CIAPIN1

CIAPIN1
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	2LD4, 2YUI, 4M7R
Identifikatori
Aliasi	CIAPIN1
Vanjski ID-jevi	OMIM: 608943 MGI: 1922083 HomoloGene: 10658 GeneCards: CIAPIN1
Lokacija gena (čovjek)
Hrom.	Hromosom 16 (čovjek)
Kraj	57,447,420 bp
Lokacija gena (miš)
Hrom.	Hromosom 8 (miš)
Kraj	95,564,986 bp
Ontologija gena
Molekularna funkcija	• GO:0102674, GO:0102675 methyltransferase activity; • iron-sulfur cluster binding; • GO:0001948, GO:0016582 vezivanje za proteine; • vezivanje iona metala; • 2 iron, 2 sulfur cluster binding; • 4 iron, 4 sulfur cluster binding; • electron transfer activity;
Ćelijska komponenta	• citoplazma; • mitochondrial intermembrane space; • Jedarce; • jedro; • nukleoplazma; • mitohondrija;
Biološki proces	• Metilacija; • metabolizam; • negative regulation of apoptotic process; • iron-sulfur cluster assembly; • GO:0097285 apoptoza; • Hematopoeza; • Lanac transporta elektrona;
	Izvori:Amigo / QuickGO
Ortolozi
	57019
	109006
	ENSG00000005194
	ENSMUSG00000031781
	Q6FI81
	Q8WTY4
	NM_001308347; NM_001308358; NM_020313
	NM_134141
	NP_001295276; NP_001295287; NP_064709
	NP_598902
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Anamorzin je protein koji je kod ljudi kodsiran genom CIAPIN1.^[5]^[6]^[7]

CIAPIN1 je citokinski inducirani inhibitor apoptoza bez veze sa njenim regulatornim molekulama iz porodica BCL2 (MIM 151430) ili CASP (vidi MIM 147678) . Ekspresija CIAPIN1 ovisi o stimulaciji faktor rasta (Shibayama et al., 2004).^[7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 312 aminokiselina, a molekulska težina 33.582 Da.^[8]

10	20	30	40	50
MADFGISAGQ	FVAVVWDKSS	PVEALKGLVD	KLQALTGNEG	RVSVENIKQL
LQSAHKESSF	DIILSGLVPG	STTLHSAEIL	AEIARILRPG	GCLFLKEPVE
TAVDNNSKVK	TASKLCSALT	LSGLVEVKEL	QREPLTPEEV	QSVREHLGHE
SDNLLFVQIT	GKKPNFEVGS	SRQLKLSITK	KSSPSVKPAV	DPAAAKLWTL
SANDMEDDSM	DLIDSDELLD	PEDLKKPDPA	SLRAASCGEG	KKRKACKNCT
CGLAEELEKE	KSREQMSSQP	KSACGNCYLG	DAFRCASCPY	LGMPAFKPGE
KVLLSDSNLH	DA

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Korištenjem klonirane ekspresije s IL3, neovisnom podlinijom IL3-ovisne mišje ćelijske linije, Shibayama et al. (2004) dobili su cDNK koja kodira ciapin-1, koju su nazvali anamorzin, što na latinskom znači "molekula protiv smrti". Pretražujući baze podataka, Shibayama et al. (2004) identificirali su ljudski anamorzin. Predviđeni mišji protein s 310 aminokiselina ima izračunatu molekulsku masu od 37 kD i sadrži motiv N-terminalne metiltransferaze. Anamorzin čovjeka i miša dijeli 82% identiteta aminokiselina. Northern blot analiza ljudskih tkiva otkrila je sveprisutnu ekspresiju, sa posebno visokim nivoima u srcu, jetri i gušterači. Imunofluorescentna mikroskopija mišjih ćelija pokazala je da je anamorzin lokalizovan isključivo u citoplazmi.

Funkcija

Shibayama et al. (2004) otkrili su da ekspresija anamorzina u mišjim ćelijama daje otpor apoptozi uzrokovanoj lišavanjem IL3. Dodavanje faktora rasta, kao što su EPO, SCF, TPO ili IL3, koji svi ovise o RAS signalizaciji, inducira o dozi ovisnu ekspresiju anamorzina u ćelijama miša. Na osnovu ovih rezultata i njihovih nalaza kod null miševa, zaključili su da je anamorzin presudan u hematopoezi i u posredovanju antiapoptoze izazvane citokinima16.

Životinjski model

Shibayama et al. (2004) generirali su mišji anamorzin null, a miševi su svi uginuli u kasnoj gestaciji, zbog neispravne hematopoeze u fetusnoj jetri. Kod ovih miševa poremećeno je formiranje mijeloidne, a posebno eritroidne kolonije. Analiza mikromreža otkrila je smanjenu ekspresiju Bclxl i Jak2 u fetusnoj jetri null miševa.^[9]^[10]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000005194 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000031781 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Loftus BJ, Kim UJ, Sneddon VP, Kalush F, Brandon R, Fuhrmann J, Mason T, Crosby ML, Barnstead M, Cronin L, Deslattes Mays A, Cao Y, Xu RX, Kang HL, Mitchell S, Eichler EE, Harris PC, Venter JC, Adams MD (Nov 1999). "Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q". Genomics. 60 (3): 295–308. doi:10.1006/geno.1999.5927. PMID 10493829.
^ Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Bocher M, Blocker H, Bauersachs S, Blum H, Lauber J, Dusterhoft A, Beyer A, Kohrer K, Strack N, Mewes HW, Ottenwalder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (Mar 2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
^ ^a ^b "Entrez Gene: CIAPIN1 cytokine induced apoptosis inhibitor 1".
^ "UniProt, Q6FI81". Pristupljeno 3. 7. 2021.
^ .Loftus, B. J., Kim, U.-J., Sneddon, V. P., Kalush, F., Brandon, R., Fuhrmann, J., Mason, T., Crosby, M. L., Barnstead, M., Cronin, L., Deslattes Mays, A., Cao, Y., Xu, R. X., Kang, H.-L., Mitchell, S., Eichler, E. E., Harris, P. C., Venter, J. C., Adams, M. D. Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q. Genomics 60: 295-308, 1999. PubMed: 10493829
^ Shibayama, H., Takai, E., Matsumura, I., Kouno, M., Morii, E., Kitamura, Y., Takeda, J., Kanakura, Y. Identification of a cytokine-induced antiapoptotic molecule Anamorsin essential for definitive hematopoiesis. J. Exp. Med. 199: 581-592, 2004. PubMed: 14970183

Vanjski linkovi

Lokacija ljudskog genoma CIAPIN1 i stranica sa detaljima o genu CIAPIN1 u UCSC Genome Browseru.

Dopunska literatura

Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Shibayama H, Takai E, Matsumura I, et al. (2004). "Identification of a Cytokine-induced Antiapoptotic Molecule Anamorsin Essential for Definitive Hematopoiesis". J. Exp. Med. 199 (4): 581–92. doi:10.1084/jem.20031858. PMC 2211823. PMID 14970183.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
Hao Z, Li X, Qiao T, et al. (2006). "CIAPIN1 confers multidrug resistance by upregulating the expression of MDR-1 and MRP-1 in gastric cancer cells". Cancer Biol. Ther. 5 (3): 261–6. doi:10.4161/cbt.5.3.2381. PMID 16410721.
Hao Z, Li X, Qiao T, et al. (2007). "Subcellular localization of CIAPIN1". J. Histochem. Cytochem. 54 (12): 1437–44. doi:10.1369/jhc.6A6960.2006. PMC 3958118. PMID 16957168.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000005194 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000031781 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10493829-5] Loftus BJ, Kim UJ, Sneddon VP, Kalush F, Brandon R, Fuhrmann J, Mason T, Crosby ML, Barnstead M, Cronin L, Deslattes Mays A, Cao Y, Xu RX, Kang HL, Mitchell S, Eichler EE, Harris PC, Venter JC, Adams MD (Nov 1999). "Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q". Genomics. 60 (3): 295–308. doi:10.1006/geno.1999.5927. PMID 10493829.

[pmid11230166-6] Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Bocher M, Blocker H, Bauersachs S, Blum H, Lauber J, Dusterhoft A, Beyer A, Kohrer K, Strack N, Mewes HW, Ottenwalder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (Mar 2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.

[entrez-7] "Entrez Gene: CIAPIN1 cytokine induced apoptosis inhibitor 1".

[UniProt-8] "UniProt, Q6FI81". Pristupljeno 3. 7. 2021.

[9] .Loftus, B. J., Kim, U.-J., Sneddon, V. P., Kalush, F., Brandon, R., Fuhrmann, J., Mason, T., Crosby, M. L., Barnstead, M., Cronin, L., Deslattes Mays, A., Cao, Y., Xu, R. X., Kang, H.-L., Mitchell, S., Eichler, E. E., Harris, P. C., Venter, J. C., Adams, M. D. Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q. Genomics 60: 295-308, 1999. PubMed: 10493829

[10] Shibayama, H., Takai, E., Matsumura, I., Kouno, M., Morii, E., Kitamura, Y., Takeda, J., Kanakura, Y. Identification of a cytokine-induced antiapoptotic molecule Anamorsin essential for definitive hematopoiesis. J. Exp. Med. 199: 581-592, 2004. PubMed: 14970183

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