The As0.output.json file was created with the following command:

repp make seq -i As0.input.fa -o As0.output.json --dbs addgene --settings twist.yaml -v

Which approximates to asking REPP to:

• make a plasmid sequence that's in As0.input.fa
• output build instructions in JSON to As0.output.json
• use source fragments from Addgene
• use synthesis settings from twist.yaml
• be verbose during the build