Nb.BbvCI is a nicking endonuclease used to cut one strand of double-stranded DNA. It has been successfully used to incorporate fluorochrome-labeled nucleotides into specific spots of a DNA sequence via nick translation.[1]
References
edit- ^ Jo K, Dhingra DM, Odijk T, de Pablo JJ, Graham MD, Runnheim R, Forrest D, Schwartz DC (2007). "A single-molecule barcoding system using nanoslits for DNA analysis". PNAS. 104 (8): 2673–8. Bibcode:2007PNAS..104.2673J. doi:10.1073/pnas.0611151104. PMC 1815240. PMID 17296933.
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