Phosphatidate cytidylyltransferase (EC 2.7.7.41)[1][2][3] (also known as CDP- diacylglycerol synthase) (CDS) is the enzyme that catalyzes the synthesis of CDP-diacylglycerol from cytidine triphosphate and phosphatidate.
| phosphatidate cytidylyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.7.7.41 | ||||||||
| CAS no. | 9067-83-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
| Cytidylyltransferase family | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | CTP_transf_1 | ||||||||
| Pfam | PF01148 | ||||||||
| InterPro | IPR000374 | ||||||||
| PROSITE | PDOC01019 | ||||||||
| OPM superfamily | 414 | ||||||||
| OPM protein | 4q2e | ||||||||
| |||||||||
- CTP phosphatidate diphosphate CDP-diacylglycerol
Thus, the two substrates of this enzyme are cytidine triphosphate, or CTP, and phosphatidate, whereas its two products are diphosphate and CDP-diacylglycerol.
CDP-diacylglycerol is an important branch point intermediate in both prokaryotic and eukaryotic organisms. CDS is a membrane-bound enzyme.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). The systematic name of this enzyme class is CTP:phosphatidate cytidylyltransferase. Other names in common use include CDP diglyceride pyrophosphorylase, CDP-diacylglycerol synthase, CDP-diacylglyceride synthetase, cytidine diphosphoglyceride pyrophosphorylase, phosphatidate cytidyltransferase, phosphatidic acid cytidylyltransferase, CTP:1,2-diacylglycerophosphate-cytidyl transferase, CTP-diacylglycerol synthetase, DAG synthetase, and CDP-DG. This enzyme participates in glycerophospholipid metabolism and phosphatidylinositol signaling system.
References
edit- ^ Sparrow CP, Raetz CR (1985). "Purification and properties of the membrane-bound CDP-diglyceride synthetase from Escherichia coli". Journal of Biological Chemistry. 260 (22): 12084–12091. doi:10.1016/S0021-9258(17)38989-5. PMID 2995359.12084-12091&rft.date=1985&rft_id=info:doi/10.1016/S0021-9258(17)38989-5&rft_id=info:pmid/2995359&rft.aulast=Sparrow&rft.aufirst=CP&rft.au=Raetz, CR&rft_id=https://doi.org/10.1016%2FS0021-9258%2817%2938989-5&rfr_id=info:sid/en.wikipedia.org:Phosphatidate cytidylyltransferase" class="Z3988">
- ^ Shen H, Heacock PN, Clancey CJ, Dowhan W (1996). "The CDS1 gene encoding CDP-diacylglycerol synthase in Saccharomyces cerevisiae is essential for cell growth". Journal of Biological Chemistry. 271 (2): 789–795. doi:10.1074/jbc.271.2.789. PMID 8557688.789-795&rft.date=1996&rft_id=info:doi/10.1074/jbc.271.2.789&rft_id=info:pmid/8557688&rft.aulast=Shen&rft.aufirst=H&rft.au=Heacock, PN&rft.au=Clancey, CJ&rft.au=Dowhan, W&rft_id=https://doi.org/10.1074%2Fjbc.271.2.789&rfr_id=info:sid/en.wikipedia.org:Phosphatidate cytidylyltransferase" class="Z3988">
- ^ Kondo H, Goto K, Saito S, Tonosaki A (1997). "Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain". Journal of Biological Chemistry. 272 (14): 9503–9509. doi:10.1074/jbc.272.14.9503. PMID 9083091.9503-9509&rft.date=1997&rft_id=info:doi/10.1074/jbc.272.14.9503&rft_id=info:pmid/9083091&rft.aulast=Kondo&rft.aufirst=H&rft.au=Goto, K&rft.au=Saito, S&rft.au=Tonosaki, A&rft_id=https://doi.org/10.1074%2Fjbc.272.14.9503&rfr_id=info:sid/en.wikipedia.org:Phosphatidate cytidylyltransferase" class="Z3988">
- Carter JR, Kennedy EP (1966). "Enzymatic synthesis of cytidine diphosphate diglyceride". Journal of Lipid Research. 7 (5): 678–83. doi:10.1016/S0022-2275(20)39250-6. PMID 4291255.678-83&rft.date=1966&rft_id=info:doi/10.1016/S0022-2275(20)39250-6&rft_id=info:pmid/4291255&rft.aulast=Carter&rft.aufirst=JR&rft.au=Kennedy, EP&rft_id=https://doi.org/10.1016%2FS0022-2275%2820%2939250-6&rfr_id=info:sid/en.wikipedia.org:Phosphatidate cytidylyltransferase" class="Z3988">
- McCaman RE, Finnerty WR (1968). "Biosynthesis of cytidine diphosphate-diglyceride by a particulate fracgion from Micrococcus cerificans". Journal of Biological Chemistry. 243 (19): 5074–80. doi:10.1016/S0021-9258(18)91993-9. PMID 5679981.5074-80&rft.date=1968&rft_id=info:doi/10.1016/S0021-9258(18)91993-9&rft_id=info:pmid/5679981&rft.aulast=McCaman&rft.aufirst=RE&rft.au=Finnerty, WR&rft_id=https://doi.org/10.1016%2FS0021-9258%2818%2991993-9&rfr_id=info:sid/en.wikipedia.org:Phosphatidate cytidylyltransferase" class="Z3988">
- Petzold GL, Agranoff BW (1967). "The biosynthesis of cytidine diphosphate diglyceride by embryonic chick brain". Journal of Biological Chemistry. 242 (6): 1187–91. doi:10.1016/S0021-9258(18)96162-4. PMID 6067194.1187-91&rft.date=1967&rft_id=info:doi/10.1016/S0021-9258(18)96162-4&rft_id=info:pmid/6067194&rft.aulast=Petzold&rft.aufirst=GL&rft.au=Agranoff, BW&rft_id=https://doi.org/10.1016%2FS0021-9258%2818%2996162-4&rfr_id=info:sid/en.wikipedia.org:Phosphatidate cytidylyltransferase" class="Z3988">
