NP_001343243; NP_001348279; NP_001348280; NP_001348281
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Sintaksin-8 jest protein koji je kod ljudi kodiran genom STX8 sa hromosoma 17.^[5]^[6]^[7] Sintaksin-8 direktno je u interakciji sa HECTd3 i ima sličnu subćelijsku lokalizaciju.^[8] Pokazalo se da ovaj protein formira SNARE kompleks sa sintaksinom-7, vti1b i endobrevinom. Oni funkcionišu kao mehanizam za homotipsku fuziju kasnih endosoma.^[9]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 236 aminokiselina, a molekulska težina 26.907 Da.^[7]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MAPDPWFSTY	DSTCQIAQEI	AEKIQQRNQY	ERKGEKAPKL	TVTIRALLQN
LKEKIALLKD	LLLRAVSTHQ	ITQLEGDRRQ	NLLDDLVTRE	RLLLASFKNE
GAEPDLIRSS	LMSEEAKRGA	PNPWLFEEPE	ETRGLGFDEI	RQQQQKIIQE
QDAGLDALSS	IISRQKQMGQ	EIGNELDEQN	EIIDDLANLV	ENTDEKLRNE
TRRVNMVDRK	SASCGMIMVI	LLLLVAIVVV	AVWPTN

Modelni organizmi

U proučavanju funkcije STX8 korišteni su modelni organizam. Uslovna nokaut-mišja linija zvana Stx8^{tm2a(EUCOMM)Wtsi}^[10]^[11] generirana je kao dio programa Međunarodnog konzorcija za nokaut-miševe — projekta mutageneze visoke propusnosti za generiranje i distribuciju životinjskih modela bolesti zainteresiranim naučnicima — na Institutu Wellcome Trust Sanger.^[12]^[13]^[14] Mužjaci i ženke podvrgnuti su standardiziranom fenotipskom pregledu, kako bi se utvrdili efekti delecija.^[15]^[16] Provedena su 24 testa na homozigotnim mutantnim odraslim miševima, ali nisu uočene nikakve značajne abnormalnosti.^[15]

*Stx8* fenotip nokaut-miševa
Svojstvo	Fenotip
Vijabilnost homozigota	Normal
Tjelesna težina	Normal
Anksioznost otvorenog polja	Normal
Neurološka procjena	Normal
Snaga stiska	Normal
Test vruće ploče	Normal
Dismorfologija	Normal
Indirektna kalorimetrija	Normal
Test tolerancije glukoze	Normal
Slušni odgovor moždanog stabla	Normal
DEXA	Normal
Radiografija	Normal
Tjelesna temperatura	Normal
Morfologija oka	Normal
Klinička hemija	Normal
Hematologija	Normal
Limfociti periferne krvi	Normal
Mikronukleus test	Normal
Težina srca	Normal
Histopatologija kože	Normal
Histopatologija mozga	Normal
Histopatologija oka	Normal
Salmonella infekcija	Normal^[17]
Citrobacter infekcija	Normal^[18]
Svi testovi i analize su prema:^[15]^[19]

Interakcije

Pokazalo se da STX8 međudjeluje sa membranskim proteinom 8 povezanim sa vezikulama,^[20] VTI1B^[21]^[22] i STX7.^[20]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000170310 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000020903 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Steegmaier M, Yang B, Yoo JS, Huang B, Shen M, Yu S, Luo Y, Scheller RH (Dec 1998). "Three novel proteins of the syntaxin/SNAP-25 family". The Journal of Biological Chemistry. 273 (51): 34171–9. doi:10.1074/jbc.273.51.34171. PMID 9852078.
^ Thoreau V, Bergès T, Callebaut I, Guillier-Gencik Z, Gressin L, Bernheim A, Karst F, Mornon JP, Kitzis A, Chomel JC (Apr 1999). "Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8)". Biochemical and Biophysical Research Communications. 257 (2): 577–83. doi:10.1006/bbrc.1999.0503. PMID 10198254.
^ ^a ^b "Entrez Gene: STX8 syntaxin 8".
^ Zhang L, Kang L, Bond W, Zhang N (Feb 2009). "Interaction between syntaxin 8 and HECTd3, a HECT domain ligase". Cellular and Molecular Neurobiology. 29 (1): 115–21. doi:10.1007/s10571-008-9303-0. PMID 18821010. S2CID 23216243.
^ Kasai K, Suga K, Izumi T, Akagawa K (October 2008). "Syntaxin 8 has two functionally distinct di-leucine-based motifs". Cellular & Molecular Biology Letters. 13 (1): 144–54. doi:10.2478/s11658-007-0043-9. PMC 6275627. PMID 17965969.
^ "International Knockout Mouse Consortium".
^ "Mouse Genome Informatics".
^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
^ ^a ^b ^c Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
^ "Salmonella infection data for Stx8". Wellcome Trust Sanger Institute.
^ "Citrobacter infection data for Stx8". Wellcome Trust Sanger Institute.
^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
^ ^a ^b Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF, Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". The EMBO Journal. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.
^ Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
^ Subramaniam VN, Loh E, Horstmann H, Habermann A, Xu Y, Coe J, Griffiths G, Hong W (Mar 2000). "Preferential association of syntaxin 8 with the early endosome". Journal of Cell Science. 113 ( Pt 6) (6): 997–1008. PMID 10683148.

Dopuznska literatura

Prekeris R, Yang B, Oorschot V, Klumperman J, Scheller RH (Nov 1999). "Differential roles of syntaxin 7 and syntaxin 8 in endosomal trafficking". Molecular Biology of the Cell. 10 (11): 3891–908. doi:10.1091/mbc.10.11.3891. PMC 25687. PMID 10564279.
Subramaniam VN, Loh E, Horstmann H, Habermann A, Xu Y, Coe J, Griffiths G, Hong W (Mar 2000). "Preferential association of syntaxin 8 with the early endosome". Journal of Cell Science. 113 ( Pt 6) (6): 997–1008. PMID 10683148.
McShea A, Samuel T, Eppel JT, Galloway DA, Funk JO (Jul 2000). "Identification of CIP-1-associated regulator of cyclin B (CARB), a novel p21-binding protein acting in the G2 phase of the cell cycle". The Journal of Biological Chemistry. 275 (30): 23181–6. doi:10.1074/jbc.M001772200. PMID 10781590.
Scales SJ, Chen YA, Yoo BY, Patel SM, Doung YC, Scheller RH (May 2000). "SNAREs contribute to the specificity of membrane fusion". Neuron. 26 (2): 457–64. doi:10.1016/S0896-6273(00)81177-0. PMID 10839363.
Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF, Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". The EMBO Journal. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.
Wade N, Bryant NJ, Connolly LM, Simpson RJ, Luzio JP, Piper RC, James DE (Jun 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". The Journal of Biological Chemistry. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. PMID 11278762.
Antonin W, Fasshauer D, Becker S, Jahn R, Schneider TR (Feb 2002). "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nature Structural Biology. 9 (2): 107–11. doi:10.1038/nsb746. hdl:11858/00-001M-0000-0012-F459-F. PMID 11786915. S2CID 17724790.
Bogdanovic A, Bennett N, Kieffer S, Louwagie M, Morio T, Garin J, Satre M, Bruckert F (Nov 2002). "Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum". The Biochemical Journal. 368 (Pt 1): 29–39. doi:10.1042/BJ20020845. PMC 1222979. PMID 12175335.
Xu Y, Martin S, James DE, Hong W (Oct 2002). "GS15 forms a SNARE complex with syntaxin 5, GS28, and Ykt6 and is implicated in traffic in the early cisternae of the Golgi apparatus". Molecular Biology of the Cell. 13 (10): 3493–507. doi:10.1091/mbc.E02-01-0004. PMC 129961. PMID 12388752.
Kim BY, Ueda M, Kominami E, Akagawa K, Kohsaka S, Akazawa C (Nov 2003). "Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex". Biochemical and Biophysical Research Communications. 311 (3): 577–82. doi:10.1016/j.bbrc.2003.10.030. PMID 14623309.
Zhu X, McShea A, Harris PL, Raina AK, Castellani RJ, Funk JO, Shah S, Atwood C, Bowen R, Bowser R, Morelli L, Perry G, Smith MA (Mar 2004). "Elevated expression of a regulator of the G2/M phase of the cell cycle, neuronal CIP-1-associated regulator of cyclin B, in Alzheimer's disease". Journal of Neuroscience Research. 75 (5): 698–703. doi:10.1002/jnr.20028. PMID 14991845. S2CID 39141122.
Bilan F, Thoreau V, Nacfer M, Dérand R, Norez C, Cantereau A, Garcia M, Becq F, Kitzis A (Apr 2004). "Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity". Journal of Cell Science. 117 (Pt 10): 1923–35. doi:10.1242/jcs.01070. PMID 15039462.
Barrios-Rodiles M, Brown KR, Ozdamar B, Bose R, Liu Z, Donovan RS, Shinjo F, Liu Y, Dembowy J, Taylor IW, Luga V, Przulj N, Robinson M, Suzuki H, Hayashizaki Y, Jurisica I, Wrana JL (Mar 2005). "High-throughput mapping of a dynamic signaling network in mammalian cells". Science. 307 (5715): 1621–5. doi:10.1126/science.1105776. PMID 15761153. S2CID 39457788.
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

Vanjski linkovi

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000170310 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000020903 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9852078-5] Steegmaier M, Yang B, Yoo JS, Huang B, Shen M, Yu S, Luo Y, Scheller RH (Dec 1998). "Three novel proteins of the syntaxin/SNAP-25 family". The Journal of Biological Chemistry. 273 (51): 34171–9. doi:10.1074/jbc.273.51.34171. PMID 9852078.

[pmid10198254-6] Thoreau V, Bergès T, Callebaut I, Guillier-Gencik Z, Gressin L, Bernheim A, Karst F, Mornon JP, Kitzis A, Chomel JC (Apr 1999). "Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8)". Biochemical and Biophysical Research Communications. 257 (2): 577–83. doi:10.1006/bbrc.1999.0503. PMID 10198254.

[entrez-7] "Entrez Gene: STX8 syntaxin 8".

[pmid18821010-8] Zhang L, Kang L, Bond W, Zhang N (Feb 2009). "Interaction between syntaxin 8 and HECTd3, a HECT domain ligase". Cellular and Molecular Neurobiology. 29 (1): 115–21. doi:10.1007/s10571-008-9303-0. PMID 18821010. S2CID 23216243.

[pmid17965969-9] Kasai K, Suga K, Izumi T, Akagawa K (October 2008). "Syntaxin 8 has two functionally distinct di-leucine-based motifs". Cellular & Molecular Biology Letters. 13 (1): 144–54. doi:10.2478/s11658-007-0043-9. PMC 6275627. PMID 17965969.

[allele_ref-10] "International Knockout Mouse Consortium".

[mgi_allele_ref-11] "Mouse Genome Informatics".

[pmid21677750-12] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-13] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-14] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.

[mgp_reference-15] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.

[pmid21722353-16] van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

[''Salmonella''_infection-17] "Salmonella infection data for Stx8". Wellcome Trust Sanger Institute.

[''Citrobacter''_infection-18] "Citrobacter infection data for Stx8". Wellcome Trust Sanger Institute.

[19] Mouse Resources Portal, Wellcome Trust Sanger Institute.

[pmid11101518-20] Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard GF, Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". The EMBO Journal. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.

[pmid16189514-21] Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

[pmid10683148-22] Subramaniam VN, Loh E, Horstmann H, Habermann A, Xu Y, Coe J, Griffiths G, Hong W (Mar 2000). "Preferential association of syntaxin 8 with the early endosome". Journal of Cell Science. 113 ( Pt 6) (6): 997–1008. PMID 10683148.

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