Wikipedia

WH1 domain

WH1 domains, also known as EVH1 domains, are evolutionary conserved protein domains found on WASP (VASP) proteins, which are often involved in actin polymerization.[1][2]

WH1 domain
Identifiers
SymbolWH1
PfamPF00568
InterProIPR000697
SMARTWH1
SCOP21evh / SCOPe / SUPFAM
CDDcd01205
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

Function

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WH1 domains are important for all cellular processes involving actin, this includes cell motility, cell trafficking, cell division and cytokinesis, cell signalling, and the establishment and maintenance of cell junctions and cell shape.[2]

Structure

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Tertiary structure of the WH1 domain of the Mena protein revealed structure similarities with the pleckstrin (PH) domain. The overall fold consists of a compact parallel beta-sandwich, closed along one edge by a long alpha helix. A highly conserved cluster of three surface-exposed aromatic side-chains forms the recognition site for the molecule's target ligands.[3]

Interactions

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The WASP protein family control actin polymerization by activating the Arp2/3 complex. WASP is defective in Wiskott–Aldrich syndrome (WAS) whereby in most patient cases, the majority of point mutations occur within the N-terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homologue.[4][5]

WASP family proteins contain an EVH1 (WH1) in their N-terminals which bind proline-rich sequences in the WASP interacting protein. Proteins of the RanBP1 family contain a WH1 domain in their N-terminal region, which seems to bind a different sequence motif present in the C-terminal part of RanGTP protein.[6][7]

Tertiary structure of the WH1 domain of the Mena protein revealed structure similarities with the pleckstrin homology (PH) domain. The overall fold consists of a compact parallel beta-sandwich, closed along one edge by a long alpha-helix. A highly conserved cluster of three surface-exposed aromatic side-chains forms the recognition site for the molecules target ligands.[3]

EVH1

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EVH1
 
structure of the n-wasp evh1 domain-wip complex
Identifiers
SymbolEVH1
PfamPF00568
Pfam clanCL0266
InterProIPR000697
SMARTWH1
SCOP21evh / SCOPe / SUPFAM
CDDcd00837
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

A subset of WH1 domains has been termed the EVH1 domain bind a polyproline motif. The EVH1 domain (also known as the WH1, RanBP1-WASP, or enabled/VASP homology 1 domain) is also an evolutionary conserved protein domain. EVH1 domains recognise and bind the proline-rich motif FPPPP with low-affinity, further interactions then form between flanking residues.[5][8]

The EVH1 domain is found in multi-domain Ena/Vasp homology proteins implicated in a diverse range of signalling, nuclear transport and cytoskeletal events. This domain of around 115 amino acids is present in species ranging from yeast to mammals. Many EVH1-containing proteins associate with actin-based structures and play a role in cytoskeletal organisation. EVH1 domains recognise and bind the proline-rich motif FPPPP with low-affinity, further interactions then form between flanking residues.[8][9][10]

Examples

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Human genes encoding proteins containing the WH1 (EVH1) domain include:

References

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  1. ^ Symons M, Derry JM, Karlak B, Jiang S, Lemahieu V, Mccormick F, Francke U, Abo A (March 1996). "Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization". Cell. 84 (5): 723–34. doi:10.1016/S0092-8674(00)81050-8. PMID 8625410. S2CID 17838931.723-34&rft.date=1996-03&rft_id=https://api.semanticscholar.org/CorpusID:17838931#id-name=S2CID&rft_id=info:pmid/8625410&rft_id=info:doi/10.1016/S0092-8674(00)81050-8&rft.aulast=Symons&rft.aufirst=M&rft.au=Derry, JM&rft.au=Karlak, B&rft.au=Jiang, S&rft.au=Lemahieu, V&rft.au=Mccormick, F&rft.au=Francke, U&rft.au=Abo, A&rft_id=https://doi.org/10.1016%2FS0092-8674%2800%2981050-8&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
  2. ^ a b Veltman DM, Insall RH (2010). "WASP family proteins: their evolution and its physiological implications". Mol Biol Cell. 21 (16): 2880–93. doi:10.1091/mbc.E10-04-0372. PMC 2921111. PMID 20573979.2880-93&rft.date=2010&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921111#id-name=PMC&rft_id=info:pmid/20573979&rft_id=info:doi/10.1091/mbc.E10-04-0372&rft.aulast=Veltman&rft.aufirst=DM&rft.au=Insall, RH&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921111&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
  3. ^ a b Prehoda KE, Lee DJ, Lim WA (May 1999). "Structure of the enabled/VASP homology 1 domain-peptide complex: a key component in the spatial control of actin assembly". Cell. 97 (4): 471–80. doi:10.1016/S0092-8674(00)80757-6. PMID 10338211. S2CID 17078939.471-80&rft.date=1999-05&rft_id=https://api.semanticscholar.org/CorpusID:17078939#id-name=S2CID&rft_id=info:pmid/10338211&rft_id=info:doi/10.1016/S0092-8674(00)80757-6&rft.aulast=Prehoda&rft.aufirst=KE&rft.au=Lee, DJ&rft.au=Lim, WA&rft_id=https://doi.org/10.1016%2FS0092-8674%2800%2980757-6&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
  4. ^ Ponting CP, Phillips C (1997). "Identification of homer as a homologue of the Wiskott-Aldrich syndrome protein suggests a receptor-binding function for WH1 domains". J. Mol. Med. 75 (11–12): 769–71. doi:10.1007/s001090050166. PMID 9428607. S2CID 39958754.11–12&rft.pages=769-71&rft.date=1997&rft_id=https://api.semanticscholar.org/CorpusID:39958754#id-name=S2CID&rft_id=info:pmid/9428607&rft_id=info:doi/10.1007/s001090050166&rft.aulast=Ponting&rft.aufirst=CP&rft.au=Phillips, C&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
  5. ^ a b Niebuhr K, Ebel F, Frank R, Reinhard M, Domann E, Carl UD, Walter U, Gertler FB, Wehland J, Chakraborty T (September 1997). "A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family". EMBO J. 16 (17): 5433–44. doi:10.1093/emboj/16.17.5433. PMC 1170174. PMID 9312002.5433-44&rft.date=1997-09&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170174#id-name=PMC&rft_id=info:pmid/9312002&rft_id=info:doi/10.1093/emboj/16.17.5433&rft.aulast=Niebuhr&rft.aufirst=K&rft.au=Ebel, F&rft.au=Frank, R&rft.au=Reinhard, M&rft.au=Domann, E&rft.au=Carl, UD&rft.au=Walter, U&rft.au=Gertler, FB&rft.au=Wehland, J&rft.au=Chakraborty, T&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170174&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
  6. ^ Callebaut I, Cossart P, Dehoux P (December 1998). "EVH1/WH1 domains of VASP and WASP proteins belong to a large family including Ran-binding domains of the RanBP1 family". FEBS Lett. 441 (2): 181–5. doi:10.1016/S0014-5793(98)01541-5. PMID 9883880. S2CID 8527080.181-5&rft.date=1998-12&rft_id=https://api.semanticscholar.org/CorpusID:8527080#id-name=S2CID&rft_id=info:pmid/9883880&rft_id=info:doi/10.1016/S0014-5793(98)01541-5&rft.aulast=Callebaut&rft.aufirst=I&rft.au=Cossart, P&rft.au=Dehoux, P&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
  7. ^ Beddow AL, Richards SA, Orem NR, Macara IG (April 1995). "The Ran/TC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif". Proc. Natl. Acad. Sci. U.S.A. 92 (8): 3328–32. doi:10.1073/pnas.92.8.3328. PMC 42159. PMID 7724562.3328-32&rft.date=1995-04&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC42159#id-name=PMC&rft_id=info:pmid/7724562&rft_id=info:doi/10.1073/pnas.92.8.3328&rft.aulast=Beddow&rft.aufirst=AL&rft.au=Richards, SA&rft.au=Orem, NR&rft.au=Macara, IG&rft_id=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC42159&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
  8. ^ a b Ball LJ, Jarchau T, Oschkinat H, Walter U (February 2002). "EVH1 domains: structure, function and interactions". FEBS Lett. 513 (1): 45–52. doi:10.1016/S0014-5793(01)03291-4. PMID 11911879. S2CID 10368115.45-52&rft.date=2002-02&rft_id=https://api.semanticscholar.org/CorpusID:10368115#id-name=S2CID&rft_id=info:pmid/11911879&rft_id=info:doi/10.1016/S0014-5793(01)03291-4&rft.aulast=Ball&rft.aufirst=LJ&rft.au=Jarchau, T&rft.au=Oschkinat, H&rft.au=Walter, U&rft_id=https://doi.org/10.1016%2FS0014-5793%2801%2903291-4&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
  9. ^ Pawson T. "EVH1 Domain". The Pawson Lab. Retrieved 2011-06-09.
  10. ^ PDB: 1QC6​; Fedorov AA, Fedorov E, Gertler F, Almo SC (July 1999). "Structure of EVH1, a novel proline-rich ligand-binding module involved in cytoskeletal dynamics and neural function". Nat. Struct. Biol. 6 (7): 661–5. doi:10.1038/10717. PMID 10404224. S2CID 22881412.661-5&rft.date=1999-07&rft_id=https://api.semanticscholar.org/CorpusID:22881412#id-name=S2CID&rft_id=info:pmid/10404224&rft_id=info:doi/10.1038/10717&rft.aulast=Fedorov&rft.aufirst=AA&rft.au=Fedorov, E&rft.au=Gertler, F&rft.au=Almo, SC&rfr_id=info:sid/en.wikipedia.org:WH1 domain" class="Z3988">
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This article incorporates text from the public domain Pfam and InterPro: IPR000697
This article incorporates text from the public domain Pfam and InterPro: IPR007875
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