3 Issues closed by 3 people

• filterAndTrim yields more valid reads when filtering paired forward and reverse reads than when filtering the reverse reads alone.

  #2067 closed Jan 8, 2025

• issue with filterAndTrim() function v1.30.0 and v1.32.0

  #2071 closed Jan 7, 2025

• Verifying presence and orientation of ITS primers

  #2068 closed Jan 7, 2025

4 Issues opened by 2 people

• Explaining discrepancies in microbiome composition caused by changes in sequence files and denoising methods.

  #2075 opened Jan 13, 2025

• R Encountering Issues When Processing PacBio Revio 16S Data with the DADA2 Pipeline

  #2074 opened Jan 9, 2025

• Following ITS dada2 cutadapt trimming, why is a minimum length of 50 used?

  #2073 opened Jan 8, 2025

• What do the filtN files do?

  #2072 opened Jan 8, 2025

4 Unresolved conversations

Sometimes conversations happen on old items that aren’t yet closed. Here is a list of all the Issues and Pull Requests with unresolved conversations.

• assignTaxonomic() never ends with UNITE reference dataset

  #2069 commented on Jan 9, 2025 • 0 new comments

• Use of priors with ITS data

  #1599 commented on Jan 9, 2025 • 0 new comments

• missing upper taxonomic rank although genus/species are detected in SILVA 138.

  #2070 commented on Jan 13, 2025 • 0 new comments

• Problem with long vectors using Pacbio long reads

  #2066 commented on Jan 13, 2025 • 0 new comments