Lichenase

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Licheninase
Licheninase
Identifiers
EC no.	3.2.1.73
CAS no.	37288-51-0
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
PMC
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NCBI	proteins

Lichenase (EC 3.2.1.73, licheninase, β-(1→4)-D-glucan 4-glucanohydrolase, 1,3, 1,4-β-glucan endohydrolase, 1,3, 1,4-β-glucan 4-glucanohydrolase, 1,3-1,4-β-D-glucan 4-glucanohydrolase) is an enzyme with systematic name (1→3)-(1→4)-β-D-glucan 4-glucanohydrolase.^[1]^[2] It was named after its activity in on lichenin (a form of mixed-linkage glucan).

Activity

This enzyme catalyses hydrolysis of β-(1,4)-D-glucosidic linkages in mixed-linkage glucans containing both (1,3)- and (1,4)-bonds

Specificity

The best-characterised variant of this of enzyme is Bacillus subtilis lichenase, which is used as a molecular biology tool in determining the structure of mixed-linkage glucans.^[3]^[4]^[5]^[6] This variant cleaves (1,4) bonds that immediately follow a (1,3) bond.^[7]

Other lichenases have different specificities, for example Aspergillus japonicus lichenase cleaves (1,4) bonds that immediately precede a (1,3) bond.^[8]

Structure

Lichenases are from glycoside hydrolase family 16, and share a jellyroll structure.^[9]^[10]^[11] A deep surface cleft acts as the substrate binding site.^[11]

References

^ Barras DR, Moore AE, Stone BA (1969). "Enzyme-Substrate Relationships Among β-Glucan Hydrolases". Cellulases and Their Applications. Advances in Chemistry. Vol. 95. pp. 105–138. doi:10.1021/ba-1969-0095.ch008. ISBN 0-8412-0095-5.
^ "Lichenase endo-1-3-1-4-beta-D-Glucanase Bacillus subtilis". megazyme.com. Retrieved 2019-06-25.
^ McCleary, Barry V; Codd, Rachel (1991). "Measurement of (1 → 3),(1 → 4)-β-D-glucan in barley and oats: A streamlined enzymic procedure". Journal of the Science of Food and Agriculture. 55 (2): 303–312. Bibcode:1991JSFA...55..303M. doi:10.1002/jsfa.2740550215. ISSN 0022-5142.
^ Mangan, D.; Liadova, A.; Ivory, R.; McCleary, B. V. (2016-11-29). "Novel approaches to the automated assay of β-glucanase and lichenase activity". Carbohydrate Research. 435: 162–172. doi:10.1016/j.carres.2016.10.006. ISSN 0008-6215. PMID 27810709.
^ Yoo, Dong-Hyung; Lee, Byung-Hoo; Chang, Pahn-Shick; Lee, Hyeon Gyu; Yoo, Sang-Ho (2007-03-01). "Improved Quantitative Analysis of Oligosaccharides from Lichenase-Hydrolyzed Water-Soluble Barley β-Glucans by High-Performance Anion-Exchange Chromatography". Journal of Agricultural and Food Chemistry. 55 (5): 1656–1662. Bibcode:2007JAFC...55.1656Y. doi:10.1021/jf062603l. ISSN 0021-8561. PMID 17284049.
^ Hrmova, Maria; Fincher, Geoffrey B. (2009-01-01), Bacic, Antony; Fincher, Geoffrey B.; Stone, Bruce A. (eds.), "Chapter 3.1 - Plant and Microbial Enzymes Involved in the Depolymerization of (1,3)-β-D-Glucans and Related Polysaccharides", Chemistry, Biochemistry, and Biology of 1-3 Beta Glucans and Related Polysaccharides, Academic Press, pp. 119–170, doi:10.1016/B978-0-12-373971-1.00004-2, ISBN 9780123739711
^ McCleary, Barry V. (1988-01-01). "Lichenase from Bacillus subtilis". Biomass Part A: Cellulose and Hemicellulose. Methods in Enzymology. Vol. 160. Academic Press. pp. 572–575. doi:10.1016/0076-6879(88)60170-4. ISBN 9780121820619.
^ Grishutin, Sergei G.; Gusakov, Alexander V.; Dzedzyulya, Ekaterina I.; Sinitsyn, Arkady P. (2006). "A lichenase-like family 12 endo-(1→4)-β-glucanase from Aspergillus japonicus: study of the substrate specificity and mode of action on β-glucans in comparison with other glycoside hydrolases". Carbohydrate Research. 341 (2): 218–229. doi:10.1016/j.carres.2005.11.011. PMID 16343463.
^ Hahn, Michael; Pons, Jaume; Planas, Antoni; Querol, Enrique; Heinemann, Udo (1995-10-30). "Crystal structure of Bacillus licheniformis 1,3-1,4-β- d -glucan 4-glucanohydrolase at 1.8 Å resolution". FEBS Letters. 374 (2): 221–224. Bibcode:1995FEBSL.374..221H. doi:10.1016/0014-5793(95)01111-Q. PMID 7589539.
^ Tsai, L.-C.; Shyur, L.-F.; Lee, S.-H.; Lin, S.-S.; Yuan, H.S. (2003-07-15). "Crystal structure of a natural circularly-permutated jellyroll protein: 1,3-1,4-β-D-glucanase from Fibrobacter succinogenes". Journal of Molecular Biology. 330 (3): 607–620. doi:10.1016/S0022-2836(03)00630-2. PMID 12842475.
^ ^a ^b Furtado, Gilvan Pessoa; Ribeiro, Lucas Ferreira; Santos, Camila Ramos; Tonoli, Celisa Caldana; de Souza, Angelica Rodrigues; Oliveira, Renata Rocha; Murakami, Mario Tyago; Ward, Richard John (2011-05-01). "Biochemical and structural characterization of a β-1,3–1,4-glucanase from Bacillus subtilis 168". Process Biochemistry. 46 (5): 1202–1206. doi:10.1016/j.procbio.2011.01.037. ISSN 1359-5113.

External links

Licheninase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

[1] Barras DR, Moore AE, Stone BA (1969). "Enzyme-Substrate Relationships Among β-Glucan Hydrolases". Cellulases and Their Applications. Advances in Chemistry. Vol. 95. pp. 105–138. doi:10.1021/ba-1969-0095.ch008. ISBN 0-8412-0095-5.

[2] "Lichenase endo-1-3-1-4-beta-D-Glucanase Bacillus subtilis". megazyme.com. Retrieved 2019-06-25.

[3] McCleary, Barry V; Codd, Rachel (1991). "Measurement of (1 → 3),(1 → 4)-β-D-glucan in barley and oats: A streamlined enzymic procedure". Journal of the Science of Food and Agriculture. 55 (2): 303–312. Bibcode:1991JSFA...55..303M. doi:10.1002/jsfa.2740550215. ISSN 0022-5142.

[4] Mangan, D.; Liadova, A.; Ivory, R.; McCleary, B. V. (2016-11-29). "Novel approaches to the automated assay of β-glucanase and lichenase activity". Carbohydrate Research. 435: 162–172. doi:10.1016/j.carres.2016.10.006. ISSN 0008-6215. PMID 27810709.

[5] Yoo, Dong-Hyung; Lee, Byung-Hoo; Chang, Pahn-Shick; Lee, Hyeon Gyu; Yoo, Sang-Ho (2007-03-01). "Improved Quantitative Analysis of Oligosaccharides from Lichenase-Hydrolyzed Water-Soluble Barley β-Glucans by High-Performance Anion-Exchange Chromatography". Journal of Agricultural and Food Chemistry. 55 (5): 1656–1662. Bibcode:2007JAFC...55.1656Y. doi:10.1021/jf062603l. ISSN 0021-8561. PMID 17284049.

[6] Hrmova, Maria; Fincher, Geoffrey B. (2009-01-01), Bacic, Antony; Fincher, Geoffrey B.; Stone, Bruce A. (eds.), "Chapter 3.1 - Plant and Microbial Enzymes Involved in the Depolymerization of (1,3)-β-D-Glucans and Related Polysaccharides", Chemistry, Biochemistry, and Biology of 1-3 Beta Glucans and Related Polysaccharides, Academic Press, pp. 119–170, doi:10.1016/B978-0-12-373971-1.00004-2, ISBN 9780123739711

[7] McCleary, Barry V. (1988-01-01). "Lichenase from Bacillus subtilis". Biomass Part A: Cellulose and Hemicellulose. Methods in Enzymology. Vol. 160. Academic Press. pp. 572–575. doi:10.1016/0076-6879(88)60170-4. ISBN 9780121820619.

[8] Grishutin, Sergei G.; Gusakov, Alexander V.; Dzedzyulya, Ekaterina I.; Sinitsyn, Arkady P. (2006). "A lichenase-like family 12 endo-(1→4)-β-glucanase from Aspergillus japonicus: study of the substrate specificity and mode of action on β-glucans in comparison with other glycoside hydrolases". Carbohydrate Research. 341 (2): 218–229. doi:10.1016/j.carres.2005.11.011. PMID 16343463.

[9] Hahn, Michael; Pons, Jaume; Planas, Antoni; Querol, Enrique; Heinemann, Udo (1995-10-30). "Crystal structure of Bacillus licheniformis 1,3-1,4-β- d -glucan 4-glucanohydrolase at 1.8 Å resolution". FEBS Letters. 374 (2): 221–224. Bibcode:1995FEBSL.374..221H. doi:10.1016/0014-5793(95)01111-Q. PMID 7589539.

[10] Tsai, L.-C.; Shyur, L.-F.; Lee, S.-H.; Lin, S.-S.; Yuan, H.S. (2003-07-15). "Crystal structure of a natural circularly-permutated jellyroll protein: 1,3-1,4-β-D-glucanase from Fibrobacter succinogenes". Journal of Molecular Biology. 330 (3): 607–620. doi:10.1016/S0022-2836(03)00630-2. PMID 12842475.

[:0-11] Furtado, Gilvan Pessoa; Ribeiro, Lucas Ferreira; Santos, Camila Ramos; Tonoli, Celisa Caldana; de Souza, Angelica Rodrigues; Oliveira, Renata Rocha; Murakami, Mario Tyago; Ward, Richard John (2011-05-01). "Biochemical and structural characterization of a β-1,3–1,4-glucanase from Bacillus subtilis 168". Process Biochemistry. 46 (5): 1202–1206. doi:10.1016/j.procbio.2011.01.037. ISSN 1359-5113.

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