This repository is designed to aid in the installation and running of single-cell RNA sequencing alignment protocols and to provide a starting point for advanced downstream analysis of scRNA-seq data.
- 📁 data-processing contains instructions for data alignment using a standard
Cell Ranger
protocol andkallisto
pseudo-alignment protocol. To be added isVelocyto
a toolkit that can be used for RNA velocity analysis. - 📁 analysis contains the analysis code with some details regarding usage (NOTE: this section is very much still under development).
Outside of this repository, we also have a repository called, scrna-scripts which contains all the scripts and information for running preliminary analysis on a Cell Ranger
aligned dataset. (NOTE: Still underdevelopment – expected to be finalized the week of 11/27/2023)