POLR3G

POLR3G
Identifikatori
Aliasi	POLR3G
Vanjski ID-jevi	OMIM: 617456 MGI: 1914736 HomoloGene: 38184 GeneCards: POLR3G
Lokacija gena (čovjek)
Hrom.	Hromosom 5 (čovjek)
Kraj	90,514,557 bp
Lokacija gena (miš)
Hrom.	Hromosom 13 (miš)
Kraj	81,859,132 bp
Ontologija gena
Molekularna funkcija	• DNA-directed 5'-3' RNA polymerase activity; • GO:0001948, GO:0016582 vezivanje za proteine;
Ćelijska komponenta	• RNA polymerase III complex; • citosol; • nukleoplazma; • jedro; • citoplazma;
Biološki proces	• positive regulation of interferon-beta production; • immune system process; • Ćelijska proliferacija; • defense response to virus; • positive regulation of innate immune response; • Urođeni imunski sistem; • regulation of transcription by RNA polymerase III; • transcription by RNA polymerase III; • positive regulation of type I interferon production;
	Izvori:Amigo / QuickGO
Ortolozi
	10622
	67486
	ENSG00000113356
	ENSMUSG00000035834
	O15318
	Q6NXY9
	NM_006467; NM_001370351; NM_001370352; NM_001370353; NM_001370354
	NM_001081176; NM_026190
	NP_006458; NP_001357280; NP_001357281; NP_001357282; NP_001357283
	NP_001074645
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Polimerazno (RNK) III (DNK-usmjereni) polipeptid G (32kD) jest protein koji je kod ljudi kodiran genom POLR3G sa hromosoma 5.^[5]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 223 aminokiselina, а molekulska težina 25.914 Da.^[6]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MAGNKGRGRA	AYTFNIEAVG	FSKGEKLPDV	VLKPPPLFPD	TDYKPVPLKT
GEGEEYMLAL	KQELRETMKR	MPYFIETPEE	RQDIERYSKR	YMKVYKEEWI
PDWRRLPREM	MPRNKCKKAG	PKPKKAKDAG	KGTPLTNTED	VLKKMEELEK
RGDGEKSDEE	NEEKEGSKEK	SKEGDDDDDD	DAAEQEEYDE	EEQEEENDYI
NSYFEDGDDF	GADSDDNMDE	ATY

Modenil organizmi

U proučavanju funkcije POLR3G korišteni su modelni organizmi. Uvjetna nokaut-mišja linija pod nazivom Polr3g^{tm1a (EUCOMM) Wtsi} generirana je na Institutu Wellcome Trust Sanger.^[7] Mužjaci i ženke podvrgnuti su standardiziranom fenotipskom pregledu^[8] to determine the effects of deletion.^[9]^[10]^[11]^[12] Dodatni pregledi obavljeni su dubinskom imunološkom fenotipizacijom.^[13] Od 23 analizirana pokazatelja, ozbiljnije nenormalnosti nađene su u samo dva slučaja.

Fenotup *Polr3g* nokaut-miševa
Svojstvo	Fenotip
Svi podaci raspoloživi na linku:^[8]^[13]
Insulin	Normalan
Vijabilnost homozigota na P14	Nenormalan
Studija recesivne letalnosti	Nenormalan
Tjelesna težina	Normalan
Neurološka procjena	Normalan
Jačina stiska	Normalan
Dismorfologija	Normalan
Indirektna kalorimetrija	Normalan
Test tolerancije glukoze	Normalan
Slušni odgovor moždanog stabla	Normalan
DEXA	Normalan
Radiografija	Normalan
Morfologija oka	Normalan
Klinička hemija	Normalan
Hematologija 16 sedmica	Normalan
Leukociti periferne krvi 16 sedmica	Normalan
Težina srca	Normalan
Funkcija citotoksičnih T-ćelija	Normalan
Imunofenotipizacija slezene	Normalan
Imunofenotipizacija mezenternog limfnog čvora	Normalan
Imunofenotipizacija koštane srži	Normalan
Sastav epidermne imunosti	Normalan
Izazov gripe	Normalan

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000113356 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000035834 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Entrez Gene: Polymerase (RNA) III (DNA directed) polypeptide G (32kD)". Pristupljeno 1. 4. 2014.
^ "UniProt, O15318" (jezik: engleski). Pristupljeno 19. 10. 2021.
^ Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
^ ^a ^b "International Mouse Phenotyping Consortium".
^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
^ White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
^ ^a ^b "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 19. 10. 2021.

Dopunska literatura

Ablasser, A; Bauernfeind, F; Hartmann, G; Latz, E; Fitzgerald, K. A.; Hornung, V (2009). "RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate" (PDF). Nature Immunology. 10 (10): 1065–72. doi:10.1038/ni.1779. PMC 3878616. PMID 19609254.
Chiu, Y. H.; MacMillan, J. B.; Chen, Z. J. (2009). "RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway". Cell. 138 (3): 576–91. doi:10.1016/j.cell.2009.06.015. PMC 2747301. PMID 19631370.
Chantarangsu, S; Mushiroda, T; Mahasirimongkol, S; Kiertiburanakul, S; Sungkanuparph, S; Manosuthi, W; Tantisiriwat, W; Charoenyingwattana, A; Sura, T; Takahashi, A; Kubo, M; Kamatani, N; Chantratita, W; Nakamura, Y (2011). "Genome-wide association study identifies variations in 6p21.3 associated with nevirapine-induced rash". Clinical Infectious Diseases. 53 (4): 341–8. doi:10.1093/cid/cir403. PMID 21810746.
Wong, R. C.; Pollan, S; Fong, H; Ibrahim, A; Smith, E. L.; Ho, M; Laslett, A. L.; Donovan, P. J. (2011). "A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells". Stem Cells. 29 (10): 1517–27. doi:10.1002/stem.714. PMID 21898682. S2CID 24305950.
Jang, K. L.; Collins, M. K.; Latchman, D. S. (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". Journal of Acquired Immune Deficiency Syndromes. 5 (11): 1142–7. PMID 1403646.
Wang, Z; Roeder, R. G. (1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes & Development. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.
Hu, P; Wu, S; Sun, Y; Yuan, C. C.; Kobayashi, R; Myers, M. P.; Hernandez, N (2002). "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits". Molecular and Cellular Biology. 22 (22): 8044–55. doi:10.1128/MCB.22.22.8044-8055.2002. PMC 134740. PMID 12391170.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000113356 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000035834 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: Polymerase (RNA) III (DNA directed) polypeptide G (32kD)". Pristupljeno 1. 4. 2014.

[UniProt-6] "UniProt, O15318" (jezik: engleski). Pristupljeno 19. 10. 2021.

[mgp_reference-7] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.

[IMPCsearch_ref-8] "International Mouse Phenotyping Consortium".

[pmid21677750-9] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-10] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-11] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.

[pmid23870131-12] White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.

[iii_ref-13] "Infection and Immunity Immunophenotyping (3i) Consortium". Arhivirano s originala, 21. 5. 2015. Pristupljeno 19. 10. 2021.

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